Method for selectively reducing specific nitrosamines in cigarette smoke

[0024] Example 1

[0025] Dissolve 1 g of chitosan in 50 mL of acetic acid solution with a concentration of 1%, add 50 mL of methanol, and stir to obtain a chitosan solution; dissolve 6 g of β-cyclodextrin in 100 mL of hydrochloric acid solution with a concentration of 0.5 M, add dropwise 10 mL of glutaraldehyde with a concentration of 25% was stirred at 85 °C for 2 h to obtain a cyclodextrin-glutaraldehyde polymer solution; the cyclodextrin-glutaraldehyde solution was added dropwise to the chitosan solution, Under the condition of 85 ℃, the reaction was stirred for 1 h, the pH value was adjusted to 8 with 1M sodium hydroxide solution, the stirring was continued for 10 min, filtered, the filter residue was washed with water, and dried at 40 ℃ to obtain brown-yellow solid particles , is a chitosan-cyclodextrin-glutaraldehyde polymer. Pass through a 40-mesh sieve, weigh quantitatively, and add it to the filter tip at a level of 9 mg per cigarette to make a test cigarette, which is compared with the control cigarette. The control cigarettes were identical to the test cigarettes except that they did not contain additives, such as cigarette materials, sizes, and filter rods. The filter rod is an acetate filter rod.

[0026] The test cigarettes and the control cigarettes were smoked with the RM200 automatic smoking machine under standard smoking conditions, and the smoke test was collected, and then NNN, NNK, NAT, NAB and other compounds were determined according to the relevant requirements of GB/T 23228-2008. The test data are shown in Table 1 below. From the results in Table 1, it can be seen that the chitosan-cyclodextrin-glutaraldehyde polymer can selectively reduce the main tobacco-specific nitrosamines in cigarette smoke. Compared with the control cigarette, the wet tar of the cigarette TPM did not decrease (instead it increased by 4.77%), the decrease rate for NNK was 8.55%, and several other specific nitrosamines also decreased to varying degrees. The overall taste and smoking taste of the cigarettes and the control cigarettes were basically the same.

[0027] Table 1 The effect of chitosan-cyclodextrin-glutaraldehyde polymer in reducing specific nitrosamines

[0028]

[0029] Example 2

[0030]Dissolve 1 g of chitosan in 50 mL of acetic acid solution with a concentration of 1%, add 50 mL of methanol, and stir to obtain a chitosan solution; dissolve 3 g of β-cyclodextrin in 50 mL of hydrochloric acid solution with a concentration of 0.5 M, add dropwise 7mL of glutaraldehyde with a concentration of 25% was stirred at 85°C for 2 hours to obtain a cyclodextrin-glutaraldehyde polymer solution; the cyclodextrin-glutaraldehyde solution was added dropwise to the chitosan solution, Under the condition of 85℃, the reaction was stirred for 1h, the pH value was adjusted to 8 with 1M sodium hydroxide solution, the stirring was continued for 10min, filtered, the filter residue was washed with ethanol, and dried at 40℃ to obtain a brownish yellow solid The particles are chitosan-cyclodextrin-glutaraldehyde polymers. Pass through a 40-mesh sieve, weigh quantitatively, and add 10 mg per cigarette to the filter tip to make a test cigarette, which is compared with a control cigarette. The control cigarettes were identical to the test cigarettes except that they did not contain additives, such as cigarette materials, sizes, and filter rods. The filter rod is an acetate filter rod.

[0031] The test cigarettes and the control cigarettes were smoked with the RM200 automatic smoking machine under standard smoking conditions, and the smoke test was collected, and then NNN, NNK, NAT, NAB and other compounds were determined according to the relevant requirements of GB/T 23228-2008. The test data are shown in Table 2 below. From the results in Table 2, it can be seen that the chitosan-cyclodextrin-glutaraldehyde polymer can selectively reduce the main tobacco-specific nitrosamines in cigarette smoke. Compared with the control cigarette, the wet tar of the cigarette The range of change is only 0.37%, the reduction range of NNK is 3.79%, and several other specific nitrosamines also have different degrees of reduction. The overall taste and taste are basically the same.

[0032] Table 2 The effect of chitosan-cyclodextrin-glutaraldehyde polymer in reducing specific nitrosamines

[0033]

[0034] Example 3

[0035] The chitosan-cyclodextrin-glutaraldehyde polymer prepared in Example 1 was added to the filter tip at a level of 8 mg/cigarette to make a test cigarette, which was compared with a control cigarette. The control cigarettes were identical to the test cigarettes except that they did not contain additives, such as cigarette materials, sizes, and filter rods. The filter rod is an acetate filter rod.

[0036] According to the method of the above example, the results show that the wet tar in cigarette smoke is reduced by 4.43%, while the reduction rates of NNN, NNK, NAT and NAB are 16.26%, 34.54%, 9.89% and 8.21% respectively. The reduction range of nitramine is still greater than that of tar, which achieves the purpose of selective harm reduction.