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Quinclorac degrading bacteria and application thereof

A technology of quinclorac and degrading bacteria, applied in the field of biological treatment of environmental pollutants, can solve the problems of slow degradation, affecting the yield and quality of tobacco leaves, and having no protective effect, and achieves the effect of preventing and controlling phytotoxicity

Active Publication Date: 2013-08-14
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Quinclorac is acidic and degrades slowly in acidic soil, which can easily cause serious damage to sensitive crops. Especially in rice and tobacco rotation areas, residual quinclorac in the soil is likely to cause harm to the growth of Solanaceae crop tobacco. , seriously affecting the yield and quality of tobacco leaves, there is currently no effective method or agent for protecting tobacco from the phytotoxicity of quinclorac residues
However, there is no protection against tobacco injury from quinclorac
And there is no research report on the degradation of quinclorac by Alcaligenes at home and abroad

Method used

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  • Quinclorac degrading bacteria and application thereof
  • Quinclorac degrading bacteria and application thereof
  • Quinclorac degrading bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Screening and activity detection of degrading bacteria J3

[0025] In this experiment, a degrading bacterium with good degrading ability to quinclorac was isolated from three soil samples collected for long-term application of quinclorac by means of enrichment culture and smearing, and it was named for J3. Electron micrographs of strain J3 as figure 1 shown.

[0026] Three soil samples were collected from the paddy fields and tobacco fields near Hunan Agricultural University and the outfall of a pesticide factory in Hunan. ); Tobacco field soil near Hunan Agricultural University (J).

[0027] Enrichment of quinclorac acidolytic bacteria: 5g of each soil sample was added to 50mL inorganic salt medium containing 100mg / L quinclorac, and cultured with shaking at 30°C and 180r / min for one week. Afterwards, the inoculation amount of 4% (volume fraction) was inserted into the freshly sterilized inorganic salt medium, transferred once a week, the concentration of ...

example 2

[0032] Identification of Example 2 bacterial strain J3

[0033] Morphological and physiological and biochemical characteristics of J3: The colony and single bacterial morphology of strain J3 were observed by microscope and scanning electron microscope. The physiological and biochemical identification of J3 uses a kit (single-box biochemical identification tube, produced by Guangdong Huankai Microbial Technology Co., Ltd.).

[0034] Determination of the 16S rDNA sequence of J3: the DNA of the degrading bacteria was extracted using a kit (DNA extraction kit, produced by Beijing Quanshijin Biotechnology Co., Ltd.), and the amplification primers used were synthesized by Huada Gene Company:

[0035] The upstream primer is 5'-AGAGTTTGATCMTGGCTCAG-3';

[0036] The downstream primer is 5'-TACGGCTACCTTGTTACGACTT-3'.

[0037] The amplification reaction system is: 10×Buffer (Mg 2+ ) 4 μL, dNTPs 3 μL, each primer 1 μL, cell DNA 2 μL, Taq DNA polymerase 1 μL, add deionized water to 50 μ...

example 3

[0042] Research on the degradation characteristics of example 3 degrading bacteria J3

[0043] The influence of culture time on the growth and degradation rate of degrading bacteria J3: Prepare 600ml inorganic salt medium and divide it into four 300ml Erlenmeyer flasks (150ml each), set one Erlenmeyer flask as CK, and the other three as three Repeat, adjust the pH to 7.0-7.2, and sterilize for later use. Add quinclorac to the sterilized Erlenmeyer flask to make the concentration 50mg / L, and insert the activated degrader into the other three Erlenmeyer flasks except CK with 4% inoculum (OD600=0.42). Bacteria J3 was then placed in a constant temperature shaker at 30°C and 150r·min-1 for shaking culture. Samples were taken every 24 hours to determine the OD600 value and degradation rate changes.

[0044] The effect of the initial concentration of quinclorac on the growth and degradation rate of degrading bacteria J3: prepare 2700ml inorganic salt culture medium and divide them ...

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Abstract

The invention discloses a quinclorac degrading bacteria and application thereof. The quinclorac degrading bacteria J3 belongs to Alcaligenes sp. and is collected in CGMCC (China General Microbiological Culture Collection Center); the collection date is March 28, 2013, the collection number is CGMCC No.7384, and the GenBank accession number of a bacterial strain 16S rDNA is KC693648. The quinclorac degrading bacteria is a Gram negative bacterium, and a bacterial colony has a smooth surface and a raised center, is in a faint yellow color and a circular shape and is regular on the edge and non-transparent. The quinclorac degrading bacteria disclosed by the invention has a quinclorac degradation function and can prevent the tobaccos from being injured by quinclorac.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and specifically relates to a quinclorac-degrading bacterium and its application in degrading quinclorac and preventing tobacco from being injured by quinclorac. Background technique [0002] Quinclorac (quinclorac), chemical name: 3,7-dichloro-8-quinoline carboxylic acid, is a new pre-emergence and post-emergence paddy field herbicide developed by BASF in Germany, because of its low dosage and long-lasting effect It is widely used in agricultural production because of its advantages of long lifespan and special effects on barnyardgrass, and is mainly used for preventing barnyardgrass in paddy fields. Its structural formula is as follows: [0003] [0004] Quinclorac is acidic and degrades slowly in acidic soil, which can easily cause serious damage to sensitive crops. Especially in rice and tobacco rotation areas, residual quinclorac in the soil is li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12R1/05C12N1/20A62D3/02
Inventor 柏连阳罗坤董俊宇周小毛曾爱平刘祥英胡利锋刘开林
Owner HUNAN AGRICULTURAL UNIV
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