Method for determining best ice temperature cold induction mode of fish flesh
A cold induction, fish meat technology, applied in the direction of preservation of meat/fish by freezing/cooling, investigation stage/state change, etc., to achieve the effect of maintaining freshness, prolonging storage time, and increasing taste
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Embodiment 1
[0050] 1 Materials and methods
[0051] 1.1 Materials
[0052] Tilapia: Purchased from the Guzong Road Vegetable Market in Lingang New City, Shanghai, weighing 500-600g / tail, oxygenated and quickly transported back to the laboratory after purchase.
[0053] 1.2 Reagents and equipment
[0054] Reagents: Standards include Adenosine Triphosphate (ATP) and its compounds Adenosine Diphosphate (ADP), Adenosine Monophosphate (AMP), Inosine Monophosphate (IMP), Inosine ( Inosine, HxR), hypoxanthine (Hypoxanthine, Hx), ATP, ADP, IMP, Hx standard products were produced by Sigma Company, AMP standard products were produced by Japan TCI Company, HxR standard products were produced by German Dr. Ehrenstorfer Company. Methanol is HPLC grade (Sinopharm Group Chemical Reagent Co., Ltd.); Phosphate HPLC grade (Shanghai Anpu Scientific Instrument Company); experimental water is ultrapure water; perchloric acid (PCA), potassium hydroxide, sodium hydroxide, phosphoric acid , sulfosalicylic aci...
Embodiment 2
[0113] 1 Materials and methods
[0114] 1.1 Materials and equipment
[0115] Tilapia: In August 2012, tilapia was purchased from the Guzong Road Vegetable Market in Lingang New Town, Shanghai, with a weight of 500-600g / tail. After purchase, it was oxygenated and quickly transported back to the laboratory. Store the live fish in a preservation box for 1~2 hours to prevent them from struggling, then beat the live fish to death, slice them into fish fillets, put them in fresh-keeping bags and place them in a cold environment; adenosine triphosphate (ATP), diphosphate Adenosine (ADP), inosinic acid (IMP), hypoxanthine (Hx): Sigma Company; adenosine monophosphate (AMP): Japan TCI Company; inosine (HxR): German Dr.Ehrenstorfer Company; dihydrogen phosphate Potassium, potassium dihydrogen phosphate: Shanghai Anpu Scientific Instrument Co., Ltd., chromatographically pure; ultrapure water, perchloric acid (PCA), potassium hydroxide, sodium hydroxide, phosphoric acid, sulfosalicylic ac...
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