Antimicrobial formulation

An antimicrobial, compositional technology, applied in the direction of biocides, chemicals for biological control, animal repellants, etc., that can solve problems such as prevention of mold growth that does not indicate

Inactive Publication Date: 2013-04-24
ANITOX CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These four patents refer to the addition of ethoxylated castor oil surfactants, preferably emulsions, to improve the digestibility of hydrop

Method used

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Examples

Experimental program
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Effect test

Embodiment 1 and 2

[0088] Procedure: The following formulations were prepared for repeat in vitro studies. All reagents are of the highest purity and laboratory grade. For acetic acid, a 56% solution was prepared. Due to solubility issues, dilute succinic acid to a 5% solution in water. For comparison purposes, two commercial products, a formic acid / propionic acid blend and a formaldehyde / propionic acid blend, were tested.

[0089]

[0090] *CO-60 is an ethoxylated castor oil surfactant with 60 ethylene units.

[0091] A suspension of S. typhimurium was added to two tubes containing 0.05% (500 ppm) of each formulation. The tubes were vortexed and incubated for 24 hours at room temperature, then the solution was plated on SMA (Standard Method Agar) for 48 hours before counting Salmonella colonies.

[0092] Results: The following table shows that several formulations were effective in controlling the growth of Salmonella.

[0093] Results: Study 1 and Study 2

[0094]

[0095] Conclusi...

Embodiment 3

[0097] Procedure: From previous in vitro studies, six formulations were selected to test their effectiveness against Salmonella in feed. For comparison purposes, a formaldehyde / propionic acid blend was tested. Poultry meal feed was modified with a meat and bone meal inoculum of S. typhimurium. The contaminated feed was then treated with one of the following formulations at 1 kg / MT, 2 kg / MT and 3 kg / MT. After 24 hours, a subsample of 10 g of the treated feed was suspended in 90 ml of Butterfield's buffer. Dilutions were plated on XLT-4 agar and incubated at 37°C for 48 hours before counting Salmonella colonies. The formulations used in this experiment are shown in the table below.

[0098]

[0099] *CO-60 is an ethoxylated castor oil surfactant with 60 ethylene units.

[0100] Results: The table below shows that several formulations were effective in controlling the growth of Salmonella.

[0101] deal with

[0102] Conclusions: Formulations containing trans-2...

Embodiment 4

[0104] Procedure: Five formulations were selected to test their effectiveness against S. typhimurium in feed. Modification of poultry meal feed with a meat and bone meal inoculum of Salmonella typhimurium. The contaminated feed was then treated with one of the following formulations at 1 kg / MT, 2 kg / MT and 3 kg / MT. After 24 hours, a subsample of 10 g of the treated feed was suspended in 90 ml of Butterfield's buffer. Dilutions were plated on XLT-4 agar and incubated at 37°C for 48 hours before counting Salmonella colonies. Additional samples were taken 7 and 14 days after Salmonella enumeration treatment. The formulations used are shown in the table below.

[0105]

[0106] *CO-60 is an ethoxylated castor oil surfactant with 60 ethylene units.

[0107] Results: The table below shows that several formulations were effective in controlling Salmonella.

[0108]

[0109] Conclusions: All formulations resulted in a reduction in the number of Salmonella in the feed. Form...

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Abstract

An anti-microbial composition for treating animal feed, comprising: 1 to 90 wt.%, based on the total weight, of a Ci to C24 organic acid, 1 to 90 wt.%), based on the total weight, of trans-2-hexenal provided it constitutes at least 5 wt.% of the organic acid and aldehyde mixture, 0 to 50 wt.%) based on the total weight of terpenes, 0 to 20 wt.%) based on the total weight of a surfactant, and water.

Description

technical field [0001] A synergistic antimicrobial formulation comprising a mixture of organic acids, α,β-unsaturated fatty aldehydes and antimicrobial terpenes. Background technique [0002] Food borne illness costs the U.S. $152 billion a year in health-related expenses, according to recent research by the Pew Charitable Trusts of Georgetown University. According to the U.S. Centers of Diseases Control and Prevention, an estimated 76 million people become ill and 5000 die each year in the United States from foodborne illness (Thomson Reuters 2010, 3 / 10 / 2010). [0003] The desire to find more natural and / or organic antimicrobials has generated a great deal of research and increased raw material costs due to the low commercial availability of these new natural / organic products. [0004] Several new antimicrobial agents have been discovered in plants. We have found that when grass or other plants are damaged by cutting, pruning or exposure to pathogens, the lipoxygenase pat...

Claims

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Application Information

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IPC IPC(8): A23K1/17A23K1/16A01N61/00A01P1/00A23K20/195
CPCA01N31/16A23K1/1826A23K1/1646A01N37/02A23K1/1612A01N35/02A01N31/08A01N37/42A23K50/75A23K20/10A23K20/111A01N2300/00A23K20/195A01N61/00
Inventor 胡里奥·皮门特尔
Owner ANITOX CORP
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