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Stem cells or other bioactive substances doped surgical dressing

A wound dressing and combined technology, applied in the field of clinical care, can solve the problems of short duration, insignificant effect, long onset time, etc., and achieve the effects of reduced time, reduced surgical procedure cost, and short onset time

Inactive Publication Date: 2013-05-08
北京清美联创干细胞科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the traditional single chitosan dressing with long onset time and insignificant effect, and single chitosan oligosaccharide with obvious effect, but rapid degradation, short time effect, frequent dressing changes, etc., and provides a long-acting combined dressing Wound dressing and its preparation method, and adding mesenchymal stem cells and cytokines in the preparation process

Method used

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  • Stem cells or other bioactive substances doped surgical dressing
  • Stem cells or other bioactive substances doped surgical dressing
  • Stem cells or other bioactive substances doped surgical dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Accurately weigh 0.5 g of chitosan and place it in a stirrer, add 100 ml of purified water, dropwise add 0.4 ml of glacial acetic acid, stir well at a stirring speed of 65 r / min, until completely dissolved and form a uniform solution. Add 6 grams of gelatin to the solution, heat it on a water bath at 75° C. until the gelatin is completely dissolved, then add 1.5 grams of chitosan and 10 grams of chitosan and stir to dissolve it. The stirring speed is 150 r / min. Add 1 ml of glycerin, stir until it is completely dissolved, the stirring speed is 100r / min, control the pH to 6.5, then filter to remove impurities, let stand to remove bubbles, subpackage and sterilize, and finally add 10 8 / ml of mesenchymal stem cells and FGF, stored at 4°C, is the finished product.

Embodiment 2

[0021] Example 2: Accurately weigh 2 grams of chitosan and put it in a stirrer, add 100 milliliters of purified water, dropwise add 1 milliliter of glacial acetic acid, stir well, and stir at a speed of 150 r / min until completely dissolved to form a uniform solution. Add 4 grams of gelatin to the solution, heat it on a water bath at 75° C. until the gelatin is completely dissolved, then add 4 grams of chitosan and 6 grams of chitosan and stir to dissolve it. The stirring speed is 150 r / min. Then add 4 ml of glycerin, stir until completely dissolved, the stirring speed is 100r / min, control the pH to 5.5, then filter to remove impurities, let stand to remove bubbles, sub-package and sterilize, and finally add 10 7 / ml of mesenchymal stem cells and FGF, stored at 4°C, is the finished product.

Embodiment 3

[0022] Example 3: Accurately weigh 4 grams of chitosan and put it in a stirrer, add 100 milliliters of purified water, dropwise add 3 milliliters of glacial acetic acid, stir well, and stir at a speed of 200 r / min until completely dissolved to form a uniform solution. Add 2 grams of gelatin to the solution, heat it on a water bath at 75° C. until the gelatin is completely dissolved, then add 6 grams of chitosan and 2 grams of chitosan and stir to dissolve it. The stirring speed is 150 r / min. Then add 6 ml of glycerin, stir until it is completely dissolved, the stirring speed is 100r / min, control the pH to 5, then filter to remove impurities, let stand to remove bubbles, sub-package and sterilize, and finally add 10 6 / ml of mesenchymal stem cells and FGF, stored at 4°C, is the finished product. .

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Abstract

The invention provides a method for fixing bioactive molecules, stem cells and other medicinal materials having treatment values in a surgical dressing. The bioactive substances are integrated and doped into the surgical dressing, so the method is particularly useful in the development of a novel dressing material system, and human mesenchymal stem cells and bioactive substances extracted from a patient (or allogeneic equivalent thereof) can be used in the wound or target operation position through the novel system by a medical staff in order to make the wound rapidly and effectively heal. The wound dressing comprises chitosans, chitosan oligomers, chitosan oligosaccharides, acetate acid gracial, gelatin, glycerin, purified water, mesenchymal stem cells and fibroblast growth factors.

Description

technical field [0001] The invention relates to the field of clinical nursing, in particular to a surgical dressing mixed with stem cells and chitosan. Background technique [0002] The term "stem cells" refers to a class of undifferentiated cells that possess the ability to self-renew while retaining the distinct potential to form differentiated cells and tissues. Stem cells can be totipotent, pluripotent or multipotent. There are also derived stem cells that have lost the ability to differentiate, which are referred to as "nullipotent" stem cells. Totipotent stem cells are cells capable of forming all cells and tissues found in an intact organism, including extraembryonic tissue (ie, the placenta). Totipotent cells include very early embryos (8 cells) and have the ability to form complete organisms. A pluripotent stem cell (pluripotent stem cell) is a cell capable of forming all tissues found in a complete organism, although a pluripotent stem cell cannot form a complet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/28A61L15/20A61L15/44A61L15/40
Inventor 田杰
Owner 北京清美联创干细胞科技有限公司
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