Preparation method for chymotrypsin

Abstract

The invention provides a preparation method for chymotrypsin. The preparation method comprises the following process procedures: (1), grinding raw material and extracting protein; (2) salting out in a grading manner to obtain a crude product of chymotrypsin; (3), feeding the crude product and crystallizing in a multiple manner; (4), activating; (5), salting out; (6), dialyzing; and (7), freeze-drying. According to the invention, a set of large-scale manufacturing technology is established and has the advantages of simplicity, low cost and the like; the titer of a fine product of prepared chymotrypsin is more than 1,200 unit / milligram; and the prepared chymotrypsin meets the requirements of Chinese pharmacopeia (2010) and has very great market competitiveness.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and designs a biochemical separation technology of medicinal chymotrypsin. Specifically, techniques such as salting out, multiple crystallization combined with dialysis are used to extract chymotrypsin. Background technique [0002] Chymotrypsin is a protease isolated and purified from bovine pancreas or porcine pancreas. Its pharmacological and clinical functions mainly include two aspects: 1. Anti-inflammation: it has good curative effect on various inflammations, inflammatory edema, hematoma, ulcer and thrombus. 2. Anti-cancer: It can promote the penetration of anti-cancer drugs into the lesion, promote the effect of chemotherapy drugs, and the effect is stronger when used locally in large doses. Large-dose use can treat a variety of tumors, such as breast cancer, cervical cancer, gastric cancer, etc., without any toxic side effects or adverse reactions. [0003] Chinese patent CN1944641A ...

AI Technical Summary

Problems solved by technology

[0003] Chinese patent CN1944641A discloses a preparation method of chymotrypsin, which uses an affinity chromatography process to produce high-purity chymotrypsin from prochymotrypsin, but as analyzed in Chinese patent CN1013025B, there are also some shortcomings. The most important one is the affinity chromatography medium Expensive, less reusable times, large amount of medium used, not suitable for large-scale production

Although the Chinese patent CN1013025B has solved the deficiency of affinity chromatography, it can also obtain high specific activity chymotrypsin, but similarly, compared with the traditional process, the production cost is too high