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Extraction method of dendrobine

A purification method and dendrobine technology, applied in the field of natural medicinal chemistry, can solve the problems of dendrobine pollution, low product yield, complicated process operation, etc.

Inactive Publication Date: 2013-08-28
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing process for preparing dendrobine has serious pollution, low product yield, complicated process operation, and is not suitable for industrialization

Method used

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Embodiment 1

[0016] Example 2:

Embodiment 2

[0018] Example 3:

Embodiment 3

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Abstract

The invention discloses an extraction method of dendrobine, which is simple and convenient to operate and small in pollution. The method comprises the technical steps of: 1) crushing a dendrobium stem raw material to 20-80 meshes, infiltrating by alkaline water, placing in a CO2 supercritical extraction tank to extract, wherein the CO2 flow is 1-5ml / min / g raw material, selecting 90% acetone as an entrainer, wherein the flow is 0.1-0.5ml / min / g raw material, the extraction temperature is 30-60 DEG C, the pressure is 20-30MPa and the extraction time is 1-4 hours, dissolving the extract by chloroform, filtrating and recovering chloroform to obtain a crude extract; and 2) separating and purifying by high speed counter current chromatography by using chloroform, methanol and water as a solvent system for the crude extract, and collecting the fraction according to the chromatogram and decompressing and drying the fraction to obtain dendrobine. With the adoption of the method for extracting dendrobine, the purity of the product is high, and the method is easy to realize industrial amplification.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a method for purifying dendrobine. Background technique [0002] Dendrobine, a tetrahydropyrrole alkaloid, is a hydrophobic weak base with molecular formula C 16 h 25 NO 2 , molecular weight 263.27. [0003] Dendrobine has analgesic, antipyretic, anti-tumor effects, can lower heart rate, blood pressure, slow down breathing, and can relieve barbiturate poisoning, so it has high research and development value. [0004] Most of the existing techniques are also extracted from Dendrobium nobile, such as "Automatic Potentiometric Titration Determination of the Content of Total Alkaloids in Dendrobium Nobile Medicinal Materials" published by Hua Moli, etc., the method disclosed in this document is high alcohol reflux extraction, concentrated to The extract is dissolved in hydrochloric acid, then applied to strong acid cationic resin, eluted with 5% ammonia wate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/08
CPCY02P20/54
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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