Ibor-Ins gene mutant from ipomoea batatas and uses thereof

A gene and sweet potato technology, applied to the IbOr-Ins gene mutant derived from sweet potato and its application field, can solve the problems of crop productivity loss, inability to cultivate, etc., and achieve the effect of improving functionality and strong tolerance to salt stress

Inactive Publication Date: 2013-08-28
KOREA RES INST OF BIOSCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In many areas of the world, due to the high salinity of the soil, the productivity loss of crops has reached more than 70%, or it is becoming barren land that cannot be cultivated.

Method used

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  • Ibor-Ins gene mutant from ipomoea batatas and uses thereof
  • Ibor-Ins gene mutant from ipomoea batatas and uses thereof
  • Ibor-Ins gene mutant from ipomoea batatas and uses thereof

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preparation example Construction

[0047] In addition, the present invention provides a method for preparing transformed plants with increased carotenoid content, said preparation method comprising the steps of transforming the recombinant vector of the present invention into plant cells and overexpressing IbOr-Ins gene mutants.

[0048] In addition, the present invention provides a transformed plant and its seeds prepared by the above method with increased carotenoid content. The above-mentioned plants may be dicotyledonous plants, but are not limited thereto.

[0049]The above-mentioned dicotyledonous plants can be Prunaceae (Diapensiaceae), Alder family (Clethraceae), Wintergreens (Pyrolaceae), Rhododendrons (Ericaceae), Myrsinaceae (Myrsinaceae), Primulaceae ( Primulaceae), Plumbaginaceae, Ebenaceae, Styracaceae, Styracaceae, Symplocaceae, Oleaceae (Oleaceae), Loganiaceae, Dragon Gentianaceae, Menyanthaceae, Apocynaceae, Asclepiadaceae, Rubiaceae, Polemoniaceae, Convolvulaceae ), Boraginaceae, Verbenaceae...

Embodiment 1

[0058] Embodiment 1: Cloning and base sequence analysis of IbOr-Ins gene

[0059] Prepare PCR primers for cloning Orange gene from sweet potato, the primer sequences are as follows: forward primer (5'-atggtatattcaggtagaatcttgtcgctc-3'; SEQ ID No.3) and reverse primer (5'-ttaatcaaatgggtcaattcgtgggtcatg-3'; SEQ ID No .4). Using the thus obtained IbOr as a prototype, overlapping PCR was performed to artificially mutate the specific base sequence of IbOr. In order to insert the base sequence deduced as -KSQNPNL- from the 133th amino acid in the amino acid sequence of the IbOrange gene, PCR primers were prepared, and the primer sequences used at this time were as follows: Forward primer (5'-gaaaagcaagaaaataaacttaa atcccagaaccctaac-3 '; SEQ ID No.5) and a reverse primer (5'-aagatttgcggatgtcaggtt agggttctgggatttaag-3'; SEQ ID No.6). As a result, a PCR product of about 921bp was obtained, cloned into the pGEM-T-vector, and sequenced to confirm the mutation inserted with -KSQNPNL- fr...

Embodiment 2

[0061] Example 2: Phenotype Analysis of IbOrange and IbOr-Ins Transformed Sweet Potato Cultured Cells

[0062] Will image 3 The plant expression vector was transformed by Agrobacterium-mediated transformation into beige sweet potato Kurime ( ) in cultured cells. As a result, Kurimi, which is a non-transformant, showed no change in beige color, while IbOrange and IbOr-Ins transformed cultured cells showed dark yellow, and IbOr-Ins showed a darker color with the naked eye ( Figure 4 ).

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Abstract

An IbOr-Ins mutant gene having specific nucleotide sequences inserted into IbOrange gene is provided to enhance carotenoid content and to develop a transgenic plant with salt stress tolerance. An IbOr-Ins mutant gene is prepared by inserting a specific nucleotide sequence into Ipomoea batatas-derived IbOrange gene and has a base of sequence number 2. A method for producing a transgenic plant with enhanced carotenoid content comprises a transforming a recombinant vector containing IbOr-Ins mutant gene into plant cells and overexpressing IbOr-Ins mutant gene. A composition for enhancing carotenoid content of a plant contains the IbOr-Ins mutant gene.

Description

technical field [0001] The present invention relates to the IbOr-Ins gene mutant derived from sweet potato and its use, and relates in more detail to the IbOr-Ins gene mutant artificially inserted into the specific base sequence in the IbOrange gene derived from sweet potato (Ipomoea batatas); The recombinant vector of the gene mutant; the host cell for transforming the recombinant vector; the method for preparing the transformed plant by transforming the recombinant vector into plant cells, thereby increasing the carotenoid content or salt stress tolerance; The carotenoid content or salt stress tolerance prepared by the above-mentioned method are increased transformed plants and seeds thereof; the preparation method of the transformed plants obtained by using the salt stress tolerance of the IbOrange gene derived from sweet potato is increased; the salt prepared by the method Transformed plants having increased stress tolerance and seeds thereof. Background technique [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00A01H5/10C12N15/29C12N15/82
CPCC07K14/415C12N15/825C12N15/8273C12N15/8243
Inventor 郭尚洙李幸顺安荣玉郑在哲金善荷
Owner KOREA RES INST OF BIOSCI & BIOTECH
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