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Colorimetric detection probe of mercury ion and application method thereof

A technology for detection probes and colorimetric probes, applied in the measurement of color/spectral properties, analysis by chemical reaction of materials, and material analysis by observing the impact on chemical indicators, etc. It is easy to operate, the method is simple, and the selectivity is good.

Active Publication Date: 2015-02-04
GUANGDONG GAOPU QUALITY TECH SERVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, nucleic acid aptamers (aptamers) or DNA enzymes have been used as functional reagents to prepare probes with gold nanoparticles for the detection of Hg 2+ [Xue X J, Wang F, Liu X G, J. Am. Chem. Soc. 2008, 130, 3244–5; Liu C W, Hsieh Y T, Huang C C, Lin Z H, Chang H T, Chem. Comm. 2008, 2242–4; Wang H, Wang Y X, Jin J Y, Yang R H, Anal. Chem. 2008, 80, 9021–9028; Ye B C, Yin B C, Angew. Chem. Int. Ed. 2008, 47, 8386–9; Liu B, Biosensors and Bioelectronics , 2008, 24, 756-760; Yu C J, Cheng T L, Tseng W L, Biosensors and Bioelectronics , 2009, 25, 204-210.] and other heavy metal ions can achieve good sensitivity and selectivity, but due to the high cost of DNA and enzymes, they cannot be widely used

Method used

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  • Colorimetric detection probe of mercury ion and application method thereof
  • Colorimetric detection probe of mercury ion and application method thereof

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Experimental program
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Effect test

Embodiment 1

[0028] Detection of Hg using lipoic acid functionalized gold nanoparticles (TA-GNPs) as a colorimetric probe for detection 2+ When using a sample with a pH value in the range of 5 as a probe, dilute the colorimetric probe lipoic acid-functionalized gold nanoparticles by 4 times, then add the same amount of biological tissue or environmental sewage samples to be tested, and observe its Color changes when there is Hg 2+ When it exists, lipoic acid functionalized gold nanoparticles will change from the original red to purple or gray. At the same time, the ultraviolet spectrum test is carried out, and the surface plasmon characteristic absorption of gold nanoparticles is observed from the ultraviolet spectrum. When there is Hg in the solution 2+ When , the characteristic absorption peak of the solution is red-shifted, and when there is Hg in the solution 2+ When the concentration is very high, the gold nanoparticles are agglomerated, two absorption peaks will appear, and the c...

Embodiment 2

[0030] Detection of Hg using lipoic acid functionalized gold nanoparticles (TA-GNPs) as a colorimetric probe for detection 2+ When using a sample with a pH value in the range of 7 as a probe, dilute the colorimetric probe lipoic acid-functionalized gold nanoparticles by 3 times, then add the same amount of biological tissue or environmental sewage sample to be tested, and observe its Color changes when there is Hg 2+ When it exists, lipoic acid-functionalized gold nanoparticles will change from the original red to purple or gray. At the same time, the ultraviolet spectrum test is carried out to observe the surface plasmon characteristic absorption of gold nanoparticles from the ultraviolet spectrum. The characteristic absorption of gold nanoparticles is at 520nm There is a peak at , when Hg is present in the solution 2+ When , the characteristic absorption peak of the solution is red-shifted, and when there is Hg in the solution 2+ When the concentration is very high, the ...

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Abstract

The invention belongs to the technical field of detection, and concretely relates to a method of colorimetric detection of heavy-metal mercury ions in aqueous solution. The method utilizes lipoic acid functionalized gold nanoparticles as colorimetric probes. Two mercapto groups of the lipoic acid are utilized to combine and coordinate with the gold nanoparticles to form stable structure, and a pH value of the lipoic acid functionalized gold nanoparticles is adjusted so that surfaces of the lipoic acid functionalized gold nanoparticles contain a large number of free carboxyl groups. During a detection process, coordination of the carboxyl and the heavy-metal mercury ions is easy to occur, and by using character of plasma resonance on surfaces of the gold nanoparticles, existence of the heavy-metal mercury ions in the aqueous solution can be indicated through a color change of the solution from red to purple. The probe is high in sensitivity, good in selectivity and free of large-scale apparatuses, can realize in-situ rapid detection, with visual detection results which can be observed by the naked eyes, and is simple to operate and low in cost; and the reagents and operation process are free of toxic and side effects.

Description

technical field [0001] The invention belongs to the technical field of nanometer detection, and in particular relates to a mercury ion colorimetric detection probe and an application method thereof, and the application of the probe in aqueous solution to selectively identify heavy metal ions. Background technique [0002] Heavy metal ions (mercury Hg 2+ ; lead Pb 2+ ; Cadmium Cd 2+ ; Copper Cu 2+ ), especially mercury, cadmium, lead, copper, etc. have significant biological toxicity. Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. It is mainly caused by human factors such as mining, exhaust gas discharge, sewage irrigation and use of heavy metal products. Heavy metals that exist in various chemical states or chemical forms will persist, accumulate and migrate after entering the environment or ecosystem, causing harm. Among them, mercury is a common heavy metal in the environment with high toxicity. It exists in dif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/33
Inventor 柴芳苏东悦马亚杰杨馨夏清冬
Owner GUANGDONG GAOPU QUALITY TECH SERVICE CO LTD