Culturing method capable of promoting grease accumulation in chlorella

A technique for oil accumulation and cultivation method, which is applied in the field of cultivation that can promote oil accumulation in Chlorella, and achieves the effects of increasing oil content, improving growth and oil accumulation, and being inexpensive.

Active Publication Date: 2014-08-27
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Salicylic acid and brassinolide are two types of plant hormones that exist in trace amounts in plants and have a significant effect on enhancing plant stress resistance. They are closely related to the stress resistance of algae and the accumulation of secondary metabolites. These two plant hormones Both may increase the accumulation of metabolites of chlorella, but there is no relevant research report on the use of salicylic acid or brassinosteroids to improve the growth and oil accumulation of chlorella

Method used

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  • Culturing method capable of promoting grease accumulation in chlorella
  • Culturing method capable of promoting grease accumulation in chlorella
  • Culturing method capable of promoting grease accumulation in chlorella

Examples

Experimental program
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Effect test

Embodiment 1

[0018] The invention discloses a method for promoting oil accumulation of chlorella. First, the chlorella pyrenoidosa F-9 (C.pyrenoidosa) was quickly cultured to the late logarithmic growth period under better growth conditions, and the chlorella reached a higher density, and the OD was determined 460 =1.47. Fully dissolve chemically pure salicylic acid (SIGMA company) with a certain amount of ethanol to make a mother solution with a concentration of 1.0 mg / mL, and add the mother solution to the medium in the late logarithmic growth period, so that the final concentrations are 0.1, 0.5 , 1.0 μg / mL; the chlorella added with salicylic acid was continuously cultured at 25°C, with a light intensity of 4000lx, a photoperiod of 12 hours of continuous light and 12 hours of continuous darkness per day, and without the addition of exogenous medium. Daily detection of chlorella oil content (the detection method of chlorella oil is vanillin colorimetric method, the method refers to Cong...

Embodiment 2

[0022] It is basically the same as Example 1, except that the phytohormone uses 24-epibrassinolide (Shanghai Yuanye Biotechnology Co., Ltd.). After testing, the optimal final concentrations are 2.5, 5.0, and 10.0 μg / mL respectively. The obtained oil content accumulation data of Chlorella are shown in Table 2, and all the data are the average value of three determinations. Results The oil content of Chlorella added with 24-epibrassinolide was the highest at 35.8%, which was 132.1% of the control group.

[0023] Table 2 Changes in oil content in Chlorella after the control and the addition of 24-epibrassinolide (%)

[0024]

Embodiment 3

[0026] It is basically the same as in Example 1, except that the phytohormone uses salicylic acid (0.25 μg / mL final concentration in the culture medium) and 24-epibrassinolide (1.25 μg / mL) at the same time, and the obtained small The accumulation data of the oil content of the algae are shown in Table 3, and all the data are the average value of three measurements. Results The oil content of Chlorella added with salicylic acid and 24-epibrassinolide was 37.5%, which was 138.3% of the control group.

[0027] Table 3 Changes of oil content in chlorella after the control and adding two kinds of phytohormones (%)

[0028]

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Abstract

The invention discloses a culturing method capable of promoting grease accumulation in chlorella. The culturing method is characterized by comprising the following steps of: (1) dissolving salicylic acid or 24-epibrassinolide in ethanol to prepare a first mother solution and a second mother solution having the corresponding concentrations of 0.5-2 mg / mL; (2) culturing the chlorella until an OD (Outside Diameter) value reaches to 0.1-3.0; (3) adding the first mother solution, the second mother solution or both the first mother solution and the second mother solution into a chlorella culture solution according to a proportion; and (4) continuously illuminating the culture solution in which the first mother solution, the second mother solution or the mixture of the first mother solution and the second mother solution is added under the conditions of 25 DEG C, 40001x of the light intensity and 12 hours every day of the photoperiod and then continuously culturing for 12 hours under in a dark environment; and culturing the chlorella until the color of the chlorella is changed from green to yellow so as to obtain the chlorella with a relatively high content of the grease. The culturing method capable of promoting the grease accumulation in the chlorella has the advantages of increasing the grease accumulation speed of the chlorella efficiently and increasing the grease content of the chlorella obviously.

Description

technical field [0001] The invention relates to a method for cultivating chlorella, in particular to a method for promoting oil accumulation in chlorella. Background technique [0002] Chlorella is a kind of energy microalgae with high oil content and great application prospects. It not only has a high protein content, but also has the characteristics of fast growth rate and high oil content. It also has a relatively mature large-scale cultivation process and technical system in my country. It is a kind of economical microalgae that has been studied more at present. The use of microalgae such as chlorella to rapidly grow and accumulate oil to produce biodiesel is a hot spot in the research and development of new energy sources. At present, there are many literatures and patents on chlorella cultivation systems, such as bioreactor cultivation, open track pond cultivation, cultivation conditions, processes, and culture media. However, as a bioenergy source, chlorella also ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12R1/89
Inventor 徐年军孙雪陆海波丛峰江灵芝
Owner NINGBO UNIV
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