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Stellera chamaejasmine compounded pesticide as well as preparation method and applications thereof

A technology of Daphne chamaejasmin and insecticide, which is applied in the field of Daphne chamaejasmin compound insecticide and its preparation method and application, can solve the problem of high cost, achieve the effects of reducing consumption, reducing pollution, and saving costs

Active Publication Date: 2015-07-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chamaejasma extract in its Daphne chamaejasma microemulsion accounts for 10-20wt% of the raw material, and the cost is relatively high

Method used

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  • Stellera chamaejasmine compounded pesticide as well as preparation method and applications thereof
  • Stellera chamaejasmine compounded pesticide as well as preparation method and applications thereof
  • Stellera chamaejasmine compounded pesticide as well as preparation method and applications thereof

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preparation example Construction

[0031] Furthermore, the present invention also discloses a preparation method of Daphne chamaejasmine compound insecticide suspension, including the following steps:

[0032] a. Weigh Daphne chamaejasmin and auxiliary agents according to the proportion, mix and grind to obtain mixed raw materials;

[0033] b. emulsifying the mixed raw materials obtained in step a to obtain a Bt-free suspending agent;

[0034] c. Add Bt and water, stir, and obtain.

[0035] Further, the present invention also discloses a preparation method of Daphne chamaejasmin compound insecticide microemulsion, comprising the following steps:

[0036] a. Add water, daphne chamaejasmin and stabilizer according to the ratio, and stir; wherein, the reaction temperature is 40°C;

[0037] b, add emulsifier and cosolvent, stir, obtain the microemulsion that does not contain Bt;

[0038] c. Add Bt and stir to obtain.

[0039] Wherein, the Daphne chamaejasmin described in the present invention can be extracted by ...

Embodiment 1

[0042] Embodiment 1 adopts Daphne chamaejasmin compound insecticide of the present invention to carry out indoor bioassay test

[0043] The contact activity adopts the dipping method, put the 3rd instar locusts into the filter screen, immerse them in the liquid medicine of different concentrations, take them out quickly after 1 second, put them back into the breeding cages for feeding, and use water as a control, insert 40 locusts in each cage, and repeat 3 times. Group, observed 72h death. The data were statistically processed with SPSS software. Calculation of mortality, corrected mortality and lethal concentration (LC 50 ), virulence regression equation, and the co-toxicity coefficient (CTC) was calculated by Sun Yunpei's method.

[0044]

[0045]

[0046] (Using Daphne chamaejasmin as the standard drug, its toxicity index is 100)

[0047] Toxicity index (TI) of B = LC of A 50 / B LC 50 ×100

[0048] Actual Toxicity Index (ATI) of M = LC of A 50 / M LC 50 ×100 ...

Embodiment 2

[0058] Embodiment 2 adopts compound agent of the present invention to carry out grassland test

[0059] Through the indoor biotesting test, the proportion six with the largest CTC was selected to carry out the field test for controlling locusts, so as to determine the control effect of the combination of Daphne chamaejasmine and Bt on locusts; Compare the effect of the control to verify the synergistic effect after compounding. In this embodiment, 1.2% Daphne chamaejamajasin·Bt suspension concentrate is selected, wherein the weight ratio of Daphne chamaejasmine:Bt is 1.2:4.

[0060] 1. Experimental location:

[0061] Songshutang Grassland Locust Infestation Area under the Experimental Station of Songshutang Grassland Locust Monitoring and Control in Hami City, Xinjiang.

[0062] 2. Test method:

[0063] First select a plot with relatively strong representativeness and relatively uniform locust density as the test area, and then randomly sample and investigate the locust den...

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Abstract

The invention relates to a stellera chamaejasmine compounded pesticide as well as a preparation method and an applications thereof, belonging to the field of biopesticides. The invention aims at solving the technical problem of providing the stellera chamaejasmine compounded pesticide as well as the preparation method and the applications thereof. The stellera chamaejasmine compounded pesticide contains effective components of stellera chamaejasmine and Bt, wherein Bt refers to bacillus thuringiensis; the concentration unit of Bt is 5000IU / mg; the weight ratio of bacillus thuringiensis to Bt is (1-8):(1-64), preferably 1:(1-8) and more preferably 1:(2-4). The invention also discloses the preparation method of the stellera chamaejasmine compounded pesticide as well as the applications of the stellera chamaejasmine compounded pesticide in killing locusts. The pesticide compounded by stellera chamaejasmine and Bt is high in effect and low in toxicity and has an excellent insecticidal activity to pests (especially locusts); meanwhile, the usage amounts of the effectively components are greatly reduced, the cost is saved, the generation of pesticide resistance is slowed and the pollution to environment is alleviated; the pesticide is safe and harmless to plants.

Description

technical field [0001] The invention relates to Daphne chamaejasmin compound insecticide and its preparation method and application, belonging to the field of biological pesticides. Background technique [0002] Daphne chamaejasmin (bayertoxin, daphnetin) is a plant pesticide mother liquor obtained by extracting and separating Daphne chamaejasme. Daphne chamaejasma contains a variety of insecticidal ingredients, which have good antifeedant activity, contact killing activity and growth inhibition activity on pests. It can effectively control pests of Homoptera, Lepidoptera, and Acarina such as aphids, borers, cabbage caterpillars, and mites. Daphne chamaejasmine is a natural extract, which is safe and non-toxic to plants. It is a biopesticide with high safety, high efficiency and low residue, and is suitable for use in vegetables, fruits, and gardening. [0003] Bacillus thuringiensis (Bt for short, 5000IU / mg) is a gram-positive Agrobacterium belonging to the genus Bacillus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/08A01P7/04
Inventor 侯太平陶科高嫚潞吴朗
Owner SICHUAN UNIV