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Preparation method of nano fiber double layer support used for skin tissue engineering

A nanofiber and double-layer technology is applied in the field of preparation of nanofiber double-layer scaffolds, which can solve problems such as singleness and achieve the effect of simple and feasible preparation method.

Active Publication Date: 2014-09-10
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the double-layer scaffolds used in the current literature and published patents are microporous structures, and are prepared by a single method.

Method used

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  • Preparation method of nano fiber double layer support used for skin tissue engineering

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Embodiment Construction

[0016] The method of the present invention will be further described below in conjunction with specific embodiments, but the implementation of the present invention is not limited thereto.

[0017] Weigh 0.6 g of polylactic acid and dissolve it in 10 ml of tetrahydrofuran at 50 °C. Pour the solution into a weighing bottle and place it in a -18°C freezer overnight to form a polylactic acid gel. The gel was replaced with water at 4°C for 2 days, and the water was changed 3 times a day to remove the tetrahydrofuran solvent. After water replacement, the polylactic acid gel was frozen again at -18°C for 12 hours, and then placed in a vacuum dryer to freeze-dry for 3 days. After taking it out, cut it into a nano-polylactic acid sheet with a thickness of about 1 mm, and stick the sheet on the aluminum foil with conductive double-sided adhesive tape. Weigh 0.7 g of chitosan powder and add 10 ml of trifluoroacetic acid. Under the condition of reflux at 70 ℃, chitosan was dissolved. ...

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Abstract

The invention relates to a preparation method of a nano fiber double layer support used for skin tissue engineering, which belongs to the biology medical material technical field. Polylactic acid, chitosan and polycaprolactone are taken as the support materials, a thermotropic phase separating technology and a high pressure static spinning technology are combined to prepare the porous double layer network support having a nano fiber structure. The bottom layer of the double layer support is an polylactic acid nano fiber support prepared by the thermotropic phase separating technology, fiber diameter is between 200-750nm, the surface layer is the chitosan-polycaprolactone composite nano fiber film prepared by the static spinning technology, and the fiber diameter is about 200nm. The support stimulates a cuticle and corium double layer structure of human body skin from a microstructure, and is suitable for restoration of the human body skin.

Description

technical field [0001] The invention relates to a method for preparing a nanofiber double-layer scaffold for skin tissue engineering, and belongs to the technical field of biomedical materials. Background technique [0002] The main purpose of the research and preparation of biomedical materials is to use various natural or synthetic materials to make artificial organs or medical devices to replace and repair damaged tissues and organs of the human body, and finally realize the normal physiological functions of tissues and organs. [0003] As the largest organ of the human body, the skin plays a role in protecting the human body from the invasion of external objects. Damage to the skin can lead to the invasion of bacteria and pathogens, and severe cases can lead to death. Therefore, in the past three decades, a large number of studies on skin scaffolds have been carried out in the fields of biomedical materials and tissue engineering. So far, a variety of commercial produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/20A61L27/18
Inventor 汪学军楼涛
Owner QINGDAO UNIV