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Method for determining fat soluble green tea polyphenol content

A technology of fat-soluble tea polyphenols and tea polyphenols, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of inaccurate determination of fat-soluble tea polyphenols content, etc., achieve simple and feasible measurement methods, reduce costs, and be easy to popularize and apply Effect

Inactive Publication Date: 2013-08-07
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem that the content of fat-soluble tea polyphenols cannot be accurately measured in the prior art, the embodiment of the present invention provides a method for measuring the content of fat-soluble tea polyphenols

Method used

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  • Method for determining fat soluble green tea polyphenol content
  • Method for determining fat soluble green tea polyphenol content
  • Method for determining fat soluble green tea polyphenol content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] When tea polyphenol is used as raw material, and the acylating agent is stearic acid halide or stearic anhydride, the fat-soluble tea polyphenol is tea polyphenol stearate, and the content of tea polyphenol stearate is determined.

[0072] The reference substance is tea polyphenol stearate, and its ultraviolet wavelength scanning diagram of tea polyphenol stearate is shown in Figure 2.

[0073] According to the maximum absorption wavelength of the reference substance solution and the test solution is 275nm, then it is determined that the detection wavelength is 275nm and the error is ± 2nm, that is, the detection is effective within the 275nm error of ± 2nm.

[0074] Take 50 mg of dry tea polyphenol stearate, dissolve it with absolute ethanol and make it up to 100 mL, take 0, 2, 4, 6, 8 and 10 mL of the solution respectively in a 50 mL volumetric flask, and make it up to volume with absolute ethanol , to obtain the reference substance solution containing 0, 20, 40, 60, ...

Embodiment 2

[0078] When tea polyphenol is used as raw material, and the acylating agent is stearic acid halide or stearic anhydride, the fat-soluble tea polyphenol is tea polyphenol stearate, and the content of tea polyphenol stearate raw material is determined.

[0079] According to the maximum absorption wavelength of the reference substance solution and the test solution is 275nm, then it is determined that the detection wavelength is 275nm and the error is ± 2nm, that is, the detection is effective within the 275nm error of ± 2nm.

[0080]The reference substance is epigallocatechin gallate stearate, which is the reaction product of epigallocatechin gallate contained in tea polyphenols and stearic acid halide or stearic anhydride. The ultraviolet wavelength scanning picture of vegetarian gallate stearate is as follows image 3 shown.

[0081] Get dry epigallocatechin gallate stearate, dissolve with organic solvent, be mixed with every milliliter containing epigallocatechin gallate ste...

Embodiment 3

[0085] When tea polyphenols are used as raw materials and the acylating agent is palmitic acid halide or palmitic anhydride, the fat-soluble tea polyphenol is tea polyphenol palmitate, and the content of the preparation of tea polyphenol palmitate is determined, specifically for the determination Content of tea polyphenol palmitate gel.

[0086] The reference substance is epigallocatechin gallate palmitate, which is the reaction product of epigallocatechin gallate contained in tea polyphenols and palmitic acid halide or palmitic anhydride.

[0087] According to the maximum absorption wavelength of the reference substance solution and the test solution is 275nm, then it is determined that the detection wavelength is 275nm and the error is ± 2nm, that is, the detection is effective in the 275nm error within the wavelength range of ± 2nm.

[0088] Take the dried epigallocatechin gallate palmitate, dissolve it with an organic solvent, and prepare the preparations containing 0, 20,...

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Abstract

Belonging to the field of chemical quantitative detection, the invention discloses a method for determining a fat soluble green tea polyphenol content. The method comprises: preparing a reference substance and a test sample into a reference substance solution and a test sample solution respectively; drawing ultraviolet wavelength scan graphs of the reference substance solution and the test sample solution respectively to determine detection wavelength; drawing a standard curve of the reference substance solution and calculating a regression equation of the standard curve; determining absorbance of the test sample solution; and introducing the absorbance into the standard curve regression equation to calculate the fat soluble green tea polyphenol content in the test sample. By means of content determination on fat soluble green tea polyphenols and fat soluble green tea polyphenol-containing preparations, the method provided in the invention avoids indirect reflection of the fat soluble green tea polyphenol content by determining a phenolic hydroxyl group content, so that a color developing agent or an oxidant is unnecessary, the use of expensive standard substances and apparatuses can be avoided, and the cost is reduced. Also, the determination method is simple and accurate.

Description

technical field [0001] The invention relates to the field of chemical quantitative detection, in particular to a method for measuring the content of fat-soluble tea polyphenols. Background technique [0002] Green Tea Polyphenols (GTP) is a general term for more than 30 polyhydric phenolic compounds in tea, and catechins account for about 65% to 80% of the content of tea polyphenols, mainly including: (+) catechins Theophylline [(+) Catechin, C], (-) Epicatechin [(-) Epicatechin, EC], (-) Gallocatechin [(-) Gallocatechin, GC], (-) Epicatechin Gallate [(-) Epigallocatechin Gallate, ECG], (-) Epigallocatechin [(-) Epigallocatechin, EGC], (-) Epigallocatechin Gallate [(-) Epigallocatechin Gallate), EGCG ] Six substances are all flavan-3 alcohol derivatives. Among them, EGCG accounts for 50% to 60% of catechin compounds, and is a representative component of tea polyphenols in terms of structure, composition, and biological activity. [0003] Tea polyphenol is a kind of pure n...

Claims

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Application Information

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IPC IPC(8): G01N21/35G01N21/33
Inventor 张涛
Owner JIANGHAN UNIVERSITY
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