Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Special primer, kit and method for testing miRNA-155 in bladder cancer urine

A technique for detecting miRNA-155 and primers, applied in DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as the influence of subjective factors, the limitation of bladder cancer stage prediction, etc., and achieve lower detection costs, reliable result analysis, and simplified operations The effect of steps

Active Publication Date: 2013-12-11
SHANDONG UNIV QILU HOSPITAL
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cystoscopy is a common method for the diagnosis of bladder cancer, which can cause complications such as urethral and bladder damage and infection in varying degrees; urine exfoliation cytology has the advantages of non-invasiveness and high specificity, and its sensitivity is less than 50%. Only 30% of the patients are affected by subjective factors of the tester; CT and ultrasonography are currently the most commonly used imaging methods for the diagnosis of bladder cancer and preoperative staging, but it is difficult to find small lesions in the bladder. Forecasts are limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Special primer, kit and method for testing miRNA-155 in bladder cancer urine
  • Special primer, kit and method for testing miRNA-155 in bladder cancer urine
  • Special primer, kit and method for testing miRNA-155 in bladder cancer urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Composition and preparation of the kit:

[0045] According to the nucleotide sequences miRNA-155 (MIMAT0000646), miRNA-191 (MIMAT0000440), miRNA-16 (MIMAT0000069) reported in the microRNA database (http: / / www.mirbase.org / ) as templates, self-designed detection target genes (miRNA-155) and internal reference genes (miRNA-191 and miRNA-16), the primer sequences and Tm values ​​are shown in Table 1.

[0046] Table 1 Primer sequence, Tm value

[0047]

[0048]

[0049] The special kit also includes an RNA isolation solution; the RNA isolation solution consists of 2.5% (volume percentage) Tween 20, 50mmol / L tris, 1mmol / L ethylenediaminetetraacetic acid, 1% (mass percentage) Composed of bovine serum albumin and water.

[0050] The special kit also includes PCR reaction solution: composed of 1×Syber Green I fluorescent dye, DNA polymerase, dNTPs, tris hydrochloride, potassium chloride, magnesium chloride and water.

[0051] The concentration of each substance was ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a special primer, a kit and a method for testing the miRNA-155 in bladder cancer urine, so that the expression amount of the miRNA-155 in bladder cancer urine can be rapidly, conveniently and accurately tested. The special kit comprises a special reverse transcription primer and a test primer for testing the miRNA-155 in bladder cancer urine and a RNA (ribonucleic acid) separation solution; the RNA separation solution is composed of 2.5% of Twain 20, 50mmol / L of tri-hydroxymethyl aminomethane, 1mmol / L of ethylenediamine tetraacetic acid and 1% of serum albumin. According to the special primer, the kit and the method for testing the miRNA-155 in bladder cancer urine, important references are provided for early discovery and early treatment of bladder cancer.

Description

technical field [0001] The invention relates to a special primer and a kit for detecting bladder cancer urine miRNA-155, and a method for detecting the expression level of bladder cancer urine miRNA-155, belonging to the technical field of molecular biology detection. Background technique [0002] Bladder cancer is the most common malignant tumor in the genitourinary system, and its morbidity and mortality both rank first in male genitourinary system tumors in my country, and it shows an increasing trend year by year. 30% of bladder cancer patients have muscle invasion when they are first diagnosed, while 30% of non-muscle invasive bladder cancer patients will relapse into invasive bladder cancer. Early diagnosis and early treatment are the key to prevention and control of bladder cancer. Currently, clinical diagnosis of bladder cancer mainly relies on cystoscopy, urine exfoliative cytology and imaging examinations. Cystoscopy is a common method for the diagnosis of bladde...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
Inventor 王传新张欣刘益民
Owner SHANDONG UNIV QILU HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com