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Vaccine composition as well as preparation method and application thereof

A technology of vaccine composition and antigen, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, virus antigen components, etc., and can solve problems such as simultaneous immune interference

Active Publication Date: 2013-04-17
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In order to solve the deficiencies in the prior art, the purpose of the present invention is to provide a vaccine composition for treating and preventing swine fever and porcine blue-ear disease, to solve the problem that existing vaccines interfere with simultaneous immunization, and to provide the vaccine composition The optimal ratio of the two types of antigens in

Method used

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  • Vaccine composition as well as preparation method and application thereof
  • Vaccine composition as well as preparation method and application thereof
  • Vaccine composition as well as preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0031] The preparation of embodiment 1 swine fever virus antigen liquid

[0032] 1. The highly sensitive ST cells (purchased from ATCC) containing 0.125% trypsin and 0.03 %EDTA digestion solution, digest and disperse, inoculate cell culture flasks at a suitable density after counting cells, add 1.5-5% FBS MEM cell culture solution, and add seed poison at the same time according to M.O.I. The inoculation dose is M.O.I.=0.1-0.6, more preferably the inoculation dose is M.O.I.=0.2-0.4, and cultured in an incubator at 34-37°C, more preferably at a temperature of 34-35°C.

[0033] 2. After three days of cultivation, the first time of poisoning is carried out. After the poisoning, the cell maintenance solution of 1.5-3% FBS is added. After that, the poison is collected every 2 days, and the poison can be collected 5 times continuously. After harvesting the virus, the antigens were mixed and stored at -20°C.

[0034] 3. Mix and sample the virus fluid harvested five times before perf...

Embodiment 2

[0035] The preparation method of embodiment 2 highly pathogenic mutant strain antigen of PRRS

[0036] 1. Passage and culture of cells for seedling production: Disperse and passage Marc145 or MA104 cell lines with EDTA-trypsin digestion solution, and continue to culture with cell growth medium.

[0037] 2. The propagation of cytotoxic species: the virus liquid of PRRS HuN4-F112 strain (commercially available) is inserted into the cell bottle that has grown into a good cell monolayer by the dose of M.O.I.=0.001-0.01, and the dose of poisoning is further optimized M.O.I. = 0.005-0.01. After 1 hour of adsorption, add cell maintenance solution to the cell line monolayer and continue to culture. Harvest when 70-80% of the cells have lesions. Save as below, take a small amount for inspection of semi-finished products, the virus content of the antigen solution is 10 6.5 TCID 50 / ml, in accordance with the national standard inspection are in compliance with the regulations.

[0038...

Embodiment 3

[0040] Example 3 Double vaccine composition prepared after different ratios of swine fever, porcine highly pathogenic PRRS (HuN4-F112, JXA1-R, TJM-F92) antigens and swine fever virus antigens

[0041] The hog fever virus antigen prepared by embodiment 1 (virus content 10 6.5 TCID 50 / ml), three kinds of porcine PRRS virus antigens prepared in embodiment 2 carry out proportioning according to the antigen content of table 1 respectively, then mix with the freeze-drying protection agent of equal amount, make porcine PRRS, swine fever double See Table 1 for the live vaccine vaccine composition.

[0042] Table 1 Different ratios and groups of PRRS antigens and CSF antigens

[0043]

[0044] Mix the above antigens in sequence, then add a mixture of 2wt% gelatin and 15wt% lactose in a volume ratio of 1:1 as a freeze-drying protective agent, stir and mix for 1 hour, carry out aseptic subpackaging, and store at 2-8°C ,spare.

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Abstract

The invention provides a vaccine composition. The vaccine composition comprises hog cholera virus antigen and porcine reproductive and respiratory syndrome (PRRS) antigen, wherein the hog cholera virus antigen is a hog cholera rabbit attenuated strain, the PRRS antigen is a high-pathogenicity PRRS virus, and the content ratio of the PRRS antigen and the hog cholera virus antigen is 100:1-100:10. The vaccine composition can eliminate the interference between the two types of antigen, achieves the purpose of preventing two infectious diseases by one injection, and greatly reduces immunization times and animal stress response.

Description

technical field [0001] The invention relates to a vaccine composition for preventing and treating swine fever and pig blue-ear disease. Background technique [0002] Classical swine fever (CSF) is a devastating infectious disease caused by classical swine fever virus (CSFV), which seriously harms the swine industry. The mortality rate of this disease is as high as 80-90%. The World Organization for Animal Health (OIE) lists it as an OIE disease list, and my country also lists it as a class of infectious disease. The prevention and control of swine fever has always been a topic that countries with swine fever in the world attach great importance to. For this reason, countries have invested a lot of manpower and material resources. Vaccination is still an important means of controlling swine fever. The attenuated swine fever vaccines produced in my country include splenocytes, milk rabbit tissue vaccines, bovine testicular primary cell vaccines and pig testicular passage cel...

Claims

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Application Information

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IPC IPC(8): A61K39/187A61K39/12A61P31/14
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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