Culture medium for promoting vibrio parahemolyticus to enter non-culturable state and method
A technology of Vibrio hemolyticus and culture medium, applied in the field of microbial isolation and culture, can solve the problems of increasing scientific research costs, delaying scientific research progress, etc., and achieves the effects of saving scientific research costs, short induction time, and simple preparation
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[0022] Example 1
[0023] (1) Preparation of culture medium that promotes the entry of Vibrio parahaemolyticus into a live, non-cultivable state
[0024] Weigh 10g of peptone, 3g of beef extract, 30g of sodium chloride, 0.1g of potassium sorbate, add distilled water to 1000mL, adjust the pH to 7.4 with 1M HCl and 1M NaOH, sterilize at 121℃, 1.05MPa for 15min to obtain the promoted parahaemolytic arc The bacteria enter the liquid medium in a live, non-cultivable state.
[0025] Weigh 10g peptone, 3g beef extract, 30g sodium chloride, 0.1g potassium sorbate, 15g agar, add distilled water to 1000mL, adjust pH to 7.4 with 1M HCl and 1M NaOH, sterilize at 121℃, 1.05MPa for 15min to promote parahemolysis Vibrio enters a solid medium in a live, non-culturable state.
[0026] (2) Rapid induction of viable non-culturable Vibrio parahaemolyticus
[0027] 1) The standard strain of Vibrio parahaemolyticus GMCC1.1614 (purchased from China Collection of Common Microorganisms) was inoculated into co...
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[0033] Example 2
[0034] (1) Preparation of culture medium that promotes the entry of Vibrio parahaemolyticus into a live, non-cultivable state
[0035] Weigh 10g of peptone, 3g of beef extract, 30g of sodium chloride, 1g of potassium sorbate, add distilled water to 1000mL, adjust the pH to 7.4 with 1M HCl and 1M NaOH, sterilize at 121℃, 1.05MPa for 15min to obtain promoted Vibrio parahaemolyticus A liquid medium that enters a live, non-cultivable state.
[0036] Weigh 10g of peptone, 3g of beef extract, 30g of sodium chloride, 1g of potassium sorbate, 20g of agar, add distilled water to 1000mL, adjust the pH to 7.4 with 1M HCl and 1M NaOH, and sterilize at 121℃, 1.05MPa for 15min to obtain the accelerated parahaemolytic arc The bacteria enter the solid medium in a live, non-cultivable state.
[0037] (2) Rapid induction of viable non-culturable Vibrio parahaemolyticus
[0038] According to the method of Example 1 (2), the liquid medium prepared in Example 2 was used for induction at...
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