Method for obtaining virus-free seedlings of pure cymbidium efficiently

Abstract

The invention discloses a method for obtaining virus-free seedlings of pure cymbidium efficiently. The method includes: selecting pure cymbidium capsules with good living conditions, implanting the capsules in an MF solid culture medium to sprout after disinfection treatment of 15% hydrogen peroxide, 0.1% mercury bichloride and 75% ethyl alcohol, scissoring bud tips, stem tips or root tips of pure cymbidium seedlings after seeds sprout and grow to form 2 tender leaves, putting the bud tips, the stem tips or the root tips of the pure cymbidium seedlings in a induction protocorm culture medium MY after disinfection treatment to perform dedifferentiation culture to form protocorms, taking out the protocorms, cutting the protocorms into 1mm <2> blocks, implanting the blocks in a protocorm redifferentiation induction medium MZ to form buds and novel roots through induction, opening aseptic bottle caps of plant tissue culture bottles when plants of the seedlings grow up to 4cm-6cm and each seedling has 5-6 roots, taking the bottles to an illumination culture frame to be cultured, and performing habituated culture gradually. According to the method, success rate of pure cymbidium seedling culture can be improved, breeding period can be shortened greatly, and virus-free pure cymbidium seedlings can be obtained.

Description

technical field [0001] The invention relates to a method for efficiently obtaining the detoxification seedlings of Orchid similis, in particular to a method for efficiently obtaining the detoxification seedlings of Orchid spp. Background technique [0002] Suxin Jianlan is an important variety of the national treasure Cymbidium. Suxin Jianlan is a perennial herb, which is a hybrid of some epiphytic species and a small part of terrestrial orchid species in the genus Orchid. , The flowers are often light yellow-green, with deep purple-red veins and spots. The flowers are usually rich in aroma and generally live in temperate and subtropical zones. Because of their beautiful shape, they are often used as potted plants and are a good product for indoor decoration. [0003] The propagation techniques of Suxinjianlan mainly consist of three types: ramet propagation, seed propagation and tissue culture propagation. The ramet propagation speed is very slow, the reproduction coefficie...

AI Technical Summary

Problems solved by technology

[0003] There are three main propagation techniques of Suxinjianlan: ramet propagation, seed propagation and tissue culture propagation. The ramet propagation speed is very slow, and the reproduction coefficient is low, the cycle is long, and it is easy to damage the mother plant; the seeds of Suxinjianlan are extremely small, The seeds contain endosperm, and the germination rate is extremely low under natural conditions, which is difficult to meet the needs of large-scale production