Method for producing biological de-emulsifying bacteria by using surplus sludge ultrasonic extracting solution
A technology for biological demulsification bacteria and excess sludge, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of restricting the promotion of demulsification bacteria and high production costs, and achieve stable and efficient The effect of high production efficiency and short fermentation time
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] 1. The remaining sludge is taken from the sludge thickening tank of Shanghai Quyang Sewage Treatment Plant. The properties of the sludge are shown in Table 1. The remaining sludge was treated with an ultrasonic cell disruptor. The volume of sludge used for ultrasonication is 0.35L, and the ultrasonic energy is 3540 kJ kg -1 TS, the ultrasonic power and ultrasonic time to achieve ultrasonic energy are 10W-40min, 20W-20min, and 80W-5min, respectively. The ultrasonicated excess sludge was centrifuged in a high-speed centrifuge at a speed of 12000 rpm for 10 minutes, and then filtered through a 0.45 μm microporous membrane to obtain a clarified sludge extract. The concentrations of organic carbon, nitrogen (including ammonia nitrogen and organic nitrogen), phosphorus and various trace elements contained in the sludge extract are shown in Table 2.
[0032] Table 1. Basic information of sludge samples
[0033]
[0034]
[0035] Table 2. Ultrasonic conditions are 3540k...
Embodiment 2
[0047] The difference from Example 1 is that the ultrasonic condition used is 42485kJ·kg -1 The sludge extract of TS was fermented and cultivated for Alcaligenes sp.TR-1, and the ultrasonic power and ultrasonic time to achieve ultrasonic energy were 80W-60min and 100W-48min, respectively. The concentrations of organic carbon, nitrogen (including ammonia nitrogen and organic nitrogen), phosphorus and various trace elements contained in the sludge extract are shown in Table 3.
[0048] Table 3. Ultrasonic conditions are 42485kJ kg -1 TS sludge extract components
[0049]
[0050] The ultrasonic condition for use is 42485kJ·kg -1 The TS sludge extract cultured Alcaligenes sp.TR-1 to obtain a cell yield of 1.93g / L, exceeding 59.5% of the cell cultured on the inorganic salt medium (MMSM). The 24h demulsification efficiency of the bacteria was 84.4%, which was 8.1% different from that of the inorganic salt medium (MMSM). Monitoring of Alcaligenes sp.TR-1 cells under ultrasoni...
Embodiment 3
[0052] The difference from Example 2 is that the volume fraction of paraffin wax added to the sludge extract is 2%. Other operations are the same as in Example 2. The yield of the obtained thallus is 1.66g / L, exceeding 37.5% of the thallus cultured in the inorganic salt medium (MMSM). The 24h demulsification efficiency of the bacteria was 84.0%, which was 8.5% different from that of the inorganic salt medium (MMSM). Other modes of operation are the same as in Embodiment 2.
PUM
| Property | Measurement | Unit |
|---|---|---|
| concentration | aaaaa | aaaaa |
| demulsification efficiency | aaaaa | aaaaa |
| demulsification efficiency | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More