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Medicinal composition

A kind of composition and medicine technology, applied in the field of medicine

Inactive Publication Date: 2013-07-10
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the research on lutein OC still needs to be improved

Method used

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  • Medicinal composition
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Extracting Garcinia OC from Yunnan Garcinia Cambogia

[0032] Experimental materials: Garcinia cambogia peel was purchased from Luxi City, Dehong Prefecture, Yunnan Province, China in 2006, and the plant samples were preserved in the Innovative Chinese Medicine Laboratory of Shanghai University of Traditional Chinese Medicine.

[0033] Experimental method: Dried and powdered Garcinia cambogia peel (9.0 kg) was extracted back and forth three times with acetone (20 L) at room temperature. The extract was evaporated under reduced pressure to give a dark green residue (1.2 kg). The residue was placed on a silica gel column and eluted successively with chloroform, ethyl acetate and acetone for component separation. The chloroform was partially evaporated in vacuo to give a residue (750 g), of which 400 g was placed on a silica gel column and eluted sequentially with a hexane / acetone gradient system (100:0 to 0:100, v / v) to give four fractions. Fraction II was pl...

Embodiment 2

[0036] Example 2 Lutein OC induces the formation of autophagic vesicles in human cervical cancer cell HeLa

[0037] 1. Experimental materials

[0038] Human cervical cancer cells HeLa were purchased from ATCC in the United States;

[0039] DMEM, trypsin, penicillin and streptomycin, transfection reagent Lipofectamine2000 were purchased from Invitrogen, USA;

[0040] Fetal bovine serum was purchased from PAA, Australia;

[0041] Lutein OC is prepared by Example 1.

[0042] 2. Experimental method

[0043] Use DMEM medium containing 10% fetal bovine serum, 100 U / ml penicillin and 100 μg / ml streptomycin at 37°C, 5% CO 2 Human cervical cancer cell HeLa was cultured in an incubator with saturated humidity, and digested and passaged with 0.25% trypsin, and the cells in the logarithmic growth phase were used for the experiment.

[0044] Human cervical cancer cells HeLa were seeded in 6-well plates (2 × 10 5 cells / well), and after the cell monolayer was confluent, transfected wit...

Embodiment 3

[0049] Garcinia OC induces the formation of autophagic vesicles in human nasopharyngeal carcinoma cell CNE, human colon carcinoma cell HCT116, human breast cancer cell MCF7, human liver carcinoma cell HepG2 and mouse embryonic fibroblast MEF

[0050] 1. Experimental materials

[0051] Human nasopharyngeal carcinoma cell CNE, human colon carcinoma cell HCT116, human breast cancer cell MCF7, human liver cancer cell HepG2 and mouse embryonic fibroblast MEF were purchased from ATCC in the United States;

[0052] DMEM, trypsin, penicillin and streptomycin, transfection reagent Lipofectamine2000 were purchased from Invitrogen, USA;

[0053] Fetal bovine serum was purchased from PAA, Australia;

[0054] Lutein OC is prepared by Example 1.

[0055] 2. Experimental method

[0056] Use DMEM medium containing 10% fetal bovine serum, 100 U / ml penicillin and 100 μg / ml streptomycin at 37°C, 5% CO 2 Human nasopharyngeal carcinoma cell CNE, human colon carcinoma cell HCT116, human breast ...

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Abstract

The invention provides application of morellin OC or pharmaceutically acceptable salts thereof in preparation of a medicament. The medicament is used for treating cancers or inhibiting autophagy.

Description

technical field [0001] The present invention relates to the field of medicine, and more specifically, relates to the use of lutein OC or a pharmaceutically acceptable salt thereof in the preparation of medicines, and the medicines are used for treating cancer or inhibiting cell autophagy. Background technique [0002] There are 21 species of Garcinia L. plants in my country, which are distributed in southern provinces such as Guangdong, Guangxi, and Yunnan. The traditional Chinese medicine gamboge (gamboge) has the functions of reducing swelling and attacking poison, stopping bleeding and killing insects. Garcinia OC (Oblongifolin C) is a natural small molecule isolated from Gamboge yunnanensis and Garcinia microphylla according to its chemical structure. phenolic) compounds. [0003] However, the research on lutein OC still needs to be improved. Contents of the invention [0004] The present invention aims at solving one of the above technical problems at least to a ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00
CPCA61K31/366A61P35/00
Inventor 许乃寒劳远至徐宏喜刘珍艳张雅鸥谢伟东
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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