Cell scattered light multi-position detecting method as well as cell analyzer
A detection method and scattered light technology, which is applied in the direction of individual particle analysis, particle and sedimentation analysis, and measuring devices, can solve the problems of difficulty in further improving the accuracy and stability of the instrument, and increase the cost, so as to reduce the magnification and reduce the cost. Noise function requirements, reliability improvement, the effect of accuracy and stability improvement
Inactive Publication Date: 2013-09-04
URIT MEDICAL ELECTRONICS CO LTD
View PDF6 Cites 6 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
In order to detect the 90-degree scattered light information of cells, a high-gain photomultiplier tube has to be used, which cannot avoid increased noise, which increases the cost of the flow cytometer, and it is difficult to further improve the accuracy and stability of the entire instrument
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0020] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0021] Example of multi-position detection method for cell scattered light
[0022] Such as figure 1 As shown, the laser travels along the X-axis direction, the centerline of the sheath flow tube is set along the Y-axis direction, the cross-section of the sheath flow tube is rectangular, the front and rear tube walls are parallel to the XY plane, and the left and right tube walls are perpendicular to the laser traveling direction. When a single cell arranged in a row passes through the laser, the scattered light of the cells irradiated by the laser in the 90° direction is on the plane P that is 90° to the laser direction, that is figure 1 The YZ plane.
[0023] On the plane P, the scattered light information of the cells detected at the points with the same distance from the centerline of the sheath flow tube are identical. In this...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a cell scattered light multi-position detecting method as well as a cell analyzer. The method comprises the following steps of: taking 2-4 detecting points on a cell scattered light plane forming 90 degrees with a laser incidence direction, and detecting 90-degree cell scattered light, wherein the distances from the detecting points to a central line of a sheath flow pipe are equal, and are symmetrical about the central line of the sheath flow pipe. The cell analyzer comprises a laser radiation mechanism, a cell sheath flow mechanism, a scattered light collecting mechanism and a signal analyzing and processing mechanism, wherein the scattered light collecting mechanism comprises a forward scattered light detector and 2-4 90-degree scattered light detectors, and the 90-degree scattered light detectors are arranged on a plane at the laser incidence part vertical to the laser incidence direction, have equal distances from the central line of the sheath flow pipe, and are symmetrical about the central line of the sheath flow pipe. According to the invention, a 90-degree scattered light detecting channel is additionally arranged; the light intensity of an obtained 90-degree scattered light signal is high; the SNR (signal to noise ratio) is improved; the detecting accuracy and the detecting stability are improved; the magnification times and the denoising function of parts of the analyzer can be lowered; the cost of the instrument is lowered; and the reliability is improved.
Description
technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a cell scattered light multi-position detection method and a cell analyzer. Background technique [0002] Flow cytometer (FCM) is a cell analysis instrument based on light scattering theory. When the laser light strikes each cell in a single arrangement flowing through the flow chamber, due to the physical characteristics of the cells, part of the light scatters from the cells at different angles. Among them, the light intensity of forward small-angle scattered light can reflect the cell volume; the light intensity of large-angle scattered light can reflect the complexity of the nucleus and the information of cell particles; and the light intensity of side scattered light can reflect the cell membrane, nuclear membrane, cytoplasm To classify cells and analyze cell DNA and RNA. [0003] The existing flow cytometer uses the sheath flow technology to arran...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
Inventor 唐雪辉
Owner URIT MEDICAL ELECTRONICS CO LTD