Skeletal muscle whole organ acellular matrix, its preparation method and its derived medical products

Abstract

The invention discloses a skeletal muscle whole organ acellular matrix, its derived medical products, such as particles, fluidized compositions, gels and active peptides, and preparation and use methods of the matrix and the medical products. The skeletal muscle whole organ acellular matrix completely reserves the skeletal muscle three-dimensional parallelly-arrayed skeletal muscle basement membrane ultra-structure, the vascular matrix network and muscle-tendon joints, reserves large amounts of bioactive components comprising growth factors, hyaluronic acid, glycosaminoglycan, laminins and the like, is highly affinity to muscle-derived stem cells, has the advantages of certain mechanical strength and toughness, thorough decellularization, no obvious immunological rejection, and no ethical or moral issues, and is a skeletal muscle regeneration scaffold and platform having the most advantages reported so far. The prepared skeletal muscle whole organ acellular matrix and its derived medical products can be used for the in-vitro and in-vivo construction and regeneration of the skeletal muscles, are suitable for restoring the skeletal muscle coloboma of various positions in various ranges, and are expected to solve the large-range skeletal muscle coloboma restoration and muscle regeneration problems.

Description

technical field [0001] The present invention describes the preparation method and application of skeletal muscle whole organ acellular matrix (Whole organ cellular matrix, WOACM) and derived medical products such as microparticles, fluidized compositions, gels and active peptides. Areas involved include tissue engineering and regenerative medicine, surgery, and biomaterials. Background technique [0002] The treatment of skeletal muscle defects, especially the functional reconstruction of large-scale muscle defects, is still a clinical problem so far. Skeletal muscles make up 40-50% of the body weight. Skeletal musculoskeletal trauma is also one of the most common traumas in the human body. According to statistics, 30-55% of sports injuries and 50-70% of war injuries involve skeletal muscles. For minor skeletal muscle injuries, such as various contusions or strains, skeletal muscle tissue can rely on myoblasts (satellite cells) to regenerate muscle fibers to achieve self-r...

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Problems solved by technology

However, it is worth noting that this method still has some limitations, including (1) complicated operation. The ECM materials used in the above research are UBM and SIS. Although the source is convenient, because the average thickness of UBM and SIS is less than 100 μm, it is necessary to To reach the volume of the defective skeletal muscle, hundreds of layers of materials need to be laminated; ②The regenerated skeletal muscle is not a skeletal muscle with full contractile function in the true sense: the substance metabolism of the skeletal muscle is very strong, and the blood flow in the skeletal muscle can reach It is 20-80 times that of the resting state, so the normal human skeletal muscle has relatively large-diameter innervating blood vessels, and there are 1350-3000 capillaries in each square millimeter of muscle; while the blood supply of regenerated skeletal muscle in UBM and SIS implantation areas is the same. Provided by capillaries invading from surrounding tissues, there are no obvious innervating blood vessels, and the density of capillaries is insufficient to provide the blood flow required for severe contraction; ③UBM and SIS fail to reflect the complex structure of the natural skeletal muscle matrix and regenerate the muscle fibers of skeletal muscle Unsatisfactory arrangement

In short, ECM-induced functional regeneration of skeletal muscle is a very promising technology, but there are still places worthy of further study for improvement.

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