Application of triacetyl glucal in preparation of antiviral drug
A technology of antiviral drugs and glucal sugar, which is applied in antiviral agents, pharmaceutical formulas, medical preparations containing active ingredients, etc.
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Embodiment 1
[0020] Effects of triacetylglucenose on diabetic mice induced by alloxan
[0021] Test protocol: Take Kunming mice, fast for 12 hours, inject 80mg / kg of alloxan into the tail vein, measure blood glucose after 3 days, take blood glucose above 11.2-22.4mmol / L and randomly divide them into model control group and triacetylglucene In the sugar group, another 10 rats were taken as the normal control group, and the normal saline was injected into the tail vein at 0.2 ral / mouse. There were 10 rats in each group. The triacetyl glucenose group was given intragastrically, once a day, with doses of 125 and 250 mg / kg body weight respectively; the model control group and the normal control group were given sterilized distilled water. After 14 days of continuous administration, blood was collected from the tail vein for blood glucose measurement, and the weight of the mice, drinking water and feed consumption on the day of blood collection were recorded.
[0022] The effect of triacetylglucenos...
Embodiment 2
[0031] Antiviral activity of triacetyl glucenose
[0032] Strains: Dog kidney cells (MDCK) and parainfluenza virus type 1 (HVJ) cells and toxicity test to MDCK cells
[0033] The triacetyl glucenose was prepared with distilled water into a 20 mg / mL medicinal solution, filtered and sterilized, and diluted with cell maintenance solution to different concentrations. Digest the MDCK cells in the flask, inoculate them in a 96-well cell culture plate, culture for 2 days in a 37°C, 5% CO2 incubator, remove the original culture medium after growing into a monolayer of cells, and add 100 μL of the medicinal solution of different dilution concentrations, and Set up a cell control with only 100μL of maintenance solution, set 4 replicate holes for each concentration, and place them in the incubator to continue culturing. Observe the cytopathic effect (CPE) of the drug with an inverted microscope every day, with "-" indicating 0% CPE , "+" means 1% to 25% CPE, ++ means 25% to 50% CPE, +++ mean...
Embodiment 3
[0040] In Vitro Screening Test of Anti-tumor Biological Activity of Triacetyl Glucenose
[0041] Screening method: Tetrazolium salt (Methyl-Thiazol-Tetrozlium, MTT reduction method)
[0042] Sulforhodamine B (sulforhodamine B, SRB) protein beam color method.
[0043] Cell lines: BEL-7402 human liver cancer, P338 mouse leukemia, A-549 human lung adenocarcinoma
[0044] Action time: 72 hours
[0045] Result evaluation: invalid: 10 -5 mol / L<85%;
[0046] Weak effect: 10 -5 mol / L≥85% or 10 -6 mol / L>50%;
[0047] Powerful: 10 -6 mol / L≥85% or 10 -7 mol / L>50%.
[0048] The result of the table below shows the inhibitory rate of triacetyl glucenose on tumor cell growth%
[0049] Note: The administration group in the table below is the administration of triacetyl glucenose.
[0050]
[0051]
[0052]
[0053] According to the above test results, triacetylglucenose has strong effects on BEL-7402 human liver cancer, P338 mouse leukemia, and A-549 human lung adenocarcinoma.
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