Nucleic acid nano-gold biosensor for detecting mercury ions and kit

A biosensor and nucleic acid nanotechnology, applied in the field of nucleic acid nanogold biosensors, can solve the problems of expensive instruments and complicated operations, and achieve the effects of rapid detection, simple preparation, and high sensitivity
CN103290132AActive Publication Date: 2013-09-11GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
Publication Date
2013-09-11

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Abstract

The invention relates to a nucleic acid nano-gold biosensor for detecting mercury ions and a corresponding kit. The nucleic acid nano-gold biosensor comprises a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water-absorbing pad, wherein the nitrocellulose membrane is provided with a detection line and a quality control line; colloidal gold is sprayed on the gold-labeled pad; the detection line is marked on the nitrocellulose membrane after streptavidin and a biotin-labeled SEQ ID NO:3 sequence react; the quality control line is marked on the nitrocellulose membrane after streptavidin and a biotin-labeled SEQ ID NO:4 sequence react; colloidal gold is bonded to an SEQ ID NO:2 sequence, and 5'-terminal of the sequence is modified by sulfydryl. According to the nucleic acid nano-gold biosensor and the corresponding kit, the mercury ions are detected rapidly, simply, conveniently and directly, the defect that a large instrument is required in the conventional detection method is overcome, and the detection sensitivity can be guaranteed.
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Description

technical field

[0001] The invention relates to the field of metal ion detection, in particular to a nucleic acid nano-gold biosensor for detecting mercury ions and a corresponding kit. Background technique

[0002] At present, the traditional methods for detecting mercury ions at home and abroad mainly include spectroscopic analysis, mass spectrometry and chromatography. Spectroscopic analysis refers to the analytical methods that use the principles and experimental methods of spectroscopy to determine the structure and chemical composition of substances, including visible spectrophotometry, atomic absorption spectroscopy, atomic fluorescence spectroscopy, atomic emission spectroscopy, etc. Visible spectrophotometry analysis of mercury ions has low sensitivity and low selectivity, and requires the use of highly toxic substances and volatile organic solvents, which are potentially harmful to experimenters. The experimental conditions of flame atomic absorption spectrometry ...

Claims

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