Method for assistant selection of goose muscle fatty acid performances by utilizing molecular markers
A molecular marker-assisted, fatty acid technology, applied in the field of animal genetic engineering, can solve the problems that the field of molecular marker selection is still blank, and the effective evaluation and selection method for fatty acid traits of goose muscle has not been proposed, so as to accelerate the progress of genetic breeding, shorten the generation interval, Accurate effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] Embodiment 1: (selection of the fatty acid character of Zhedong white goose muscle)
[0038] (1) Goose THRSP according to GenBank No. EU710582.1 α Gene sequence design primers:
[0039] The upstream primer sequence is ASF: 5'-GTGCTGCCTGTACCGTCCAA-3';
[0040] The downstream primer sequence is ASR: 5'-GGGAAGCAGTTACACCATCAGAG-3';
[0041] (2) PCR amplification reaction: Select 150 individuals from the East Zhejiang White Goose population as the reserved species, collect blood from the wing veins of each individual, extract blood genomic DNA according to the conventional phenol-chloroform method, and use the above primers and genomic DNA The template was subjected to PCR amplification reaction, and the reaction system used was 25 μL: 10×PCR Buffer (Mg 2+ plus) 2.5 μL, 2.5 mmol L -1 dNTP 3.0 μL, 10 μmol L -1 Upstream primer 0.4 μL, 10 μmol L -1 Downstream primer 0.4 μL, 50 ng·μL -1 Template DNA 0.5 μL, 5.0U·μL -1 rTaq enzyme 0.25 μL, ddH 2 O 17.95 μL; PCR am...
Embodiment 2
[0049] Embodiment 2: (selection of Lande goose muscle fatty acid character)
[0050] In this example, the PCR amplification reaction in step (2) is: select 200 individuals of the reserved species in the Lande goose population, and the reaction system used is 25 μL: 10×PCR Buffer (Mg 2+ plus) 2.5 μL, 2.5 mmol L -1 dNTP 2.0 μL, 10 μmol L -1 Upstream primer 0.5 μL, 10 μmol L -1 Downstream primer 0.5 μL, 50 ng·μL -1 Template DNA 0.5 μL, 5.0U·μL -1 rTaq enzyme 0.15 μL, ddH 2 O 18.85 μL; PCR amplification reaction condition is 95 o C pre-denaturation 5 min, 95 o C Denaturation 30s, 55 o C annealing 30s, 72 o C extension 30s, 35 cycles, 72 o C extension 10 min, 4 o C finishes reaction; All the other steps (1), (3), (4) and technology are the same as embodiment 1;
[0051] Here we take 10 individuals of the reserved species as an example. The 10 Lande geese were first measured for the relative content of muscle fatty acids in each individual, and then PCR amplified the p...
Embodiment 3
[0056] Embodiment 3: (selection of Jiangshan white goose muscle fatty acid character)
[0057] In this example, the PCR amplification reaction of step (2) is: select 100 individuals of the reserved species from the Jiangshan White Goose population, and the reaction system used is 25 μL: 10×PCR Buffer (Mg 2+ plus) 2.5 μL, 2.5 mmol L -1 dNTP 1.0 μL, 10 μmol L -1 Upstream primer 0.6 μL, 10 μmol L -1 Downstream primer 0.6 μL, 50 ng·μL -1 Template DNA 0.5 μL, 5.0U·μL -1 rTaq enzyme 0.35 μL, ddH 2 O 19.45μL; PCR amplification reaction condition is 95 o C pre-denaturation 5 min, 95 o C Denaturation 30s, 50s o C annealing 30s, 72 o C extension 30s, 38 cycles, 72 o C extension 10 min, 4 o C finishes reaction; All the other steps (1), (3), (4) and technology are the same as embodiment 1;
[0058] Here we take 10 individuals of the reserved species as an example. The 10 Jiangshan white geese first measured the relative content of fatty acids in each individual muscle, and t...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More