Illegal cooling oil identification method and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
An identification method and a technology for waste oil, which are applied in the field of gene diagnosis, can solve the problems of high detection cost, complicated operation, and low specificity of detection indicators, and achieve the effect of high specificity.
Inactive Publication Date: 2013-09-11
SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
View PDF1 Cites 9 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] The technical problem to be solved by the present invention is to provide a detection index with high specificity, convenient operation, low detection cost and high detection sensitivity in view
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0026] 1.1. Experimental samples
[0027] Oil samples to be inspected: 3 suspected gutter oil samples commissioned by Shenzhen Baoan Public Security Bureau, and the 3 oil samples to be inspected are numbered 1, 2, and 3 respectively. Positive control sample: waste oil sample provided by the National Center for Disease Control and Prevention; negative control sample: salad blend oil purchased from Wal-Mart Supermarket in Shenzhen; blank control sample: deionized water.
[0031] Use nucleic acid lysate to enrich and extract DNA from the oil sample to be tested. The specific operation is as follows: add 5mL of 2% CTAB lysate into a 50mL centrifuge tube, add 3...
Embodiment 2
[0045] 2.1 Oil samples to be inspected: 4 suspected waste oil samples commissioned by Shenzhen Longgang Public Security Bureau, and the 4 oil samples to be inspected are numbered 4, 5, 6 and 7 respectively. Positive control sample: waste oil sample provided by the National Center for Disease Control and Prevention; negative control sample: peanut oil purchased from Shenzhen Wal-Mart Supermarket; blank control sample: deionized water.
[0049] Use the DNeasy Plant Mini Kit kit for nucleic acid enrichment and extraction. The specific operation is as follows: Add 5mL Buffer AP1 into a 50mL centrifuge tube, add 35mL oil sample and mix well, shake at 50°C and 250rpm for 1h, centrifuge at 10000rpm for 5min, r...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to an illegal cooling oil identification method. The method comprises the following steps of: performing deoxyribonucleic acid (DNA) enrichment extraction of an oil sample to be detected; performing enrichment extraction on the obtained DNA by polymerase chain reaction amplification to obtain an amplified DNA segment of the oil sample to be detected; and judging whether the amplified DNA segment of the oil sample to be detected comprises at least one of a plurality of species specificity housekeeping gene segments or not by taking the plurality of species specificity housekeeping gene segments as a detection index, wherein the plurality of species specificity housekeeping gene segments are species specificity housekeeping gene segments of non-oil plants or species specificity housekeeping gene segments of animals. According to the method provided by the invention, the species specificity housekeeping gene segments of the non-oil plants serve as the detection index, so high specificity of the detection index is guaranteed; the detection sensitivity of the method provided by the invention is improved by the polymerase chain reaction; the DNA extraction efficiency of the oil sample to be detected is obviously improved after DNA enrichment treatment; and compared with the prior art, the illegal cooling oil identification method has the advantages of low equipment cost and simple and convenient detection method.
Description
technical field [0001] The present invention relates to gene diagnosis technology, more specifically, relates to a waste oil identification method and application thereof. Background technique [0002] Waste oil, also known as "kitchen waste oil", "swill oil" or "swill oil", refers to non-edible oil produced by refining kitchen waste. The raw materials for extracting waste oil mainly come from leftovers from hotels and restaurants, leftovers (commonly known as swill) or greasy floating objects in sewers; inferior pigs, cattle, mutton, skin and viscera, and old products after repeated use of high-temperature fried foods. kerosene. Waste oil contains carcinogenic substances such as aflatoxin and high cholesterol content, which may cause abdominal pain, digestive system cancer and other diseases after consumption. my country has explicitly prohibited waste oil from returning to the catering industry. However, the illegal sale or use of waste oil as or mixed with normal edible...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
IPC IPC(8): C12Q1/68
Inventor 邓平建杨冬燕杨永存李浩
Owner SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
Login to view more 
Login to view more