Unlock instant, AI-driven research and patent intelligence for your innovation.

A rapid detection method of NMR foodborne pathogens based on paramagnetic nanometer Ni probe

A kind of food-borne pathogenic bacteria, paramagnetic nanotechnology, applied in the direction of measuring devices, analysis by nuclear magnetic resonance, instruments, etc.

Active Publication Date: 2016-04-13
苏州辅测技术服务有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature reporting this method at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] Examine food samples to determine whether they contain the harmful pathogen Listeria monocytogenes.

[0019] 1. Preparation of nano-Ni immunoprobes: Purchase nano-Ni materials from the market, such as Beijing Deke Daojin Technology Co., Ltd. or Shanghai Chaowei Nanomaterials Co., Ltd., 20nm, with a purity of 99.9%.

[0020] Silica-coated nano-Ni: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-Ni and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85°C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated three times to ensure that silicon is a...

example 2

[0027] Determination of food samples for the presence of harmful pathogenic bacteria Escherichia coli O157:H7.

[0028] 1. Nano-Ni immunoprobe preparation: buy nano-Ni materials from the market, such as Beijing Deke Daojin Technology Co., Ltd. or Shanghai Chaowei Nanomaterials Co., Ltd., 20nm, with a purity of 99.9%.

[0029] Silica-coated nano-Ni: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-Ni and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85°C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated three times to ensure that silicon is attac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses an NMR rapid detection method for food-borne pathogenic bacteria based on a paramagnetic nano-Ni probe, which belongs to the technical field of rapid detection of food-safe pathogenic bacteria. The present invention relies on the established NMR detection method that can be used for pathogenic bacteria in food samples, utilizes the influence of the paramagnetic properties of the paramagnetic nano-Ni probe on the relaxation time of the NMR attenuation signal, and detects whether the sample contains target bacteria. The different specific corresponding relationships are: paramagnetic nano-Ni probes show a linear relationship under certain conditions, that is, the content of nano-Ni is large, and the spin-lattice relaxation time and spin-spin relaxation time values ​​of the sample The smaller the value, the quantitative detection of target bacteria can be carried out within a certain range. The method can be used for rapid detection of harmful pathogenic bacteria in food samples, and thus can be used as a rapid screening of a large number of samples to be tested.

Description

technical field [0001] The invention relates to a rapid detection method for pathogenic bacteria, in particular to an NMR rapid detection method for food-borne pathogenic bacteria based on nano-Ni probes. Background technique [0002] Basic principle: Monoclonal antibodies or antigen molecules are combined with enzyme molecules through covalent bonds. This combination will not change the immunological characteristics and biological activities of monoclonal antibodies, antigens and enzymes. Specific monoclonal antibodies will only interact with specificity. antigen binding. Ni material has ferromagnetism, and paramagnetic properties will appear when the particle diameter is small enough to a certain extent. That is, there is no magnetism when there is no external magnetic field, but it shows certain magnetism when there is an external magnetic field, which can be used for magnetic separation. At the same time, the influence of paramagnetic substances on NMR signals is very ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N24/08G01N33/577
Inventor 张锦胜唐群赖卫华
Owner 苏州辅测技术服务有限公司