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Urease-producing microorganisms and method for solidifying heavy metals in subgrade by using same

A microorganism and urease-producing technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of producing toxic sludge or other wastes, high cost, incomplete removal, etc., and achieve low cost and high effect Good, short-term effect

Active Publication Date: 2013-09-11
TSINGHUA UNIV
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Problems solved by technology

[0003] Traditional heavy metal treatment methods mainly include: chemical precipitation method, ion exchange method, evaporation concentration method, electrolysis method, activated carbon and silica gel adsorption method and membrane separation method, etc., but these methods have incomplete removal, high cost, generation of toxic sludge or Disadvantages such as other waste

Method used

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  • Urease-producing microorganisms and method for solidifying heavy metals in subgrade by using same
  • Urease-producing microorganisms and method for solidifying heavy metals in subgrade by using same
  • Urease-producing microorganisms and method for solidifying heavy metals in subgrade by using same

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Embodiment

[0032] Isolation and Screening of Urease-Producing Bacteria with the Ability to Immobilize Heavy Metals

[0033] 1. Sample collection

[0034] Sporosarcina pasteurii was obtained from the American type culture collection (American type culture collection), numbered ATCC11859, and Terrabacter tumescens was obtained from the General Microbiology Center of China Committee for the Collection of Microorganisms, numbered AS.1.2690.

[0035] 2. Isolation and screening of urease-producing strains

[0036] Urease-producing strains were isolated from soil samples collected from the Tsinghua University flower garden.

[0037] Bacteria have ureolytic enzymes, which can decompose urea to produce a large amount of ammonia, making the medium alkaline and red. This experiment takes advantage of this characteristic. After enriching and culturing the test samples at 37°C and 5M high concentration of urea for 24 hours, kill various microbial vegetative cells that cannot tolerate and utilize hi...

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Abstract

The provided urease-producing microorganisms comprise: Sporosarcina antarctica UR53, Sporosarcina koreensis UR47, Sporosarcina sp. UR31, and Bacillus lentus UR41, wherein all the above microorganisms have been deposited with the China General Microbiological Culture Collection Center (CGMCC) on March 16, 2012, and assigned respectively the accession numbers: CGMCC NO.5916, CGMCC NO.5915, CGMCC NO.5914, and CGMCC NO.5913. The provided method for solidifying heavy metals in subgrade using the urease-producing microorganisms comprises steps of: putting the above four microorganisms, Sporosarcina pasteurii and Terrabacter tumescens in a fermentation medium to obtain a bacterial solution by fermenting; adding a urea reaction solution into the bacterial solution; and adding the solution of the above step into a solution containing heavy metals to form a mixed solution, so that floccules of microorganism-heavy-metal are formed in the mixed solution and water-insoluble heavy-metal-carbonates are formed further. The method for solidifying heavy metals in subgrade using the microorganisms provided by the invention has the advantages of short solidifying time, good effect, low cost, and no secondary pollution to environment.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and solidifying heavy metals thereof, in particular to urease-producing microorganisms and a method for solidifying heavy metals in foundations. Background technique [0002] Heavy metal pollution means that due to human production and activities, the content of heavy metals in the environment is significantly higher than its background value. Due to the long-term retention and non-degradable characteristics of heavy metal ions, it has caused great damage to the ecological environment. With large-scale urban expansion and construction, many buildings are built near abandoned factories and garbage dumps, and the heavy metal pollution in the foundation is serious. Heavy metal elements can finally enter the organism through the food chain, destroy the normal metabolism of the organism, and seriously endanger human health. It has become an environmental problem that cannot be ignored. [0003]...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/07
Inventor 程晓辉李萌郭红仙
Owner TSINGHUA UNIV
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