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Detection method of human platelet allogeneic antigen gene

A detection method and alloantigen technology, applied in the field of platelet detection, can solve the problems of operator health threat, time-consuming and labor-intensive, human injury, etc., and achieve the effect of shortening detection time and improving sensitivity

Inactive Publication Date: 2013-09-11
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

The technology itself is time-consuming and labor-intensive, and it needs to use toxic dyes such as EB to complete the imaging process.
It can cause great harm to the human body and pose a huge threat to the health of the operator

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  • Detection method of human platelet allogeneic antigen gene

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Embodiment Construction

[0024] The preferred embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0025] The invention provides a detection method for human platelet alloantigen gene, comprising the steps of:

[0026] (1) The primers are labeled with biotin, and the primers are designed for specific amplification sequences such as the HPA-1 gene sequence. The specific primers can be one of the primers, or two of them can be labeled at the same time;

[0027] (2) Put the biotin-labeled primer into the polymerase chain reaction instrument, and carry out gene amplification through the ordinary polymerase chain reaction system. If the target gene is contained, the amplified product will contain a large amount of biotin. Primed amplified sequence 2;

[0028] (3) Take out part of the amplified pr...

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Abstract

The invention discloses a detection method of a human platelet allogeneic antigen gene. The detection method comprises the following steps of: carrying out polymerase chain reaction on a primer marked by a first marker to obtain an amplification product; taking partial amplification product out and adding the partial amplification product into a buffer solution to obtain a mixture, wherein the buffer solution comprises a carrier marked by a second marker and a probe marked by a third marker; dropping the mixture to a reagent strip to obtain a detection result, wherein the reagent strip comprises a biological substance which can be specifically bound with the third marker. The detection method of the human platelet allogeneic antigen gene provided by the invention carries out HPA (Human Platelet Antigen) gene amplification by means of PCR (Polymerase Chain Reaction) method, achieves the fast typing of the HPA gene by using a lateral flow immune chromatography technology in combination, shortens detection time, improves detection sensitivity and is of great clinical significance and value in the fast typing of the HPA gene.

Description

technical field [0001] The invention relates to the field of platelet detection, in particular to a detection method for human platelet alloantigen gene. Background technique [0002] Platelets were discovered and named for the first time by the Italian physician Bizzozero in 1882, and they play an important role in the mechanism of blood hemostasis. In recent years, with the promotion and application of component blood transfusion in clinical practice, the proportion of platelet dosage in blood transfusion therapy has been increasing year by year, and it has been widely used in bleeding diseases caused by primary and secondary thrombocytopenia or platelet function defect. Maintain the normal hemostasis and coagulation function of the human body, and become one of the indispensable treatments for various blood diseases, tumor patients after high-dose chemotherapy or radiotherapy, and bone marrow transplantation, which can reduce or reduce the serious complications and mortal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 尹焕才
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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