Illegal cooling oil identification method and application thereof

An identification method and a technology for waste oil, which are applied in the field of gene diagnosis, can solve the problems of high detection cost, complicated operation, and low specificity of detection indicators, and achieve the effect of high specificity.
CN103290103AInactive Publication Date: 2013-09-11SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
1 Cites 9 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
Publication Date
2013-09-11
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to an illegal cooling oil identification method. The method comprises the following steps of: performing deoxyribonucleic acid (DNA) enrichment extraction of an oil sample to be detected; performing enrichment extraction on the obtained DNA by polymerase chain reaction amplification to obtain an amplified DNA segment of the oil sample to be detected; and judging whether the amplified DNA segment of the oil sample to be detected comprises at least one of a plurality of species specificity housekeeping gene segments or not by taking the plurality of species specificity housekeeping gene segments as a detection index, wherein the plurality of species specificity housekeeping gene segments are species specificity housekeeping gene segments of non-oil plants or species specificity housekeeping gene segments of animals. According to the method provided by the invention, the species specificity housekeeping gene segments of the non-oil plants serve as the detection index, so high specificity of the detection index is guaranteed; the detection sensitivity of the method provided by the invention is improved by the polymerase chain reaction; the DNA extraction efficiency of the oil sample to be detected is obviously improved after DNA enrichment treatment; and compared with the prior art, the illegal cooling oil identification method has the advantages of low equipment cost and simple and convenient detection method.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to gene diagnosis technology, more specifically, relates to a waste oil identification method and application thereof. Background technique

[0002] Waste oil, also known as "kitchen waste oil", "swill oil" or "swill oil", refers to non-edible oil produced by refining kitchen waste. The raw materials for extracting waste oil mainly come from leftovers from hotels and restaurants, leftovers (commonly known as swill) or greasy floating objects in sewers; inferior pigs, cattle, mutton, skin and viscera, and old products after repeated use of high-temperature fried foods. kerosene. Waste oil contains carcinogenic substances such as aflatoxin and high cholesterol content, which may cause abdominal pain, digestive system cancer and other diseases after consumption. my country has explicitly prohibited waste oil from returning to the catering industry. However, the illegal sale or use of waste oil as or mixed with normal edible...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More