Method for obtaining virus-free seedlings of pure cymbidium efficiently

A seedling and high-efficiency technology, which is applied in the field of efficiently obtaining virus-free seedlings of Suxinjianlan, can solve the problems of long cycle, low reproduction coefficient, easy injury and mother plant, etc., and achieve the goal of improving success rate, reproduction coefficient and germination rate Effect

Inactive Publication Date: 2013-09-18
葛建
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Problems solved by technology

[0003] There are three main propagation techniques of Suxinjianlan: ramet propagation, seed propagation and tissue culture propagation. The ramet propagation speed is very slow, and the reproduction coefficient is low, the cycle is long, and it

Method used

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Embodiment 1

[0038] A method for efficiently obtaining Suxinjianlan detoxification seedlings, the specific steps are as follows:

[0039] a. Seed germination induction of Suxinjianlan

[0040] Choose Suxinjianlan capsules with better living conditions, remove them, and place them in a 3-5°C refrigerator for 1 week to make the seeds mature; cut the capsules with scissors to take out the seeds, and place the seeds in a very fine hole. In the polypropylene sieve, disinfected with 10% hydrogen peroxide for 10 minutes, rinsed repeatedly with sterile water for 3 times after taking out, and drained the water; then treated with 0.1% mercuric solution for 2 minutes, and rinsed repeatedly with sterile water after taking out 3 times, drain the water; finally, the seeds were sterilized in 75% alcohol for 30s, rinsed with sterile water for 3 times after treatment, and drained; the treated seeds were sown in the modified MS solid medium, and then The medium sown with the seeds of Suxinjianlan was cultu...

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Abstract

The invention discloses a method for obtaining virus-free seedlings of pure cymbidium efficiently. The method includes: selecting pure cymbidium capsules with good living conditions, implanting the capsules in an MF solid culture medium to sprout after disinfection treatment of 15% hydrogen peroxide, 0.1% mercury bichloride and 75% ethyl alcohol, scissoring bud tips, stem tips or root tips of pure cymbidium seedlings after seeds sprout and grow to form 2 tender leaves, putting the bud tips, the stem tips or the root tips of the pure cymbidium seedlings in a induction protocorm culture medium MY after disinfection treatment to perform dedifferentiation culture to form protocorms, taking out the protocorms, cutting the protocorms into 1mm <2> blocks, implanting the blocks in a protocorm redifferentiation induction medium MZ to form buds and novel roots through induction, opening aseptic bottle caps of plant tissue culture bottles when plants of the seedlings grow up to 4cm-6cm and each seedling has 5-6 roots, taking the bottles to an illumination culture frame to be cultured, and performing habituated culture gradually. According to the method, success rate of pure cymbidium seedling culture can be improved, breeding period can be shortened greatly, and virus-free pure cymbidium seedlings can be obtained.

Description

technical field [0001] The invention relates to a method for efficiently obtaining the detoxification seedlings of Orchid similis, in particular to a method for efficiently obtaining the detoxification seedlings of Orchid spp. Background technique [0002] Suxin Jianlan is an important variety of the national treasure Cymbidium. Suxin Jianlan is a perennial herb, which is a hybrid of some epiphytic species and a small part of terrestrial orchid species in the genus Orchid. , The flowers are often light yellow-green, with deep purple-red veins and spots. The flowers are usually rich in aroma and generally live in temperate and subtropical zones. Because of their beautiful shape, they are often used as potted plants and are a good product for indoor decoration. [0003] The propagation techniques of Suxinjianlan mainly consist of three types: ramet propagation, seed propagation and tissue culture propagation. The ramet propagation speed is very slow, the reproduction coefficie...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 葛建
Owner 葛建
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