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A secondary salinization soil remediation bacterium and applications thereof

A technology for secondary salinization and soil remediation, which is applied in the field of secondary salinized soil remediation bacteria, can solve the problems of adverse effects on the growth of the next crop, secondary pollution, and high cost, and achieves good application prospects and strong adaptability. , the effect of strong degradation ability

Inactive Publication Date: 2013-09-11
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these measures not only consume a lot of manpower and material resources, but also have adverse effects on the environment.
For example, irrigation and salt washing measures must be based on a high-efficiency drainage system to ensure the free circulation of soil solution, and the loss of pollutants such as nitrogen and phosphorus is more than three times that of open field production, which will cause agricultural non-point source pollution. and other environmental problems; the cost of mulching is high, recycling is difficult, and it may have an adverse effect on the growth of the next crop; precision drip irrigation technology is not only costly but also requires good professional operation techniques, and there are still certain difficulties in large-scale promotion ; The cost of the soil conditioner method is relatively high, and secondary pollution may occur

Method used

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  • A secondary salinization soil remediation bacterium and applications thereof
  • A secondary salinization soil remediation bacterium and applications thereof
  • A secondary salinization soil remediation bacterium and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Isolation and Purification of Klebsiella sp. SNR-1

[0032] The soil samples were taken from the greenhouses in Nanhui Industrial Park, Shanghai, which had been cultivated for 12-15 years, and the samples were thoroughly mixed after sampling. Shake flask enrichment culture was used, and 10 g of soil samples were inoculated into 100 mL enrichment medium, and cultured at 25 °C and 120 rpm. Every 7 days, 10% of the inoculum size was re-inserted into the enriched medium, and so repeated 4 times for acclimatization. The enrichment medium adopts inorganic salt liquid medium, and the composition of inorganic salt liquid medium is glucose 15g, KCl1.0g / L, MgSO 4 ·7H 2 O0.5g / L, CaCl 2 1.0mg / L, FeSO 4 ·7H 2 O0.1g / L, KH 2 PO 4 0.5g / L, pH6.8-7.2, add 15-18g / L agar to the solid medium.

[0033] Take 100 μL of the suspension of domesticated bacteria and spread it on the inorganic salt solid medium, and incubate it upside down in a constant temperature and humidity incubator at...

Embodiment 2

[0036] Identification of Klebsiella sp. SNR-1

[0037] The SNR-1 strain was identified as Klebsiella by comparative analysis of a homologous sequence on 16SrDNA, combined with the strain's utilization of 95 different carbon sources on the Biolog GN2 plate.

[0038] The Klebsiella bacterium was named SNR-1, and it was preserved in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microorganisms on March 22, 2013. Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing , Institute of Microbiology, Chinese Academy of Sciences, the deposit number is CGMCC No.7124.

Embodiment 3

[0040] Detection of Klebsiella sp. SNR-1 Transformation Ability to Nitrate Nitrogen

[0041] 5mL SNR-1 bacteria solution (OD 600=1.1) inoculated in 95mL of 300mg / L inorganic salt medium with nitrate nitrogen concentration. Shake culture at 30°C and 120rpm, and measure the nitrate content and OD of the bacterial solution in the culture medium every 24 hours 600 value.

[0042] the result shows( figure 2 ), with the extension of culture time, the bacterial solution OD 600 The value reached the maximum on the 3rd day; the conversion rate to nitrate nitrogen (300mg / L) reached 92.5% on the 3rd day, and the highest conversion rate to nitrate nitrogen reached 93.5% on the 5th day, and then basically remained stable.

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Abstract

A strain for secondary salinization soil remediation and applications thereof belong to the technical field of secondary salinization soil remediation. The secondary salinization soil remediation strain is Klebsiella sp. (SNR-1), and the accession number thereof is CGMCC No. 7124. The secondary salinization soil remediation strain is used for degrading nitrates. The secondary salinization soil remediation strain of the invention has a good ability of degrading nitrates in soil, and very good adaptability to the environment, thus having good application prospects in salinization soil remediation for protected cultivation.

Description

technical field [0001] The invention relates to a method in the technical field of secondary salinized soil remediation, in particular to a secondary salinized soil remediation bacterium and its application. Background technique [0002] In recent years, facility agriculture has been popularized and applied on a large scale in my country. This intensive use of land has played an important role in the off-season and cross-regional planting of vegetables and other economic crops in our country, and has also contributed to the increase of farmers' income and economic development. made a significant contribution. However, due to the excessive application of chemical fertilizers, unreasonable planting structure, and unreasonable irrigation, the problem of secondary salinization of soil is becoming more and more serious, and has become the main obstacle factor restricting vegetable production. Therefore, how to effectively control and improve soil Salinization pollution has become...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04B09C1/10C12R1/22
Inventor 支月娥周培冯海玮时唯伟张春华李卉
Owner SHANGHAI JIAO TONG UNIV