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Detection liquid for diagnosing recessive endometritis of dairy cattle

A technology for endometritis and liquid detection, which is applied in the direction of material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, which can solve problems such as delayed treatment time, cumbersome operations, and elimination of cows , to increase economic benefits, increase conception rate, and reduce production costs

Inactive Publication Date: 2013-05-08
张洪涛
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned traditional methods are cumbersome, time-consuming, and uneconomical, especially if the treatment time is delayed, resulting in the transformation of the disease into intractable inflammation and the elimination of dairy cows

Method used

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  • Detection liquid for diagnosing recessive endometritis of dairy cattle
  • Detection liquid for diagnosing recessive endometritis of dairy cattle
  • Detection liquid for diagnosing recessive endometritis of dairy cattle

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Embodiment Construction

[0006] Embodiments of the present invention are described in detail below. A detection solution for diagnosing latent endometritis in cows, said detection solution comprising a standard solution and a staining solution; said standard solution consists of 30g sodium dodecylsulfonate, 1000ml distilled water and 0.1g bromocresol violet Composition; during preparation, 30g sodium dodecylsulfonate was dissolved in 1000ml distilled water, the pH value was adjusted to 6.4 with 2M sodium hydroxide, and 0.1g bromocresol violet was added; the staining solution was composed of 1.5% by volume Composition of eosin, 50% methanol, 0.5% glacial acetic acid, 45% distilled water and 3% sodium tetraborate.

[0007] For diagnostic purposes, select dairy cows that have not been mated after delivery, inject 15-30ml of sterile normal saline into the uterine horns, massage the uterus, and then collect the intrauterine fluid; prepare the staining solution, normal saline and intrauterine fluid in equal...

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Abstract

The invention relates to a detection liquid for diagnosing recessive endometritis of dairy cattle and belongs to the detection agents for livestock diseases. The detection liquid comprises a standard liquid and a staining fluid. The standard liquid consists of sodium dodecyl sulfate, distilled water and bromcresol purple, and the PH value is adjusted by sodium hydroxide. The staining fluid consists of eosin, methanol, glacial acetic acid, distilled water and sodium tetraborate which are combined according to volume ratio. The detection liquid can be used to diagnose whether cattle suffer from recessive endometritis or not before clinical symptoms appear. Compared with conventional diagnostic technology, the detection liquid is simple and convenient, economic and applicable, low in cost, quick and higher in accuracy rate of diagnosis.

Description

technical field [0001] The invention belongs to a detection agent for diagnosing animal husbandry diseases, and mainly relates to a detection solution for diagnosing dairy cow recessive endometritis. Background technique [0002] The materials required for the traditional detection method of recessive endometritis in dairy cows include insemination guns and coats, sterile saline, sterile syringes, centrifuge tubes, small centrifuges, pipettes, glass slides, staining solutions, biological Microscope etc. When testing, select dairy cows that have not been mated after delivery, inject 15-30ml of sterile normal saline into the uterine horns, massage the uterus, then collect the intrauterine fluid into a centrifuge tube and bring it back to the laboratory; the processing and staining of the samples refer to Shake the sample well, take 1ml of uterine content and centrifuge, discard the supernatant, take 50 microliters of the precipitate, apply it on a glass slide, dry it, and sta...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 张金友韩志强王金涛张洪涛麻名汉时代勇姜宏星肖培伟胡凯文张明臣张威李雁彬
Owner 张洪涛
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