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Method for improving yield of simultaneous saccharification and fermentation lignocellulose ethanol

A technology of simultaneous saccharification and fermentation, lignocellulose, applied in fermentation, biofuel and other directions, can solve the problems of high water consumption, low ethanol concentration, low efficiency, etc., and achieve the effect of saving water resources, high ethanol concentration, and high solid content

Inactive Publication Date: 2013-09-11
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the existing problems such as large difference in optimum temperature between enzymolysis and fermentation, low efficiency; Method for fermenting lignocellulose ethanol yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for improving the yield of lignocellulose ethanol by synchronous saccharification and fermentation, comprising the steps of:

[0024] Heat corn stalks with steam at 200°C for 10 minutes in a steam explosion reactor, release the pressure instantly to obtain a solid-liquid mixture, separate the solid and liquid, and sterilize the obtained solid at 121°C and 21mim; Mix the sterilized solid, cellulase and sterile water, add yeast powder and peptone, make the concentration of yeast powder after adding 5g / L, make the concentration of peptone after adding 10g / L, adjust pH=5.0, enzyme Decomposition for 36 hours; aseptic operation, inoculate high-temperature-resistant yeast according to the inoculation density of OD4.0, ferment for 168 hours at 42°C and 200 rpm, and separate solid and liquid, and the obtained liquid is a fermentation liquid rich in ethanol. The ethanol concentration is 52g / L, and the ethanol yield is 72% of the theoretical yield.

Embodiment 2

[0026] A method for improving the yield of lignocellulose ethanol by synchronous saccharification and fermentation, comprising the steps of:

[0027] Heat the rice straw with steam at 180°C for 8 minutes in a steam explosion reactor, release the pressure instantly to obtain a solid-liquid mixture, separate the solid and liquid, and sterilize the obtained solid at 121°C and 21mim; Mix the sterilized solid, cellulase and sterile water, adjust the pH=4.0, and enzymatically hydrolyze for 48 hours; aseptically operate, insert high-temperature-resistant yeast according to the inoculation density of OD1.0, and ferment at 39°C and 200rpm 72h, solid-liquid separation, the resulting liquid is a fermented liquid rich in ethanol. The ethanol concentration was 19g / L, and the ethanol yield was 81% of the theoretical yield.

Embodiment 3

[0029] A method for improving the yield of lignocellulose ethanol by synchronous saccharification and fermentation, comprising the steps of:

[0030] Heat the sorghum stalks with steam at 160°C for 6 minutes in a steam explosion reactor, release the pressure instantly to obtain a solid-liquid mixture, separate the solid and liquid, and sterilize the obtained solid at 121°C and 21mim; Mix the sterilized solid, cellulase and sterile water, add yeast powder and peptone, make the concentration after adding yeast powder to 10g / L, make the concentration after adding peptone to be 20g / L, adjust pH=6.0, enzyme Decompose for 24 hours; aseptically operate, inoculate high-temperature-resistant yeast according to the inoculation density of OD8.0, ferment for 96 hours at 36°C and 200 rpm, and separate solid and liquid, and the obtained liquid is a fermentation liquid rich in ethanol. The ethanol concentration is 32g / L, and the ethanol yield is 68% of the theoretical yield.

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Abstract

The invention discloses a method for improving the yield of simultaneous saccharification and fermentation lignocellulose ethanol. The method comprises the following steps of: heating straws in a steam explosion reactor by using steam, releasing the pressure instantaneously so as to obtain a solid-liquid mixture; separating the solid from the liquid; sterilizing the obtained solid by using high-temperature steam; mixing the sterilized solid, cellulase and sterile water, adjusting the pH value and enzymolyzing; introducing high-temperature resisting yeast so as to ferment; separating the solid from the liquid so as to obtain a liquid, namely, a fermented liquid which is rich in ethanol. By utilizing the method, the efficiency of enzymolysis and fermentation is improved; the diastatic fermented solid content is high; the obtained fermented liquid is high in ethanol concentration; the water resource is saved.

Description

technical field [0001] The invention relates to the technical field of biomass new energy, in particular to a method for increasing the yield of synchronous saccharification and fermentation lignocellulose ethanol. Background technique [0002] Alcohol has been produced from grapes, barley and potatoes for thousands of years. In recent years, the production of ethanol from non-starch raw materials, lignocellulose, has become a research hotspot. Bioethanol is a promising fuel that can be produced biologically using a variety of waste materials such as corn stover, rice straw, rice and waste paper. Cellulosic ethanol can be used as a beneficial supplement to fossil energy. China has huge reserves of biomass resources. Every year, agricultural waste straw is about 700 million tons, and other agricultural waste is about 130 million tons; urban solid waste is about 150 million tons every year; and forestry biomass resources can be used for energy purposes every year. About 3 m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10
CPCY02E50/10
Inventor 元英进刘志华李炳志秦磊
Owner TIANJIN UNIV
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