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An endophytic Bacillus megaterium and its application in repairing quinclorac phytotoxicity

A technology of Bacillus megaterium and quinclorac, applied in the field of pesticide-contaminated microbial remediation, can solve problems such as slow degradation, growth hazards, and hazards of sensitive crops after cropping, and achieve the effects of reducing production losses and improving yield and quality

Active Publication Date: 2016-08-24
HUNAN ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Quinclorac is weakly acidic and degrades slowly in acidic soils, which can easily cause serious harm to sensitive crops after cropping. Especially in rice and tobacco rotation areas, the residual quinclorac in the soil is at a very low concentration (0.001mg / kg) can cause harm to the growth of crops such as Solanaceae, Cucurbitaceae, and Umbelliferae, and seriously affect the yield and quality of crops.
The degrading bacteria isolated above are all enriched and isolated from the soil, but there is no report on the endogenous degrading bacteria of quinclorac at present in China

Method used

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  • An endophytic Bacillus megaterium and its application in repairing quinclorac phytotoxicity
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  • An endophytic Bacillus megaterium and its application in repairing quinclorac phytotoxicity

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Embodiment 1

[0027] The isolation and identification of embodiment 1 bacterial strain

[0028] The present invention adopts the plate marking method to obtain an endogenous degrading bacterium Q3 that has a better degradation effect to quinclorac from the root system of tobacco damaged by quinclorac, and the Q3 colony morphology and scanning electron microscope photos are as follows: figure 1 , figure 2 shown.

[0029] 1. Culture medium:

[0030] Inorganic salt culture medium: 1 g of ammonium sulfate, 2 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate, 0.1 g of sodium chloride, 0.5 g of calcium carbonate, 1000 ml of deionized water, pH 7.0, autoclaved at 121°C for 30 min.

[0031] LB medium: beef extract 10g, peptone 5g, sodium chloride 10g (solid medium plus agar 15g), 1000mL deionized water, pH 7.0, autoclaved at 121°C for 30min.

[0032] 2. Isolation and purification of endogenous degrading bacteria

[0033] Take the root system of tobacco injured by quinclorac, rins...

Embodiment 2

[0044] Research on the degradation characteristics of embodiment 2 degrading bacteria Q3

[0045] The influence of medium pH on the growth and degradation rate of degrading bacteria Q3: prepare inorganic salt medium (adding a small amount of sucrose and yeast powder as additional carbon source) and divide them into Erlenmeyer flasks, and fill each bottle with 50ml of medium, adjust the medium The pH value is respectively 5, 6, 7, 8, 9 (each processing 3 repetitions), adds quinclorac after the sterilization treatment, makes the concentration of quinclorac in each Erlenmeyer flask be 20mg / L, Then insert the activated degrading bacteria Q3 bacterium solution with an inoculation amount of 5% (volume fraction), put it into 30 ℃, 180r min -1 Shaking culture in a constant temperature shaker. After 7 days, samples were taken to measure the OD 600 value and degradation rate.

[0046] The influence of culture temperature on the growth and degradation rate of degrading bacteria Q3: th...

Embodiment 3

[0051] The control effect of embodiment 3 bacterial strain Q3 to tobacco injury

[0052] Take K326 as the test tobacco. The preserved Q3 strain was transferred to sterilized liquid LB medium, and cultured in the dark at 30°C and 180r / min constant temperature shaker overnight.

[0053] Get the soil with the recommended dose of quinclorac in the previous paddy field, air-dry and pulverize it, and put it into pots with a diameter of 18 cm and a height of 20 cm, and each pot is filled with 3 kg of soil. 7 days before the tobacco seedlings were transplanted, use the Q3 fermentation liquid (Q3 was cultivated and fermented through the night, OD 600 =0.63, after diluting 10ml fermented liquid of every plant with 200ml water, evenly apply it to pots and bowls. ) for soil treatment. Set up the blank control group (cultivation in soil without quinclorac residues, no phytotoxicity), the phytotoxicity control group (soil with quinclorac residues, no degrading bacteria treatment) and the...

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Abstract

The invention relates to a strain of bacillus megaterium and its application in repairing tobacco quinclorac phytotoxicity. The Bacillus megaterium Q3 is deposited in the General Microorganism Center (CGMCC) of the China Committee for Culture Collection of Microorganisms, and the preservation number is: CGMCC No.8576. The Bacillus megaterium Q3 of the present invention can effectively degrade low quinclorac concentrations, and can be applied to water bodies and soils polluted by quinclorac, and can be used to alleviate quinclorac phytotoxicity in tobacco production.

Description

technical field [0001] The invention belongs to the field of remediation of pesticide-contaminated microorganisms, and in particular relates to a strain of endophytic Bacillus megaterium and its application in remediation of quinclorac phytotoxicity. Background technique [0002] Quinclorac (quinclorac), chemical name: 3,7-dichloro-8-quinoline carboxylic acid, is a new type of paddy field herbicide before and after emergence developed by BASF, Germany, because of its low dosage and long-lasting effect It is widely used in agricultural production because of its advantages of long lifespan and special effects on barnyardgrass, and is mainly used for preventing barnyardgrass in paddy fields. Its structural formula is as follows: [0003] [0004] Quinclorac is weakly acidic and degrades slowly in acidic soils, which can easily cause serious harm to sensitive crops after cropping. Especially in rice and tobacco rotation areas, the residual quinclorac in the soil is at a very...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/11
Inventor 柏连阳刘敏罗坤曾爱平周小毛罗峰刘祥英胡利锋刘开林
Owner HUNAN ACADEMY OF AGRI SCI
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