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Sebum liposome and preparation method for same

A technology of sebum and plastid, applied in the field of sebum liposome and its preparation

Inactive Publication Date: 2013-09-11
SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the structure of sebum such as ceramide and its superior properties, there will be a lot of room for development in external preparations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Preparation and determination of ceramide liposomes

[0020] HSPC hydrogenated soybean phospholipids, LucasMeyer, Germany; corn ceramide, Shanxi Senfu Biotechnology Co., Ltd.; Sephadex G-50, Amersham Bioxsciences, UK; methanol ethanol, analytical grade; methanol, chromatographically pure.

[0021] Preparation of ceramide liposomes

[0022] Ceramide liposomes were prepared by the reverse-phase evaporation method. In this example, the molar ratio of ceramide: phospholipid was 1:1. Accurately weigh 53.6 mg of corn ceramide and 76.3 mg of HSPC. Dissolve the two in 10ml of ethanol, add a small amount of water, and dissolve in a constant temperature heating magnetic stirrer at 65°C in a water bath. After fully dissolving, add a stirring bar and stir until the solution gradually becomes yellow and viscous. Slowly add 5ml of preheated distilled water with a syringe, cover with plastic wrap, and stir for 20 minutes to obtain milky white ceramide liposo...

Embodiment 2

[0031] The preparation of embodiment 2 ceramide sebum liposomes

[0032] HSPC hydrogenated soybean lecithin, LucasMeyer, Germany; corn ceramide, Shanxi Senfu Biotechnology Co., Ltd.; cholesterol, Nanjing Cenbest Biotechnology Co., Ltd., Sephadex G-50, Amersham Bioxsciences, UK; methanol ethanol, analytically pure; Methanol, chromatographically pure.

[0033] Ceramide liposomes were prepared by the reverse-phase evaporation method. In this example, the molar ratio of ceramide:phospholipid:cholesterol was 10:10:1 for the experiment. Accurately weigh 53.7mg of corn ceramide, 76.3mg of HSPC, and 3.9mg of cholesterol. Melt the three in 10ml of ethanol, add a small amount of water, and dissolve in a constant temperature heating magnetic stirrer at 65°C in a water bath. After fully dissolving, add a stirring bar and stir until the solution gradually becomes yellow and viscous. Slowly add 5ml of preheated distilled water with a syringe, cover with plastic wrap, and stir for ...

Embodiment 3

[0040] The preparation of embodiment 3 ceramide sebum liposomes

[0041] HSPC hydrogenated soybean lecithin, LucasMeyer, Germany; corn ceramide, Shanxi Senfu Biotechnology Co., Ltd.; cholesterol, Nanjing Cenbest Biotechnology Co., Ltd., Sephadex G-50, Amersham Bioxsciences, UK; methanol ethanol, analytically pure; Methanol, chromatographically pure.

[0042] Ceramide liposomes were prepared by the reverse-phase evaporation method. In this example, the molar ratio of ceramide:phospholipid:cholesterol was 5:5:1 for the experiment. Accurately weigh 26.8 mg of corn ceramide, 38.3 mg of HSPC, and 3.8 mg of cholesterol. Melt the three in 10ml of ethanol, add a small amount of water, and dissolve in a constant temperature heating magnetic stirrer at 65°C in a water bath. After fully dissolving, add a stirring bar and stir until the solution gradually becomes yellow and viscous. Slowly add 5ml of preheated distilled water with a syringe, cover with plastic wrap, and stir for 2...

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Abstract

The invention belongs to the technical field of medicines, and relates to a sebum liposome and a preparation method of the same. The sebum liposome comprises ceramide, phospholipid, free fatty acids and cholesterol, wherein the ceramide is a corn extract, namely, corn ceramide, the phospholipids is soya bean lecithin, and the free fatty acids comprise palmitic acid, linoleic acid and oleic acid; the molar ratio of the ceramide to the phospholipid is (1:1)-(9:1), and preferably 2: 1; and the molar ratio of the ceramide or the phospholipid to cholesterol is not greater than 1 / 3, and preferably not greater than 1 / 5. A novel medicine-loading system for the sebum liposome is put forward for the first time; and the system can be used for external preparations, has the advantages of being high in medicine-loading capacity and good in stability, as well as is high in affinity with the skin, and capable of reducing the irritation of the external preparations, enhancing the union of the broken skin, and providing a better application prospect for the external medicine preparations.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to sebum liposomes and a preparation method thereof. Background technique [0002] The main biological barrier that forms the skin is the stratum corneum, and the main lipids in the stratum corneum are composed of complex mixtures such as ceramide, cholesterol and free fatty acids. A large number of studies have shown that ceramides in the skin have the functions of maintaining skin barrier function, moisturizing, repairing, and whitening. At the same time, ceramide can replace phospholipids, and it can be formulated into skin lipids according to the approximate lipid ratio of epidermis stratum corneum, and made into semolipid liposomes. This kind of liposome is more stable than common phospholipid liposomes, and because its lipid composition is similar to that of the stratum corneum of the epidermis, it is easier to penetrate the skin. When the skin lipid li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/28A61P17/00
Inventor 杨跃辉
Owner SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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