Method for quickly culturing verticillium dahliae

A rapid technology for Verticillium dahliae, applied in the field of rapid cultivation of Verticillium dahliae, can solve the problems of long cultivation time, unsatisfactory mycelium or conidia, easy contamination by miscellaneous bacteria, etc., and achieve practical Strong performance, strong promotion and application value, and rapid training effect

Inactive Publication Date: 2013-08-28
INNER MONGOLIA AGRICULTURAL UNIVERSITY
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for quickly cultivating Verticillium dahliae, aiming to solve the problem that Verticillium dahliae grows very slowly in PDA medium and cannot obtain a large amount of mycelium or conidia in a short time Requirements; at the same time, due to the long cultivation time, it is easy to be contaminated by bacteria during the cultivation process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quickly culturing verticillium dahliae
  • Method for quickly culturing verticillium dahliae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] 1) Select a strain of Verticillium dahliae bacterial strain, after cultivating on the PDA medium, directly get the bacterial block from the medium;

[0030] 2) Wash the bacterial block with 1ml sterile water to obtain the conidia liquid;

[0031] 3) absorb the spore droplet onto the PDA medium covered with sterilized filter paper, and incubate at 25°C for 7 days;

[0032] Scrape mycelium into a 1.5ml centrifuge tube for DNA extraction. Or wash the PDA medium with sterile water to prepare conidia liquid.

[0033] The invention can obtain a large amount of mycelia and conidia of Verticillium dahliae in a short time, so as to be used for DNA extraction or prepare conidia liquid for inoculation. figure 2 The upper figure in the middle is the colony form after 7 days of cultivation by the present invention, and the lower figure is the colony form after 7 days of cultivation by the conventional method.

[0034] In the method for quickly cultivating Verticillium dahliae pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for quickly culturing verticillium dahliae. The method comprises steps of: firstly, culturing the verticillium dahliae on a potato dextrose agar (PDA) medium, and directly beating the medium to form verticillium dahliae blocks; then utilizing 1ml of sterile water to wash the verticillium dahliae blocks, and obtaining a conidium liquid; finally, sucking the conidium liquid, dipping the conidium liquid onto the PDA medium laid with sterilization filter papers, and culturing at 25 DEG C for 7 days; and scraping to obtain hyphae in a centrifugal pipe of 1.5ml, carrying out liquid nitrogen flash freezing, storing at -80 DEG C for extracting deoxyribose nucleic acid (DNA), and moreover, utilizing the sterile water to wash the PDA medium so as to prepare the conidium liquid. By utilizing the method, a purpose of simply, easily and quickly culturing the verticillium dahliae is realized, and the hyphae and conidia for DNA extraction and inoculation experiments can be greatly cultured within a short time, so that the method has strong practicability and strong popularization and application values.

Description

technical field [0001] The invention belongs to the technical field of cultivating Verticillium dahliae, in particular to a method for quickly cultivating Verticillium dahliae. Background technique [0002] At present, PDA medium is generally used to cultivate Verticillium dahliae. Because Verticillium dahliae grows very slowly even under sufficient nutrient conditions, it generally takes 15 days to cover the whole dish for scraping mycelia or Bacterial blocks cannot meet the requirement of obtaining a large amount of mycelium or conidia in a short period of time; at the same time, due to the long cultivation time, it is easy to contaminate miscellaneous bacteria during the cultivation process. Contents of the invention [0003] The invention provides a method for quickly cultivating Verticillium dahliae, aiming to solve the problem that Verticillium dahliae grows very slowly in PDA medium and cannot obtain a large amount of mycelia or conidia in a short time Requirements...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 张贵赵君周洪友景岚任杰曹雄田素萍张建张园园
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap