Preparation method of multifunctional meso-porous silicon nano preparation carrying gossypol derivative

A nano-preparation, mesoporous silicon technology, applied in the field of preparation of multifunctional mesoporous silicon nano-preparation carrying gossypol derivatives, to achieve good application prospects and economic value, stable fluorescence characteristics and mesoporous, monodispersity Good results

Inactive Publication Date: 2013-06-26
XIDIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no similar report on related patents on traceable drug-loaded composite fluorescent mesoporous silicon nano-preparations

Method used

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  • Preparation method of multifunctional meso-porous silicon nano preparation carrying gossypol derivative
  • Preparation method of multifunctional meso-porous silicon nano preparation carrying gossypol derivative
  • Preparation method of multifunctional meso-porous silicon nano preparation carrying gossypol derivative

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The fluorescent dye Cy5.5 and APTES with a molar ratio of 1:5 were reacted in 11ml of acetone in the dark for 12h to obtain Cy5.5-APTES; 0.5g of CTAB and 20ul of TEA were added to 20ml of aqueous solution, and the set temperature was 30°C, speed 20r / min. When the mixed solution is heated to 30°C, change the rotation speed to 30r / min, stir for 10min, add 0.1ml of TEOS and 30ul of Cy5.5-APTES dropwise to the above mixed solution at a rate of 60 drops per minute, and stir vigorously until A light green sol-like composite fluorescent mesoporous silicon nano-preparation appeared, filtered and dialyzed, and the pH value of the final solution was determined to be 11, and the average particle size was 713d.nm; 0.1 mg of the composite fluorescent mesoporous silicon nanoparticles prepared above was dispersed in In 10ml of ethanol, reflux for 30min, add 0.2ml of a mixture of APTES and diethylaminoethanol with a volume ratio of v / v=1:3, centrifuge and wash to obtain composite fluor...

Embodiment 2

[0035] The fluorescent dye Cy5.5 and APTES with a molar ratio of 1:8 were reacted in 9ml of acetone in the dark for 12h to obtain Cy5.5-APTES; 6g of CTAB and 100ul of TEA were added to 30ml of aqueous solution, and the temperature was set at 40 °C, speed 20r / min. After the mixed solution is heated to 40°C, the speed is changed to 40r / min, and stirred for 20min, 0.2ml of TEOS and 60ul of Cy5.5-APTES are added dropwise to the above mixed solution at a rate of 55 drops per minute, and vigorously stirred until A light green sol-like composite fluorescent mesoporous silicon nano-preparation appeared, filtered and dialyzed, and the pH value of the final solution was determined to be 10, and the average particle size was 403d.nm; 20 mg of the above-prepared composite fluorescent mesoporous silicon nanoparticles were dispersed in 60 ml In ethanol, reflux for 50min, add 10ml of a mixture of aminoacetonitrile hydrochloride and diethylaminoethanol with a volume ratio of v / v=2:5, centrifu...

Embodiment 3

[0037] The fluorescent dye Cy5.5 and APTES with a molar ratio of 1:11 were reacted in 8ml of acetone in the dark for 12h to obtain Cy5.5-APTES; 12g of CTAB and 200ul of TEA were added to 60ml of aqueous solution, and the temperature was set at 50 °C, speed 20r / min. When the mixed solution is heated to 50°C, the rotating speed is changed to 50r / min, and stirred for 40min, 0.4ml of TEOS and 120ul of Cy5.5-APTES are added dropwise to the above mixed solution at a rate of 50 drops per minute, and vigorously stirred until Light green sol-like composite fluorescent mesoporous silicon nanoparticles appeared, filtered and dialyzed, and the pH value of the final solution was determined to be 9, and the average particle size was 381d.nm; 40mg of the above-mentioned composite fluorescent mesoporous silicon nanoparticles were dispersed in 110ml In ethanol, reflux for 70 minutes, add 15ml of a mixture of tetraethyldiaminobenzhydrylamine and aminoacetonitrile hydrochloride with a volume rat...

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Abstract

The invention discloses a preparation method of a multifunctional meso-porous silicon nano preparation carrying a novel gossypol derivative ApoG2. The preparation method comprises the following steps of: adding a defined amount of meso-porous surfactant to a diluted strong base solution, stirring the solution, dropping TEOS (tetraethyl orthosilicate) and cyanine dye Cy5.5 into the solution which is heated to a certain temperature, reacting the solution for a period of time under the conditions of constant temperature and serious stirring, filtering the reaction product, and dialyzing the product for multiple times to prepare fluorescent-dye-doped meso-porous silicon matrix nano particles; and mixing the after centrifuged multifunctional nano particles with a solution of the novel gossypol derivative ApoG2, and oscillating, centrifuging and washing the mixture to obtain the traceable multifunctional meso-porous silicon nano preparation carrying the novel gossypol derivative ApoG2. The preparation method has the advantages of simple procedure, small particle diameter, high monodispersity, stable fluorescence property, high drug loading capacity and the like, thereby enabling the ApoG2 to better play a role in anti-tumor.

Description

technical field [0001] The present invention relates to a preparation process of multifunctional mesoporous silicon nanoparticles loaded with drugs, in particular to traceable multifunctional mesoporous silicon nanoparticles carrying novel gossypol derivatives ApoG2 as fluorescent probes and drug delivery tools in biological Applications in the medical field. Background technique [0002] Mesoporous materials have structural characteristics such as high specific surface area, large pore capacity, and pore diameters between 2.0 and 50.0 nm, which can be adjusted arbitrarily by synthesis conditions, which endow them with many unique properties. They are used in many fields such as heterogeneous catalysis, sensors, adsorption and separation With potential application value, it has become an emerging and flourishing interdisciplinary research field. The unique properties inherent in its nanometer scale have played an active and important role in the non-invasive diagnosis and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K47/04A61K31/122A61K49/00A61P35/00
Inventor 詹勇华孙玉林梁继民田捷张象涵张金瑶
Owner XIDIAN UNIV
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