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Microbial organic fertilizer specially used for strawberries, as well as preparation method and application thereof

A technology of microbial organic fertilizer and organic fertilizer, which is applied in the direction of organic fertilizer, etc., to achieve the effect of enhancing water retention, good control effect, and enhancing disease resistance

Inactive Publication Date: 2013-08-21
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few reports on the development and application of microbial organic fertilizers for strawberries, especially microbial organic fertilizers with high-efficiency and stable nutrition and disease prevention and growth-promoting effects.

Method used

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  • Microbial organic fertilizer specially used for strawberries, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example is used to illustrate the fermentation of the SB177 strain.

[0031] A single colony of the SB177 strain activated on LB solid medium was transferred to a 20mL Erlenmeyer flask containing 5mL of PDA liquid medium, and cultured with shaking at 30°C and 200rpm for 16h. Inoculate the culture solution obtained in the previous step into a 1000mL Erlenmeyer flask containing 400mL of PDA medium, shake it at 200rpm and 30°C for 16 hours, then inoculate it in a mechanically stirred fermenter containing 20L of fermentation medium, and ferment The conditions are set as follows: dissolved oxygen 100%, stirring speed 350rpm, fermentation temperature 30°C, fermentation time 48h, pH 7.0-7.2. Wherein the formula of the fermentation medium is: soybean flour 5g / L, corn flour 5g / L, soluble starch 5g / L, sucrose 1.25g / L, yeast powder 1.25g / L, calcium carbonate 1g / L, MnSO 4 0.05g / L, pH 7.2-7.4. After the fermentation reaches the end point, take a sample to detect the concentr...

Embodiment 2

[0033] This example is used to illustrate the fermentation of the TX-4 strain.

[0034]A single colony of the TX-4 strain activated on NA solid medium was transferred to a 20mL Erlenmeyer flask containing 5mL of PDA liquid medium, and cultured with shaking at 30°C and 200rpm for 16h. Inoculate the culture solution obtained in the previous step into a 1000mL Erlenmeyer flask containing 400mL of PDA medium, shake it at 200rpm and 30°C for 16 hours, then inoculate it in a mechanically stirred fermenter containing 20L of PDA medium, and ferment The conditions are set as follows: dissolved oxygen 100%, stirring speed 350rpm, fermentation temperature 30°C, fermentation time 48h, pH 7.0-7.2. After the fermentation reaches the end point, after the fermentation reaches the end point, take a sample to detect the concentration of live spores to ensure that the concentration of live spores is ≥ 1.0×10 10 cfu / mL, and finally collect the bacterial solution for later use.

Embodiment 3

[0036] This example is used to illustrate the fermentation of NF0919 strain.

[0037] Activate the preserved NF0919 strain on Gaoshi No. 1 solid medium, culture at 30°C for 10 days, and pick 1 cm 2 The large and small bacterial blocks were inoculated in a 250mL Erlenmeyer flask containing 50mL of the primary seed culture solution, placed on a shaker at 170rpm at 30°C for 44-48h, and the formula of the above-mentioned primary seed culture solution was: soluble starch 30 g / L , glucose 0 g / L, yeast powder 10 g / L, peptone 10 g / L, NaCl 5 g / L, K 2 HPO 4 2 g / L, pH 7.2-7.4.

[0038] Put the primary seed culture solution obtained in the above steps into a 250mL Erlenmeyer flask containing 50mL of the secondary seed culture solution at a volume ratio of 5%, place it on a shaker at 170rpm at 30°C for 48-52h, and culture it for 48-52h. The formula of the seed culture solution is: potato starch 85g / L, cottonseed egg 22g / L, CaCO 3 1g / L, K 2 HPO 4 0.5g / L, MgSO 4 0.5 g / L, amylase 0.1 ...

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Abstract

The invention relates to a microbial organic fertilizer specially used for strawberries; the microbial organic fertilizer is prepared from a living bacteria agent of bacillus amyloliquefaciens SB177 bacterial strains, a living bacteria agent of paenibacillusspp. TX-4 bacterial strains, active fermented liquid of streptomycescorchorusii NF0919 bacterial strains, humic acid and argil; and the weight ratio of all the components is 8:8:(5-10):(70-80):(3-5). The invention also provides a preparation method and an application of the microbial organic fertilizer. The microbial organic fertilizer has nutrition, has the functions of disease prevention and growth promotion, and solves the problem of successive cropping obstacle caused in the cultivation of the strawberries.

Description

technical field [0001] The present invention relates to microbial fertilizer and its preparation method and application, more specifically to a kind of special microbial organic fertilizer with nutrition and disease prevention and growth promotion function and its preparation method, and the application of the microbial organic fertilizer in strawberry cultivation . Background technique [0002] Strawberry is a commodity crop with high economic value, which is planted in all provinces, autonomous regions and municipalities directly under the central government in my country. According to incomplete statistics, the total area of ​​strawberry planting in my country reached 113989hm in 2010 2 , the total output is 2 million tons, and the total output value has exceeded 20 billion, thus becoming the world's largest strawberry production and consumption country. The strawberry industry has become a pillar industry in many areas, and strawberry professional villages, strawberry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08
Inventor 陈宏州杨敬辉吴祥吉沐祥庄义庆
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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