Method for preparing peanut peptide by microbial solid fermentation of peanut meal

A technology of solid-state fermentation and peanut meal, applied in the protein composition of vegetable seeds, food science, protein food components, etc., to achieve the effect of low investment scale and production cost, significant economic and social benefits

Inactive Publication Date: 2013-04-24
杨庆利
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems existing in the existing liquid fermentation technology, the present i

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 The method for preparing peanut peptides by microbial solid-state fermentation of peanut meal in this example, the specific steps are as follows:

[0014] 1. Selection and activation of microbial strains

[0015] Select Bacillus subtilis, Candida tropicalis, Aspergillus niger and Streptococcus lactis with strong enzyme production ability and effective complementation, and activate the culture:

[0016] Inoculate Bacillus subtilis strains in LB culture at 30°C, shake culture for 48 hours, and set aside;

[0017] Candida tropicalis strains were inoculated in LB medium for shaking flask culture, culture temperature was 35°C, shake culture for 48 hours for later use;

[0018] Aspergillus niger was inoculated in PDA culture based on static culture at 37°C for 36 hours, and set aside;

[0019] Streptococcus lactis was inoculated in MRS culture based on static anaerobic culture at 37°C for 72 hours, and set aside;

[0020] 2. Preparation of mixed fermentation stra...

Embodiment 2

[0025] Example 2 The method for preparing peanut peptides by microbial solid-state fermentation of peanut meal in this example, the specific steps are as follows:

[0026] 1. Selection and activation of microbial strains

[0027] Select Bacillus subtilis, Candida tropicalis, Aspergillus niger and Streptococcus lactis with strong enzyme production ability and effective complementation, and activate the culture:

[0028] Inoculate Bacillus subtilis strains in LB culture at 30°C, shake culture for 48 hours, and set aside;

[0029] Candida tropicalis strains were inoculated in LB medium shake flask culture, the culture temperature was 35°C, shake culture for 48h, and set aside;

[0030] Aspergillus niger was inoculated in PDA culture based on static culture at 37°C for 36 hours, and set aside;

[0031] Streptococcus lactis was inoculated in MRS culture based on static anaerobic culture at 37°C for 72 hours, and set aside;

[0032] 2. Preparation of mixed fermentation strains

...

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PUM

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Abstract

The invention belongs to the technical field of peanut protein processing, and specifically relates to a method for preparing peanut peptide by microbial solid fermentation of peanut meal. The method comprises the following steps of: choosing and activating a bacterial strain; preparing mixed fermentation bacteria; preparing a solid fermentation culture medium; and performing solid fermentation, crude product acquisition, separation, vacuum concentration and freeze drying to obtain the finished peanut peptide. By adopting the method for preparing the peanut peptide by the microbial solid fermentation of the peanut meal, difficulties such as large amounts of wastewater and serious pollution after the extraction of fermented liquid product related to a traditional liquid fermentation method are solved; the method has the unique advantages of water conservation and energy conservation without producing the wastewater, and belongs to a clean production technology; the investment scale and the production cost of the method provided by the invention are lower than those of the liquid method; and obvious economic benefit and social benefit are brought by the method.

Description

technical field [0001] The invention belongs to the technical field of peanut protein processing, and in particular relates to a method for preparing peanut peptides by microbial solid-state fermentation of peanut meal. Background technique [0002] Peanut is an important oil crop and edible crop in my country, and its output and export volume rank first in the world. At present, China's utilization of peanuts is mostly in the extraction of edible oils, and the utilization of peanut proteins is less. Peanut protein is the remaining substance after extracting oil and fat from peanuts, also known as peanut meal. The protein content of peanuts is 25% to 30%. Peanut protein contains eight kinds of amino acids necessary for the human body. The content of arginine is higher than that of other nuts, and its biological value is higher than that of soybeans. At present, the peanut protein products on the domestic market are mainly peanut protein powder, which is generally used as t...

Claims

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Application Information

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IPC IPC(8): A23J1/14
Inventor 杨庆利孙杰于丽娜朱凤张初署毕洁魏明秀明强强
Owner 杨庆利
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