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Method for extracting holothuria leucospilota glycosaminoglycans

A technology of yuzu sea cucumber and glycosaminoglycans, which is applied in the field of extraction of natural active substances in biotechnology, can solve problems such as inability to inject, and achieve the effect of improving purity

Inactive Publication Date: 2013-05-08
QINGDAO MUNICIPAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glycosaminoglycans are the main medicinal ingredients in sea cucumbers related to the treatment of stroke; however, the glycosaminoglycans of sea cucumbers extracted from sea cucumber yuzu are crude products (mass purity is only 50%~60%), and cannot be directly used to make injections

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0013] The method for extracting glycosaminoglycans from sea cucumber yuzu in this embodiment includes the following steps: step 1: pretreatment of sea cucumber yuzu; step 2: alkaline hydrolysis of the body wall of sea cucumber yuzu; step 3: double-enzyme hydrolysis of the body wall of sea cucumber yuzu; Step 4: After the enzymatic hydrolysis is completed, use trichloroacetic acid to remove the protein in the double-enzyme hydrolysis solution; Step 5: Precipitate the glycosaminoglycans of sea cucumber yuzu with ethanol; Step 6: dry the glycosaminoglycans of sea cucumber yuzu; Seventh: purifying glycosaminoglycans of sea cucumber yuzu by cellulose ion exchange column chromatography; step 8: drying glycosaminoglycans of sea cucumber yuzu as refined sugar. The specific method of each step is:

[0014] Step 1: Pretreatment of yuzu sea cucumber; quickly rinse the yuzu sea cucumber with distilled water to remove the foreign matter adhered to the surface of the yuzu sea cucumber, use...

Embodiment 2

[0026] The method for extracting glycosaminoglycans from sea cucumber yuzu in this embodiment includes the following steps: step 1: pretreatment of sea cucumber yuzu; step 2: alkaline hydrolysis of the body wall of sea cucumber yuzu; step 3: double-enzyme hydrolysis of the body wall of sea cucumber yuzu; Step 4: After the enzymatic hydrolysis is completed, use trichloroacetic acid to remove the protein in the double-enzyme hydrolysis solution; Step 5: Precipitate the glycosaminoglycans of sea cucumber yuzu with ethanol; Step 6: dry the glycosaminoglycans of sea cucumber yuzu; Seventh: purifying glycosaminoglycans of sea cucumber yuzu by cellulose ion exchange column chromatography; step 8: drying glycosaminoglycans of sea cucumber yuzu as refined sugar. The specific method of each step is:

[0027] Step 1: Pretreatment of yuzu sea cucumber; quickly rinse the yuzu sea cucumber with distilled water to remove the foreign matter adhered to the surface of the yuzu sea cucumber, use...

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Abstract

The invention belongs to the field of extracting a natural active substance through a biotechnology, and in particular relates to a method for extracting holothuria leucospilota glycosaminoglycans. The method comprises the following steps of, 1, pretreating holothuria leucospilota; 2, performing alkaline hydrolysis on the body wall of the holothuria leucospilota; 3, performing bienzyme enzymolysis on the body wall of the holothuria leucospilota; 4, after finishing the enzymolysis, removing protein in a bienzyme enzymolysis solution by using trichloroacetic acid; 5, depositing the holothuria leucospilota glycosaminoglycans by using ethanol; 6, drying crude holothuria leucospilota glycosaminoglycans; 7, purifying the holothuria leucospilota glycosaminoglycans by using a cellulose ion exchange column chromatography; and 8, drying the holothuria leucospilota glycosaminoglycans. By adopting the method, the relationship between the yield and the purity of the holothuria leucospilota glycosaminoglycans can be balanced, and the purity of the holothuria leucospilota glycosaminoglycans is improved.

Description

technical field [0001] The invention belongs to the field of extracting natural active substances in biotechnology, and in particular relates to a method for extracting glycosaminoglycan from yuzu sea cucumber. Background technique [0002] With the increase of population aging, cardiovascular and cerebrovascular diseases have become the biggest threat to human health. According to reports, cardiovascular and cerebrovascular diseases are one of the three major causes of death for the elderly. At present, the deaths due to cardiovascular and cerebrovascular diseases account for 34% of the total death toll, and about 3 / 4 of the surviving patients have different degrees of labor. Loss of ability and self-care ability. Ischemic stroke is the most common disease in cardiovascular and cerebrovascular diseases, accounting for 43-65% of cerebrovascular diseases. Human survival and quality of life. The world population-standardized incidence rate of stroke is 239.4 / 100,000, of whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 宣世英李雪洁林中华姜曼姜相君辛永宁
Owner QINGDAO MUNICIPAL HOSPITAL
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