Ibor-Ins gene mutant from ipomoea batatas and uses thereof
A gene and sweet potato technology, applied to the IbOr-Ins gene mutant derived from sweet potato and its application field, can solve the problems of crop productivity loss, inability to cultivate, etc., and achieve the effect of improving functionality and strong tolerance to salt stress
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0047] In addition, the present invention provides a method for preparing transformed plants with increased carotenoid content, said preparation method comprising the steps of transforming the recombinant vector of the present invention into plant cells and overexpressing IbOr-Ins gene mutants.
[0048] In addition, the present invention provides a transformed plant and its seeds prepared by the above method with increased carotenoid content. The above-mentioned plants may be dicotyledonous plants, but are not limited thereto.
[0049]The above-mentioned dicotyledonous plants can be Prunaceae (Diapensiaceae), Alder family (Clethraceae), Wintergreens (Pyrolaceae), Rhododendrons (Ericaceae), Myrsinaceae (Myrsinaceae), Primulaceae ( Primulaceae), Plumbaginaceae, Ebenaceae, Styracaceae, Styracaceae, Symplocaceae, Oleaceae (Oleaceae), Loganiaceae, Dragon Gentianaceae, Menyanthaceae, Apocynaceae, Asclepiadaceae, Rubiaceae, Polemoniaceae, Convolvulaceae ), Boraginaceae, Verbenaceae...
Embodiment 1
[0058] Embodiment 1: Cloning and base sequence analysis of IbOr-Ins gene
[0059] Prepare PCR primers for cloning Orange gene from sweet potato, the primer sequences are as follows: forward primer (5'-atggtatattcaggtagaatcttgtcgctc-3'; SEQ ID No.3) and reverse primer (5'-ttaatcaaatgggtcaattcgtgggtcatg-3'; SEQ ID No .4). Using the thus obtained IbOr as a prototype, overlapping PCR was performed to artificially mutate the specific base sequence of IbOr. In order to insert the base sequence deduced as -KSQNPNL- from the 133th amino acid in the amino acid sequence of the IbOrange gene, PCR primers were prepared, and the primer sequences used at this time were as follows: Forward primer (5'-gaaaagcaagaaaataaacttaa atcccagaaccctaac-3 '; SEQ ID No.5) and a reverse primer (5'-aagatttgcggatgtcaggtt agggttctgggatttaag-3'; SEQ ID No.6). As a result, a PCR product of about 921bp was obtained, cloned into the pGEM-T-vector, and sequenced to confirm the mutation inserted with -KSQNPNL- fr...
Embodiment 2
[0061] Example 2: Phenotype Analysis of IbOrange and IbOr-Ins Transformed Sweet Potato Cultured Cells
[0062] Will image 3 The plant expression vector was transformed by Agrobacterium-mediated transformation into beige sweet potato Kurime ( ) in cultured cells. As a result, Kurimi, which is a non-transformant, showed no change in beige color, while IbOrange and IbOr-Ins transformed cultured cells showed dark yellow, and IbOr-Ins showed a darker color with the naked eye ( Figure 4 ).
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com