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Illegal cooling oil identification method and application thereof

An identification method and a technology for waste oil, which are applied in the field of gene diagnosis, can solve the problems of high detection cost, complicated operation, and low specificity of detection indicators, and achieve the effect of high specificity.

Inactive Publication Date: 2013-09-11
SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a detection index with high specificity, convenient operation, low detection cost and high detection sensitivity in view of the problems of low detection index specificity, complicated operation and high detection cost in the identification method in the prior art. High waste oil identification method and its application

Method used

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  • Illegal cooling oil identification method and application thereof
  • Illegal cooling oil identification method and application thereof
  • Illegal cooling oil identification method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1.1. Experimental samples

[0027] Oil samples to be inspected: 3 suspected gutter oil samples commissioned by Shenzhen Baoan Public Security Bureau, and the 3 oil samples to be inspected are numbered 1, 2, and 3 respectively. Positive control sample: waste oil sample provided by the National Center for Disease Control and Prevention; negative control sample: salad blend oil purchased from Wal-Mart Supermarket in Shenzhen; blank control sample: deionized water.

[0028] 1.2 Main reagents and equipment

[0029] CTAB lysate, chloroform, isoamyl alcohol, absolute ethanol, Tris-EDTA solution, fluorescent PCR kit (Dalian Bao Biological Company), centrifuge (Eppendorf, Germany), fluorescent PCR amplification instrument (ABI7500, USA).

[0030] 1.3 Nucleic acid enrichment and extraction

[0031] Use nucleic acid lysate to enrich and extract DNA from the oil sample to be tested. The specific operation is as follows: add 5mL of 2% CTAB lysate into a 50mL centrifuge tube, add 3...

Embodiment 2

[0045] 2.1 Oil samples to be inspected: 4 suspected waste oil samples commissioned by Shenzhen Longgang Public Security Bureau, and the 4 oil samples to be inspected are numbered 4, 5, 6 and 7 respectively. Positive control sample: waste oil sample provided by the National Center for Disease Control and Prevention; negative control sample: peanut oil purchased from Shenzhen Wal-Mart Supermarket; blank control sample: deionized water.

[0046] 2.2 Main reagents and equipment

[0047] DNeasy Plant Mini Kit Nucleic Acid Extraction Kit (QIAGEN, Germany), PCR Kit (Dalian Bao Biological Company), Centrifuge (Eppendorf, Germany), PCR Amplifier (MJRPTC-220, USA).

[0048] 2.3 Nucleic acid enrichment and extraction

[0049] Use the DNeasy Plant Mini Kit kit for nucleic acid enrichment and extraction. The specific operation is as follows: Add 5mL Buffer AP1 into a 50mL centrifuge tube, add 35mL oil sample and mix well, shake at 50°C and 250rpm for 1h, centrifuge at 10000rpm for 5min, r...

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Abstract

The invention relates to an illegal cooling oil identification method. The method comprises the following steps of: performing deoxyribonucleic acid (DNA) enrichment extraction of an oil sample to be detected; performing enrichment extraction on the obtained DNA by polymerase chain reaction amplification to obtain an amplified DNA segment of the oil sample to be detected; and judging whether the amplified DNA segment of the oil sample to be detected comprises at least one of a plurality of species specificity housekeeping gene segments or not by taking the plurality of species specificity housekeeping gene segments as a detection index, wherein the plurality of species specificity housekeeping gene segments are species specificity housekeeping gene segments of non-oil plants or species specificity housekeeping gene segments of animals. According to the method provided by the invention, the species specificity housekeeping gene segments of the non-oil plants serve as the detection index, so high specificity of the detection index is guaranteed; the detection sensitivity of the method provided by the invention is improved by the polymerase chain reaction; the DNA extraction efficiency of the oil sample to be detected is obviously improved after DNA enrichment treatment; and compared with the prior art, the illegal cooling oil identification method has the advantages of low equipment cost and simple and convenient detection method.

Description

technical field [0001] The present invention relates to gene diagnosis technology, more specifically, relates to a waste oil identification method and application thereof. Background technique [0002] Waste oil, also known as "kitchen waste oil", "swill oil" or "swill oil", refers to non-edible oil produced by refining kitchen waste. The raw materials for extracting waste oil mainly come from leftovers from hotels and restaurants, leftovers (commonly known as swill) or greasy floating objects in sewers; inferior pigs, cattle, mutton, skin and viscera, and old products after repeated use of high-temperature fried foods. kerosene. Waste oil contains carcinogenic substances such as aflatoxin and high cholesterol content, which may cause abdominal pain, digestive system cancer and other diseases after consumption. my country has explicitly prohibited waste oil from returning to the catering industry. However, the illegal sale or use of waste oil as or mixed with normal edible...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 邓平建杨冬燕杨永存李浩
Owner SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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