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Mycelium extract for helicobacter pylori-resistant biological medicine and preparation technology thereof
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A technology of Helicobacter pylori and extract, applied in the field of biopharmaceuticals
Active Publication Date: 2013-08-07
HUNAN XINHUI PHARMA
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[0008] At present, screening and discovering active ingredients from higher fungi and their cultures, and applying them to anti-tumor, anti-virus, diabetes, etc., has not only become the development direction of national key research new drugs, but also a hot spot in the development of biomedicine in various countries in the world today. There is no report that screens and discovers active ingredients from medicinal fungi and applies them to anti-Helicobacter pylori
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Embodiment 1
[0046] An anti-helicobacter pylori biomedical mycelium extract, the extract is prepared according to the following steps:
[0047](1) Take Hericium erinaceus culture, reflux extraction with ethanol with volume percentage concentration of 50% for 1h, then centrifuge at 4000r / min for 8min, remove the medicinal residues, and obtain the extract; wherein, during reflux extraction, Hericium erinaceus The weight ratio of culture to ethanol is 1:3;
[0048] (2) Rotary steam the extract at 50°C until there is no alcohol smell, add water to precipitate the precipitate, then centrifuge at 4000r / min for 8 minutes, and dry the precipitate at -60°C to obtain anti-Helicobacter pylori Biopharmaceutical mycelium extract.
Embodiment 2
[0050] An anti-helicobacter pylori biomedical mycelium extract, the extract is prepared according to the following steps:
[0052] (2) Rotary steam the extract at 50°C until there is no alcohol smell, add water to precipitate the precipitate, then centrifuge at 4000r / min for 8 minutes, and dry the precipitate at -40°C to obtain anti-H. pylori Biopharmaceutical mycelium extract.
Embodiment 3
[0054] An anti-helicobacter pylori biomedical mycelium extract, the extract is prepared according to the following steps:
[0055] (1) Take Hericium erinaceus culture, reflux extraction with 80% ethanol by volume percentage concentration for 2 hours, then centrifuge at 4000r / min for 8 minutes, remove the medicinal residues, and obtain the extract; wherein, during reflux extraction, Hericium erinaceus The weight ratio of culture to ethanol is 1:6;
[0056] (2) Rotary steam the extract at 80°C until there is no alcohol smell, add water to precipitate the precipitate, then centrifuge at 4000r / min for 8 minutes, and dry the precipitate at -60°C to obtain anti-H. pylori Biopharmaceutical mycelium extract.
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Abstract
The invention discloses mycelium extract for a helicobacter pylori-resistant biological medicine and a preparation technology thereof. Hericium erinaceus culture is subjected to reflux-extraction by ethanol having a volume concentration of 40 to 80%; extracting solution is subjected to rotary evaporation at a temperature of 30 to 80 DEG C; and the evaporation products are added with water and precipitates are separated out. The mycelium extract breaks the principle that the prior art mainly utilizes common antibiotics such as amoxicillin, gentamycin, clarithromycin and amoxicillin and an auxiliary acid-inhibition agent (such as a bismuth agent) to treat helicobacter pylori-caused infection, and has obvious treatment effects and small side-effect.
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