Superfine crushing wall-breaking method of haematococcus pluvialis cell

A technology of Haematococcus pluvialis and ultrafine pulverization, applied in the direction of microbial dissolution, etc., can solve the problems of low extraction rate of astaxanthin, achieve the effects of preventing high temperature damage, simple operation process, and high degree of mechanization

Inactive Publication Date: 2013-05-29
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the extraction rate of astaxanthin is low after using the existing wall-breaking method to break the wall of Haematococcus pluvialls;

Method used

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  • Superfine crushing wall-breaking method of haematococcus pluvialis cell
  • Superfine crushing wall-breaking method of haematococcus pluvialis cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Take out the Haematococcus pluvialis spore powder stored in the dark; freeze-dry it for 2 hours in the dark at -10°C; weigh 200g, add it into the working chamber through the feeding port, and fill it with - Precooling with liquid nitrogen at 197°C for 2 minutes; adjust the flow rate of liquid nitrogen to 0.2m / s, and continuously feed liquid nitrogen;

[0030] (2) Turn on the superfine pulverization equipment at a speed of 26000r / min. After superfine pulverization for 1min, stop the equipment; collect the broken Haematococcus pluvialis spore powder in the cloth bag at the sample outlet.

[0031] Observation by electron microscope showed that the particle size of the treated sample was significantly smaller than that before ultrafine crushing, and a large number of cell wall fragments appeared in the sample. Among them, 78.6% of the cells of Haematococcus pluvialis had cell wall defects; 19.3% of the cells of Haematococcus pluvialls had cracks in the cell walls; the t...

Embodiment 2

[0034] (1) Take out the Haematococcus pluvialis spore powder stored in the dark; freeze-dry it for 2 hours at -15°C in the dark; weigh 200g, add it into the working chamber through the feeding port, and fill it with - Precooling with liquid nitrogen at 197°C for 2 minutes; adjust the flow rate of liquid nitrogen to 0.4m / s, and continuously feed liquid nitrogen;

[0035] (2) Turn on the superfine pulverization equipment at a speed of 26000r / min. After superfine pulverization for 1min, stop the equipment; collect the broken Haematococcus pluvialis spore powder in the cloth bag at the sample outlet.

[0036] Observation by electron microscope showed that the particle size of the treated sample was significantly smaller than that before ultrafine crushing, and a large number of cell wall fragments appeared in the sample. Among them, 84.7% of the cells of Haematococcus pluvialis had defects in their cell walls; 14.1% of the cells of Haematococcus pluvialls had cracks in their cell ...

Embodiment 3

[0039] (1) Take out the Haematococcus pluvialis spore powder stored in the dark; freeze-dry it for 2 hours at -15°C in the dark; weigh 200g, add it into the working chamber through the feeding port, and fill it with - Pre-cooling with liquid nitrogen at 197°C for 2 minutes; adjust the flow rate of liquid nitrogen to 0.4m / s, and continuously feed liquid nitrogen;

[0040] (2) Turn on the superfine pulverization equipment at a speed of 26000r / min. After superfine pulverization for 1min, stop the equipment; collect the broken Haematococcus pluvialis spore powder in the cloth bag at the sample outlet.

[0041] The particle size of the treated sample was significantly smaller than that before ultrafine crushing, and a large number of cell wall fragments appeared in the sample. Among them, 83.3% of the cells of Haematococcus pluvialis had cell wall defects; 15.2% of the cells of Haematococcus pluvialls had cracks in the cell walls; the total broken rate was 98.5%.

[0042] The abso...

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Abstract

The invention relates to a wall-breaking method of algae, and in particular relates to a superfine crushing wall-breaking method of a haematococcus pluvialis cell. According to the method, superfine crushing equipment is adopted and is matched with liquid nitrogen equipment, so that the haematococcus pluvialis is frozen instantaneously to become brittle. Therefore, the wall-breaking rate is improved, and the protection environment of a low temperature and oxygen isolation are realized. After the wall-breaking treatment is carried out on the haematococcus pluvialis cell by using the method, the active substance of the haematococcus pluvialis cell has a high leaching rate and is not oxidated, so that the extraction ratio of the active substance is improved. The method is simple in process and convenient to operate, and can be used for performing the one-step treatment on a small quantity of samples in a short time and can also be matched with continuous equipment, sample collection equipment and the like to realize the automatic and continuous production, i.e., the mechanization degree is higher, so that the method is suitable for industrialized production. In addition, the samples are not polluted during the equipment running process, so that the safety is high.

Description

technical field [0001] The invention relates to a method for breaking algae walls, in particular to a method for ultrafine grinding and breaking walls of Haematococcus pluvialis cells. Background technique [0002] Haematococcus pluvialis ( Haematoccoccus pluvialis ) is a widely distributed unicellular green algae, which can form chlamydospores and accumulate a variety of carotenoids with physiological functions in large quantities under stress conditions: astaxanthin, α-carotene, β-carotene, lutein Cryptoxanthin, zeaxanthin, canthaxanthin, etc., 80% of which are astaxanthin and its esters. Haematococcus pluvialis is currently known as the biological resource with the highest content of natural astaxanthin, October 2010 , the Ministry of Health approved Haematococcus pluvialis as a new resource food, and the use of Haematococcus pluvialis to produce carotene active ingredients has broad prospects. For the passage of ingredients such as anthaxanthin, only some techniques ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06
Inventor 赵晓燕陈相艳崔莉陈锋亮王宪昌陈军邓鹏
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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