Method for preparing traditional Chinese medicine microbial fertilizer of spinach
A microbial fertilizer and microbial technology, applied in the preparation of organic fertilizers, organic fertilizers, fertilizer mixtures, etc., can solve the problems of unstable nutrient content of organic fertilizers, unreasonable ratios of nutrient elements, and difficulty in ensuring product safety, etc. Medicinal properties, good absorption, and product safety effects
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Embodiment 1
[0025] A preparation method of spinach traditional Chinese medicine microbial fertilizer, comprising the following steps:
[0026] (1) Fermentation and cultivation of microorganisms:
[0027] ① Inoculate Bacillus amyloliquefaciens, Bacillus thuringiensis and Bacillus jelly-like on the slope of PDA medium, the initial pH of the medium is controlled at 6; the culture temperature is controlled at 20°C; after 20 hours of culture, the bacterial suspension is made , then inoculate the prepared bacterium suspension in the PDA liquid medium according to the inoculum size of 2% in proportion, and cultivate with shaking (shaking table rotation number is 160 rpm), and the temperature is controlled at 30° C., and cultivates for 48 hours;
[0028] ②Put Frankia, Ichthyospora, and Geodermophilus on the modified Camp-BAP medium, the initial pH of the medium is controlled at about 7, the temperature is 20°C, culture for 16 days, and the temperature is raised to 30°C to continue the culture Af...
Embodiment 2
[0038] A preparation method of spinach traditional Chinese medicine microbial fertilizer, comprising the following steps:
[0039] (1) Fermentation and cultivation of microorganisms:
[0040] ① Inoculate Bacillus amyloliquefaciens, Bacillus thuringiensis and Bacillus jelly-like on the slope of PDA medium, the initial pH of the medium is controlled at 7; the culture temperature is controlled at 30°C; after 16 hours of culture, the bacterial suspension is made , then inoculate the prepared bacterium suspension in the PDA liquid medium according to the inoculum size of 2% in proportion, and cultivate with shaking (shaking table rotation number is 160 rpm), and the temperature is controlled at 28° C., and cultivates for 56 hours;
[0041]②Put Frankia, Ichthyospora, and Geodermophilus on the modified Camp-BAP medium, the initial pH of the medium is controlled at about 7, the temperature is 22°C, culture for 14 days, and the temperature is raised to 29°C to continue the culture Aft...
Embodiment 3
[0051] 1, a kind of preparation method of spinach traditional Chinese medicine microbial fertilizer is characterized in that, comprises the following steps:
[0052] (1) Fermentation and cultivation of microorganisms:
[0053] ① Inoculate Bacillus amyloliquefaciens, Bacillus thuringiensis and Bacillus jelly-like on the slant of PDA medium, the initial pH of the medium is controlled at 8; the culture temperature is controlled at 40°C; after 12 hours of culture, the bacterial suspension is made , then inoculate the prepared bacterium suspension in the PDA liquid medium according to the inoculum size of 2% in proportion, and cultivate with shaking (shaking table rotation number is 160 rpm), and the temperature is controlled at 30° C., and cultivates for 48 hours;
[0054] ②Put Frankia, Ichthyospora, and Geodermophilus on the modified Camp-BAP medium. The initial pH of the medium is controlled at about 7, and the temperature is 25°C. Cultivate for 12 days, and then raise the tempe...
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