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Cell micro-array chip and preparation method thereof

A microarray chip, cell technology, applied in biochemical equipment and methods, microbial libraries, enzymology/microbiology devices, etc., to achieve the effect of easy operation

Inactive Publication Date: 2013-04-24
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, most of the existing technologies use silicon, glass, or plastic as base materials, and assemble cells into On the surface of the base material, the cell microarray chip is prepared, the production process is complicated, and it is not convenient for continuous operation at the same time

Method used

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  • Cell micro-array chip and preparation method thereof

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Effect test

Embodiment 1

[0021] Embodiment 1: Prepare the microarray chip of HepG2 cell on alginic acid gel

[0022] HepG2 cells are a type of liver cancer cell line.

[0023] The first step is to prepare the alginic acid hydrogel template: apply the prepared sodium alginate solution with a mass concentration of 1.0 g / L on the glass plate, and then quickly immerse the glass plate in chlorine with a mass concentration of 10 g / L In the calcium chloride solution, take it out after soaking for 30 minutes, and a layer of alginic acid gel will be formed on the glass plate, which is called the alginic acid hydrogel template.

[0024] The second step is to prepare a polydimethylsiloxane lattice template: the lattice template has a size of 30×30 mm, and a plurality of cylinders are arranged on it, and the interface diameter of the cylinders is 200 μm. The distance between them was 600 μm, and then the top of the template was soaked in a chitosan solution with a mass concentration of 0.5 g / L for 30 minutes.

...

Embodiment 2

[0027] Example 2: Preparation of a microarray chip of CHO cells on an alginate gel

[0028] CHO cells are a cell line of Chinese hamster ovary cells.

[0029] The first step is to prepare the alginic acid hydrogel template: apply the prepared sodium alginate solution with a mass concentration of 2.0 g / L on the glass plate, and then quickly immerse the glass plate in chlorine with a mass concentration of 15 g / L In the calcium chloride solution, take it out after soaking for 20 minutes, and a layer of alginic acid gel will be formed on the glass plate, which is called the alginic acid hydrogel template.

[0030] The second step is to prepare a polydimethylsiloxane lattice template: the lattice template has a size of 30×30 mm, and a plurality of cylinders are arranged on it, and the interface diameter of the cylinders is 200 μm. The distance between them was 600 μm, and then the top of the template was soaked in a chitosan solution with a mass concentration of 3.0 g / L for 5 minu...

Embodiment 3

[0033] Embodiment 3: prepare the microarray chip of McF7 cell on alginic acid gel

[0034] McF7 cells are a type of breast cancer cell line.

[0035] The first step is to prepare the alginic acid hydrogel template: apply the prepared sodium alginate solution with a mass concentration of 4.0 g / L on the glass plate, and then quickly immerse the glass plate in chlorine with a mass concentration of 30 g / L In the calcium chloride solution, take it out after soaking for 5 minutes, and a layer of alginic acid gel will be formed on the glass plate, which is called the alginic acid hydrogel template.

[0036] The second step is to prepare a polydimethylsiloxane lattice template: the lattice template has a size of 30×30 mm, and a plurality of cylinders are arranged on it, and the interface diameter of the cylinders is 200 μm. The distance between them was 600 μm, and then the top of the template was soaked in a polylysine solution with a mass concentration of 0.05 g / L for 30 minutes. ...

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Abstract

The invention provides a cell micro-array chip and a preparation method thereof and belongs to the technical field of cell chips. The cell micro-array chip is characterized by being prepared by using alginate gels; and the preparation method comprises the following steps: 1, coating a layer of sodium alginate solution on a glass plate, rapidly immerging the glass plate in a calcium chloride solution and soaking the glass plate for 5-30 minutes to form a gel template; 2, preparing a dot matrix template on which a plurality of cylinders are arranged, soaking the top ends of the cylinders in a chitosan or polylysine solution and taking out the cylinders after 5-30 minutes; 3, embossing the top ends of the cylinders on the alginate gel template and forming a complex dot matrix template through electrostatic complexation reaction; and 4, soaking the complex dot matrix template in a culture solution with cell concentration of 1*10<5>-1*10<7> and forming the alginate gel cell micro-array chip when the complex dot matrix template is cultured for 4-16 hours. The cell micro-array chip provided by the invention has the advantage of simplicity and convenience in operation and can be used for cell tissue like culturing, high throughput screening of compounds or medicines, discovery of new genes, proteomic studies and the like.

Description

Technical field: [0001] The invention belongs to the technical field of cell chips, and in particular relates to a cell microarray chip and a preparation method thereof. Background technique: [0002] Biochips mainly assemble biologically active substances on solid substrates to form microarrays to achieve accurate, rapid and large-scale information screening or detection of compounds (such as drugs), proteins, cells and other biological components. Cell microarray chip is a kind of biochip, that is, through micro-processing technology, a detection plate with higher density and smaller aperture is manufactured on a substrate such as silicon, glass, or plastic, and cells are fixed in the hole. The maturity of this technology is of great significance for the development of new drugs, the detection of food, environment and life sciences. [0003] At present, the existing technologies mostly use silicon, glass, or plastic as the base material, and cells are assembled on the su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C40B40/02C40B50/14
Inventor 黄晓波王婵杭瑞强唐宾
Owner TAIYUAN UNIV OF TECH
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