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Porcine insulin super-molecular aggregate and preparation method thereof

A technology of supramolecular aggregates and insulin is applied in the field of biomedicine to achieve the effects of simple process, easily available raw materials and convenient operation.

Inactive Publication Date: 2013-07-10
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this work only studied bovine insulin supramolecular aggregates (B-SIA) and recombinant human insulin supramolecular aggregates (rH-SIA)

Method used

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  • Porcine insulin super-molecular aggregate and preparation method thereof
  • Porcine insulin super-molecular aggregate and preparation method thereof
  • Porcine insulin super-molecular aggregate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] According to the preparation method of the present invention, porcine insulin powder, phosphate buffer saline (PBS buffer) with a pH of 6.8, a 100ml flask, a stirring bar, and a constant temperature magnetic stirrer are prepared.

[0038] Weigh 50 mg of porcine insulin powder and put it into a flask equipped with a stirring bar, add 50 ml of PBS buffer solution with a pH of 6.8 to obtain a reactant solution, and put the gained reactant solution into a constant temperature magnetic stirrer whose temperature is adjusted to 37° C. The reaction was stirred at 150r / min, and under normal pressure, the stirring time was 15h, then the stirring reaction was stopped, and the suspension containing porcine insulin supramolecular aggregate P-SIA-1 was obtained.

Embodiment 2

[0040] According to the preparation method of the present invention, porcine insulin powder, PBS buffer solution with a pH of 7.2, a 50ml flask, a stirring bar, and a constant temperature magnetic stirrer are prepared.

[0041] Weigh 30 mg of porcine insulin powder and put it into a flask equipped with a stirrer, add 15 ml of PBS solution with a pH of 7.2, put the resulting reactant solution into a constant temperature magnetic stirrer with a temperature adjusted to 37°C, and stir at 100r / min Stir the reaction, under normal pressure, stop the reaction when the stirring time reaches 22 hours, and obtain the suspension of porcine insulin supramolecular aggregate P-SIA-2.

Embodiment 3

[0043] According to the preparation method of the present invention, porcine insulin solution, PBS buffer solution with a pH of 5.0, a 100ml flask, a stirring bar, and a constant temperature magnetic stirrer are prepared.

[0044] Weigh 100 mg of porcine insulin solution and put it into a flask equipped with a stirrer, add 50 ml of PBS buffer solution with a pH of 5.0, put the resulting reactant solution into a constant temperature magnetic stirrer whose temperature is adjusted to 37 ° C, and rotate at 200 r / min The reaction was stirred under normal pressure, and the reaction was stopped when the stirring time reached 100 h, and the suspension of porcine insulin supramolecular aggregate P-SIA-3 was obtained.

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Abstract

The invention relates to a porcine insulin super-molecular aggregate and a preparation method thereof. The invention belongs to the field of biomedicine. According to the invention, porcine insulin and a solution are prepared into a reactant solution; the reactant solution is subjected to a stirring reaction, such that the porcine insulin super-molecular aggregate (P-SIA) is prepared. According to the invention, porcine insulin monomers are subjected to protein senior structure change, and the porcine insulin monomers are aggregated through super-molecular interaction, such that the porcine insulin super-molecular aggregate is formed. The porcine insulin super-molecular aggregate has an expended and stretched morphology relative to the porcine insulin monomer. The method provided by the invention has the advantages of simple operation and easy-to-obtain raw materials. As a result of animal experiments, with single dose of the porcine insulin super-molecular aggregate, blood glucose concentration in rat can be effectively controlled for 129 days without fasting. Therefore, the obtained product has the potential to be used in long-effect in-vivo blood glucose concentration control, and a novel concept is brought to diabetic patient treatments. The aggregate and the method have wide application prospects in the field of biomedicine.

Description

technical field [0001] The invention relates to a technology for preparing insulin supramolecular aggregates, in particular to a method for preparing porcine insulin supramolecular aggregates (P-SIA) and the porcine insulin supramolecular aggregates, belonging to the field of biomedicine. Background technique [0002] Diabetes mellitus is a chronic systemic metabolic disease characterized by persistent high blood sugar. The reason is mainly due to the absolute lack of insulin secretion in the body or the relative lack of insulin caused by the body's increased demand for insulin, or insulin resistance. , thus leading to a syndrome of sugar, protein, and fat metabolism disorders mainly based on glucose metabolism disorders. As the course of the disease prolongs, it can lead to complications in tissues and organs such as eyes, nerves, blood vessels, and kidneys. Therefore, diabetes has become the third chronic disease that seriously threatens human health after tumors and card...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/28A61P5/48C07K14/62
Inventor 李建树罗珺
Owner SICHUAN UNIV