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Saccharomyces cerevisiae strain and application thereof in preparing heat-resistant superoxide dismutase (SOD)

A technology for Saccharomyces cerevisiae and strains, which is applied to Saccharomyces cerevisiae strains and the application field in the preparation of thermostable superoxide dismutase, and achieves the effects of low cost, promotion of development and good stability

Active Publication Date: 2014-08-27
浙江奇化美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But wild strains of Saccharomyces cerevisiae itself have not been screened for thermotolerant SOD

Method used

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  • Saccharomyces cerevisiae strain and application thereof in preparing heat-resistant superoxide dismutase (SOD)
  • Saccharomyces cerevisiae strain and application thereof in preparing heat-resistant superoxide dismutase (SOD)
  • Saccharomyces cerevisiae strain and application thereof in preparing heat-resistant superoxide dismutase (SOD)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Isolation, screening and identification of heat-resistant superoxide dismutase Saccharomyces cerevisiae strains

[0035] 1. Isolation and screening of heat-resistant superoxide dismutase Saccharomyces cerevisiae strain TY-SC-2-2013.1.1

[0036] 1. Isolation of heat-resistant superoxide dismutase Saccharomyces cerevisiae strain

[0037] 1) Soil collection and treatment

[0038] A total of 18 samples of distiller's yeast, cellar mud and fermented grains were collected from Baoding distillery in Hebei Province, placed in sterile paper bags, and marked with the place and date of collection. After transporting it back to the laboratory, store it in the refrigerator for future use.

[0039] The collected sample was obtained by quartering method to obtain 20-30g sample, which was placed in a mortar and ground evenly.

[0040] 2) Separation of strains:

[0041] Weigh 1g of the collected samples respectively, add them into a sterilized Erlenmeyer flask containing ...

Embodiment 2

[0098] The TY-SC-2-2013.1.1 strain, classified as Saccharomyces cerevisiae, was deposited in the General Microorganism Center of China Committee for the Collection of Microbial Cultures (CGMCC for short) on February 1, 2013, and the address is: Beijing No. 3, No. 1 Yard, Beichen West Road, Chaoyang District), the strain preservation number is: CGMCC No.7249. Example 2, application of heat-resistant superoxide dismutase Saccharomyces cerevisiae strain TY-SC-2-2013.1.1

[0099] 1. Fermentation condition research single factor experiment

[0100] 1. Fermentation

[0101] The impact tests of different carbon sources on the biomass of strain cells and the production of SOD were carried out respectively:

[0102] A. The reagents are as follows: (1), yeast medium without carbon source; (2), different carbon sources including glucose, maltose, raffinose, sucrose and starch.

[0103] Remarks: All the above medium components can be obtained from commercial sources.

[0104] B. Ferme...

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Abstract

The invention discloses a Saccharomyces cerevisiae strain and application thereof in preparing heat-resistant superoxide dismutase (SOD). The collection number of the Saccharomyces cerevisiae strain TY-SC-2-2013.1.1 disclosed by the invention is CGMCC No.7249. The invention also provides a method for preparing the SOD, which comprises the following steps: fermenting the Saccharomyces cerevisiae strain TY-SC-2-2013.1.1, and collecting the fermentation product to obtain the SOD. The experiment proves that the screened Saccharomyces cerevisiae can directly express the food-level heat-resistant SOD product, and the high-temperature-resistant microzyme can reduce the trouble and cost due to cooling in the fermentation production process.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Saccharomyces cerevisiae and its application in preparing heat-resistant superoxide dismutase. Background technique [0002] Superoxide dismutase (superoxide dismutase, SOD) widely exists in various organisms, is a natural scavenger of superoxide anion free radicals in the body, and protects the cells of the body. Therefore, SOD plays an important physiological role in anti-aging, tumor prevention and anti-inflammation, and has received great attention from domestic and foreign medicine, daily chemical, food and biochemical circles. [0003] According to the different metal prosthetic groups contained in SOD, it can generally be divided into three types: Cu, Zn-SOD, Mn-SOD and Fe-SOD. [0004] SOD has a wide range of clinical applications, such as osteoarthritis, acute liver and chronic liver inflammation, SOD can remove a large number of superoxide free radicals produce...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12N9/02C12R1/865
Inventor 左德全姜广董亮蒋峰臣
Owner 浙江奇化美生物科技有限公司