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Visual membrane receptor chip, preparation method and applications thereof

A sensor and chip technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as expensive instruments and equipment, and achieve the effects of low price, strong contrast between signal and background, and strong specificity

Inactive Publication Date: 2011-07-20
PEKING UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

This type of method is used in many research fields, but it requires expensive instruments and equipment, and requires specially trained researchers to operate, thus limiting its application in a wide range of routine inspections and ordinary experimental research

Method used

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  • Visual membrane receptor chip, preparation method and applications thereof
  • Visual membrane receptor chip, preparation method and applications thereof
  • Visual membrane receptor chip, preparation method and applications thereof

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Embodiment Construction

[0058] The present invention will be further described below through specific experiments and results thereof, but the scope of the present invention will not be limited in any way.

[0059] Materials and Methods

[0060] 1. Design and fabrication of thin-film sensor chip with silver background visible

[0061] Visible thin film susceptor chip with a diameter of 10 cm, the surface is covered with a specific thickness of Si 3 N 4 The film layer is specifically divided into four intervals of 50-70nm, 110-140nm, 250-270nm or 320-340nm. Coat 10-15nm T-type polyamino basic polydimethylsiloxane on the surface of the wafer by spin coating, and then soak the wafer in 50 μg / ml polyphenylalanine-lysine solution for 1- For 24 hours, the wafer was allowed to absorb polyphenylalanine-lysine, and then the wafer was rinsed and dried. The wafer containing polyphenylalanine-lysine was treated (i.e. soaked) in 25 mL of 0.1 M sodium borate (pH 8.2) containing 1-10 μM succinimidyl nicotinic a...

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Abstract

The invention provides a visual membrane receptor chip, a preparation method and the applications thereof. The preparation method of the chip comprises the following steps of: making a membrane Si3N4 with a specific thickness on the surface of a silicon substrate so that a silver background presents on the surface of the silicon substrate; further coating a T-shaped polyamino alkaline polydimethylsiloxane membrane in a spinning way; and activating. A specific nucleotide sequence and SNP (Single Nucleotide Polymorphism) points (or point mutation) are detected by utilizing the visual membrane receptor chip with a silver background. When a specific target sequence is detected, the chip surface has visually recognizable color change from silver to blue or red. The detection technology is rapid, accurate and extremely economical and has low cost, high sensitivity and strong specificity and free selection between low flux and high flux. Compared with the visual membrane receptor chip with a gold background or the like, the visual membrane receptor chip provided by the invention ensures that the silver background provides stronger color comparison and has a clearer result in case of a blue signal, and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the preparation of a visible film sensor chip and its application in rapid detection and identification of specific nucleotide sequences and single nucleotide polymorphisms in organisms. Background technique [0002] In the field of life science research, it is necessary to be able to quickly and accurately identify specific nucleotides. At present, the identification of nucleotides to distinguish different species and even different individuals has been widely used. Customs and the government often need to test whether the genetic components in organisms or biological products meet the corresponding regulatory requirements. With the increasing research and marketization of transgenic breeding, the requirements for the detection of exogenous gene sequences in organisms or products are increasing. In order to meet the above various needs, it is necessary to develop assay methods wit...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 邓兴旺陈浩东杨洋唐达芳
Owner PEKING UNIV
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