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Pawpaw extract with anti-cancer activity, and preparation method and application thereof

A technology of papaya extract and anticancer activity, which is applied in the direction of medical preparations containing active ingredients, diseases, drug combinations, etc., can solve the problems of not disclosing the specific use of papaya seed oil, etc., and achieve simple preparation method and good activity Effect

Inactive Publication Date: 2013-04-17
CHONGQING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent (200910192336.1) discloses a process for extracting papaya seed oil using SCFES (supercritical process), but the patent does not disclose the specific use of papaya seed oil

Method used

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  • Pawpaw extract with anti-cancer activity, and preparation method and application thereof
  • Pawpaw extract with anti-cancer activity, and preparation method and application thereof
  • Pawpaw extract with anti-cancer activity, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0016] The preparation method investigation of embodiment 1 papaya extract

[0017] Wash the papaya, slice it, remove the seeds, dry it naturally, crush it, sieve it, and take the 60-mesh powder for later use. Weigh an appropriate amount of papaya powder and put it into the barrel, and the amount of entraining agent is 2mL g -1 Add the corresponding amount of carrier agent to the carrier agent tank, place the barrel in the extraction kettle, adjust the temperature and pressure of the extraction kettle and separation kettle to corresponding values, press the carrier agent into the extraction cylinder, and adjust the CO 2 , the flow rate is 15L·h -1 After 80 minutes of extraction, open the valve of the separation kettle to release the extract, add water to the extract at a ratio of 1:1 between the extract and water, and recover the extract at 50±5°C until it has no alcohol smell and is lower than 10°C After cooling and standing for more than 1 hour, the filter residue was coll...

Embodiment 2

[0027] Example 2 Study on the Activity of Papaya Extract on Gastric Cancer

[0028] Gastric cancer cell line (BGC823) was cultured in RPMI1640 medium (containing 10%-20% fetal bovine serum, 100 U / mL of penicillin and streptomycin, and 0.03% glutamine) at 37°C, saturated humidity, 5CO 2 In the incubator, change the medium every other day. After the cells enter the logarithmic growth phase, discard the supernatant of the adherent cells, wash them twice with phosphate buffer, and digest the cells with 0.25% trypsin (the cells that grow in suspension are collected directly) , and then rinsed twice with phosphate buffered saline, and adjusted the cell suspension concentration to 1×10 cells / mL with RPMI1640 complete medium for use in experiments.

[0029] The influence of papaya extract aqueous solution (RAE) described in embodiment 1 on human gastric cancer cell BGC823 cell proliferation: the tumor cell strain suspension is inoculated in 96-well culture plate, every hole 100td (con...

Embodiment 3

[0039] Example 3 Study on the Activity of Papaya Extract on Breast Cancer

[0040] MDA-MB-231 cells were inoculated in RPMI1640 medium containing 10% fetal bovine serum at 37°C, 5% CO 2 Under normal conditions, the medium was changed every 2-3 days, and the cells were subcultured when the bottom of the bottle was about 85% full, and the cells in the logarithmic growth phase were taken for experiments. Take the cells in the logarithmic growth phase and make 1×10 5 ·ml -1 The cell suspension was inoculated in a 96-well plate, 90 μl per well, cultured for 24 hours, and then added papaya extract with final concentrations of 75, 150, 300, and 600 μg·ml, and the control group was added with an equal volume of RPMI1640 culture solution Treatment (4 replicate wells for each concentration), after 24, 48, and 72 hours of treatment for each concentration, 10 μl of CCK8 was added to culture for 1 hour, and the absorbance A value of each well was detected by a microplate reader at a wave...

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Abstract

The invention relates to a preparation method of pawpaw extract with anti-cancer activity. The preparation method comprises the following steps of: extracting dried pawpaw by adopting an SCFES method to obtain crude extract, and purifying the crude extract by adopting a water precipitation process to obtain the pawpaw extract. The preparation method of pawpaw extract, provided by the invention, is simple and applicable to large-scale industrial production, and the purified pawpaw extract has good activity when used for treating gastric cancer and breast cancer.

Description

technical field [0001] The invention relates to an extract of traditional Chinese medicinal materials, in particular to an extract of papaya with anticancer activity, a preparation method and application thereof. technical background [0002] Papaya is the dry and mature fruit of Rosaceae plant Chaenomeles speciosa S.Nakai. It is mainly produced in Anhui, Shandong, Zhejiang, Hubei, Yunnan, Guizhou, Sichuan and other places. ", "Saffron" and known as my country's four famous Chinese herbal medicines. As a commonly used traditional Chinese medicine, papaya has a sour and warm taste, and has the effects of calming the liver and relaxing tendons, harmonizing stomach and eliminating dampness. As the saying goes, "one is beneficial for apricot, two for pear, and all for papaya". It is believed that papaya has the effects of relaxing tendons, activating collaterals, invigorating the spleen and appetizing, soothing the liver and relieving pain, and expelling wind and dampness. It ca...

Claims

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Application Information

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IPC IPC(8): A61K36/732A61P35/00A61P15/14A61P1/00
Inventor 杨宪张雪杨水平余晓东和七一兰作平唐倩
Owner CHONGQING NORMAL UNIVERSITY
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