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A kind of anti-mosquito gel and preparation method thereof

A mosquito gel and gel technology are applied in the field of mosquito-killing gel, which can solve the problems of poor vitality, difficult to meet practical application and the like, and achieve the effects of long duration, good application prospect and good mosquito-killing activity.

Inactive Publication Date: 2015-08-05
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Pythium can effectively kill the highly dense mosquito larvae, larvae, which are still in the aquatic stage, and can effectively cut off the way for mosquitoes to transmit diseases. However, its application in killing mosquitoes is still in its infancy. Mosquito killing can only be done under the condition of vigor, directly making Pythium into mosquito killing products, the vigor is poor, it is difficult to meet the needs of practical application, at present, there is no report to use the mosquito killing products prepared by this Pythium

Method used

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  • A kind of anti-mosquito gel and preparation method thereof
  • A kind of anti-mosquito gel and preparation method thereof
  • A kind of anti-mosquito gel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 mosquito-killing gel of the present invention

[0032] Take 1 / 4KPYG 2 Medium (composition: peptone 0.375g, yeast extract 0.125g, glucose 0.75g, cholesterol 6.25mg, calcium chloride 27.5mg, corn oil 2.5g, add water to 1L), add trehalose 10g, corn oil 25g, OP-100.5 g, adjust the pH value to 7.0~10.0, add 1g of agar powder, dissolve, cool to 60°C, add Pythium sp.GY1938 spores until the number is 5×10 10 Homogenize and stir to obtain a gel suspension, put it in a mold immediately, cut into pieces to shape after solidification, and package and store at 4-8°C.

Embodiment 2

[0033] Embodiment 2 The preparation of mosquito-killing gel of the present invention

[0034] Take 2×KPYG 2 Culture medium (composition: peptone 3g, yeast extract 1g, glucose 6g, cholesterol 50mg, calcium chloride 220mg, corn oil 20g, add water to 1L), adjust the pH value to 7.0~10.0, add agar powder 8g, dissolve, cool to 40 ℃, add Pythium sp.GY1938 spores to a quantity of 1×10 11 Homogenize and stir to obtain a gel suspension, put it in a mold immediately, cut into pieces to shape after solidification, and package and store at 4-8°C.

Embodiment 3

[0035] Embodiment 3 Preparation of mosquito-killing gel of the present invention

[0036] Take 1 / 2KPYG 2 Culture medium (composition: 0.75g peptone, 0.25g yeast extract, 1.5g glucose, 12.5mg cholesterol, 55mg calcium chloride, 5g corn oil, add water to 1L), add 25g trehalose, 30g corn oil, OP-101g, adjust When the pH value reaches 7.0~10.0, add 2g of agar powder, dissolve, cool to 60°C, add Pythium sp.GY1938 spores until the number is 1×10 10 Homogenize and stir to obtain a gel suspension, put it in a mold immediately, cut into pieces to shape after solidification, and package and store at 4-8°C.

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Abstract

The invention discloses a mosquito-killing gel. The gel is prepared by taking Pythium sp.GY1938 swarm spore as an active ingredient and adding auxiliary materials. The gel comprises the following components: 1*10<10>10<11> Pythium sp.GY1938 swarm spores / L and 1-8g / L of 1 / 4-2* common fungus culture medium and coagulating agent. The invention further discloses a preparation method of the mosquito-killing gel. The mosquito-killing gel disclosed by the invention has the advantages of excellent mosquito-killing effect, high stability, simple preparation method and low cost.

Description

technical field [0001] The invention relates to a mosquito-killing gel. Background technique [0002] Mosquitoes are Diptera insects, which can transmit malaria, filariasis, yellow fever and dengue fever and other mosquito-borne diseases, threatening human health. Their development is metamorphosis, and they need to go through eggs, larvae (larvae), pupae, and adults4 period. Compared with adults, mosquito larvae must live in water at a high density, so the aquatic stage is the most effective entry point for mass killing mosquitoes and controlling mosquito-borne diseases from the source. At present, the common methods of mosquito control are still traditional chemical pesticides, but chemical pesticides pollute the environment, disrupt the ecological balance, and cause insecticide resistance. Biological control is the use of natural enemies of pests or microorganisms that can cause diseases to pests to control or kill pests. It is highly specific to pests, non-toxic and h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N25/04A01P7/04
Inventor 杨平李敏惠潘克俭苏晓庆黄盛文李华邹强王玉明王伦安
Owner CHENGDU MEDICAL COLLEGE
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