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Protected alpha-amylase

A kind of amylase, protected technology, applied in the forming or processing of animal feed, edible oil/fat, animal husbandry, etc., can solve the problem of reducing the nutritional potential of feed

Inactive Publication Date: 2013-05-22
KEMIN IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, shortages of endogenous amylases in animals, especially young animals, limit starch utilization and reduce the nutritional potential of the feed

Method used

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  • Protected alpha-amylase
  • Protected alpha-amylase
  • Protected alpha-amylase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The formulation shown in Table 1 was used to prepare the protected α-amylase.

[0030] Table 1. Formulas of protected α-amylases

[0031]

Embodiment 2

[0033] In order to develop an alpha-amylase that bypasses the stomach and evaluate the product, 4 parameters were measured to evaluate the protected amylase: 1) resistance in acidic pH and efficient release in neutral pH tested in vitro, 2) A two-step sugar release assay to mimic digestion in the stomach and small intestine, 3) a three-step sugar release assay to mimic digestion in the mouth and esophagus, stomach and small intestine, and 4) thermostability.

[0034] Materials and methods

[0035] enzyme .Get Refined TM Alpha-amylase (from Bacillus subtilis, available from Jiangmen, China) and BAN 800 TM (from Bacillus amyloliquefaciens, available from Novozymes). Both enzymes are available in powder form.

[0036] Protected α-amylase contains about 30% (w / w) Refined TM Alpha-amylase. Mix alpha-amylase with polymer (including adhesive and lubricant) evenly. All coating materials are in the positive list of feed ingredients published by the Chinese Ministry of Agricultu...

Embodiment 3

[0090] Metabolism assays in mature roosters to study unprotected Effect of α-amylase, gastro-protected α-amylase and their mixtures (in different ratios) on the apparent metabolizable energy (AME) and digestibility of nutrients of corn-soybean based diets.

[0091] A total of 36 finished breeder roosters (finished breeder rooster) from Arbor Acres (AA) of similar weight were randomly assigned to 6 groups, with one chicken for each replicate, and 6 replicates for each treatment. Group A was treated with rice bran (vehicle) and used as blank control. Groups B to F were treated with gastric-protected α-amylase and unprotected α- Amylase mixture treatment. The α-amylase activity of the formulation was 300 U / g, and the application dose was 500 g / ton of finished feed. The experiment lasted for 11 days, including a 7-day pre-experiment period and a 4-day formal experiment period. AME as well as digestibility of dry matter (DDM), digestibility of crude protein (DCP) and digestibi...

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PUM

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Abstract

A protected a-amylase is described. Pelletization and encapsulation technology, in which slow-release materials and pH-sensitive materials are used to protect the a-amylase from inactivation at low pH. The protected a-amylase was found to be highly stable after as long as 3- hour treatment in acid (pH 3.0). When treated in pH 3.0 for 1 hour and followed by treatment with lipase and pancreatin in pH 7.0 for 2 hour to simulate in vivo environment, it was found that a-amylase was fully released and measurable. In addition, the protected a-amylase showed superior efficacy to the unprotected a-amylase. The protected a-amylase also demonstrated enhanced thermostability.

Description

Background technique [0001] This application claims priority to US Patent Application Serial No. 61 / 295,444, filed January 15, 2010, which is incorporated herein by reference in its entirety. [0002] The present invention relates generally to enzymes for improving the digestibility of feedstuffs, and more particularly to alpha-amylases which are protected from inactivation by acid or heat. [0003] Dietary starch, the animal's main source of energy, is degraded to glucose by endogenous amylases in the mouth and small intestine; it is glucose that provides energy to the animal. However, a shortage of endogenous amylases in animals, especially young animals, limits the utilization of starch and reduces the nutritional potential of the feed. Exogenous amylases have been used for decades to improve starch digestibility and improve animal growth performance. [0004] The oral cavity and small intestine are where starch is digested. In a pH-neutral environment, amylase in saliva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23D9/013
CPCA23K1/004A23K1/1826A23K1/184A23K1/1653A23K1/1893A23K40/30A23K20/189A23K50/75A23K50/30A23K50/60
Inventor Z·段Y·刘Y·嫪D·陈J·马
Owner KEMIN IND INC