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Application of bacteroides fragilis in preparing composition for preventing and treating colon cancer

A technology of Bacteroides fragilis and a composition is applied in the application field of Bacteroides fragilis in the preparation of compositions for preventing and treating colon cancer, and achieves the effects of promoting apoptosis, inhibiting tumor growth and having strong pharmacological effects.

Inactive Publication Date: 2013-06-12
广州知光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But Bacteroides fragilis is used as a kind of probiotics, and the relevant application in the preparation and prevention and treatment of colon cancer compositions has not yet been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of Bacteroides fragilis bacterial liquid

[0018] (1) Isolation of Bacteroides fragilis from infant feces

[0019] Weigh 0.5 g of stool according to the "normal flora test method", put it in a test tube filled with 4.5 ml of normal saline, and serially dilute it to 10 -1 ~10 -6 , from high concentration to low concentration on the Bd medium plate, placed in an anaerobic gas box and anaerobic gas bag for anaerobic culture. Store at 37°C, open the can for inspection after 2-7 days. A single colony was selected for pure culture, staining microscopy, culture characteristics, and physiological and biochemical testing.

[0020] (2) Enrichment culture of Bacteroides fragilis and preparation of bacterial solution

[0021] Select the purified single colony and inoculate it in the improved tryptone broth for enrichment culture. The obtained bacterial solution is centrifuged and precipitated at a speed of 3000r / min for 15 minutes, and the supernatant is...

Embodiment 2

[0022] Example 2 Toxicity test of Bacteroides fragilis

[0023] The Bacteroides fragilis bacterium liquid that adopts embodiment 1 preparation carries out following experiment:

[0024] (1) Acute toxicity test in mice

[0025] Sixty C57BR / C mice weighing 22±2 g (half male and half male) were divided into high-dose group, low-dose group and control group, 20 in each group. Each mouse in the high-dose group was intragastrically administered 10 12 cfu / ml bacterial solution 0.5ml, the low dose group was administered orally 10 times a day 9 cfu / ml bacterial solution 0.5ml, and the control group was fed with 0.5ml normal saline every day. Continuous observation for 14 days.

[0026] Experimental results: The mice grew normally, no death was observed, and the LD50 was undetectable.

[0027] (2) Rat toxicity test

[0028] Take 30 SD rats (half male and half female) of 150±20 g, and divide them into 3 groups: high and low dose group and control group. Each rat in the high-do...

Embodiment 3

[0033] Example 3 Preventive effect of Bacteroides fragilis on colon cancer

[0034] The Bacteroides fragilis bacterium liquid that adopts embodiment 1 preparation carries out following experiment:

[0035] Animal model establishment: 60 ICR mice were randomly divided into two groups, namely: normal saline group (10 mice) and model group (50 mice). Dissolve 1,2-dimethylhydrazine (DMH) in physiological saline to a final concentration of 20 mg / kg, adjust the pH to 6.5 with 0.25 mol / L NaOH, and inject intraperitoneally into mice. One week later, dextranside sodium (DSS) was added to the drinking water of the model group at a concentration of 20 g / L, and given to the mice for 7 consecutive days. When the mice had bloody stool for the second time, and at the 9th week after giving DMH, the model mice were randomly divided into 4 groups according to body weight, namely: model group (20 mice) and Bacteroides fragilis group: high-dose group ( 10 9 cfu / ml), middle dose group (10 8 ...

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PUM

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Abstract

The invention relates to the technical field of application of bacteroides fragilis, and in particular relates to application of bacteroides fragilis in preparing a composition for preventing and treating colon cancer. Experiments show that bacteroides fragilis induces an organism to generate the anti-tumor effect in vivo, inhibit the tumor growth, accelerate tumor cell apoptosis and prolong the lifetime, so that bacteroides fragilis has a good effect in preventing and treating colon cancer. The invention explores novel use of bacteroides fragilis and develops a novel application field, thereby indicating that the bacteroides fragilis has good edible and officinal prospect. Bacteroides fragilis serving as probiotic can be used for preparing foods or medical compositions for preventing and treating colon cancer so as to provide a clinically health-care and preventing and treating food suitable for human to take.

Description

technical field [0001] The invention relates to the technical field of application of Bacteroides fragilis, in particular to the application of Bacteroides fragilis in preparing a composition for preventing and treating colon cancer. Background technique [0002] Malignant tumors are the most serious diseases that endanger human health. In some European and American countries, cancer mortality ranks second only to cardiovascular diseases. With the population aging in our country, the morbidity and mortality of tumors have increased, and the mortality of malignant tumors in urban residents has ranked first in the cause of death. At the beginning of the 20th century, it was found that Bulgaria had the highest proportion of long-lived elderly in the same region, and the incidence of tumors was relatively low. It turns out that Bulgarians have the habit of drinking yogurt every day. Studies have found that the probiotics contained in Bulgarian yogurt can promote the decomp...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P35/00A23L1/30A23C9/152A23C9/13A23C19/00A23G9/36A23K1/16
Inventor 智发朝白杨刘洋洋
Owner 广州知光生物科技有限公司
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