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Method for preparing compound enzyme and probiotic preparation for feed by biotransformation of reed and peanut cake

A technology of probiotics preparation and peanut cake, which is applied in the direction of animal feed, animal feed, application, etc., can solve the problem of difficult digestion of monogastric animals, and achieve low-cost and high-efficiency biotransformation, high enzyme activity, and strong enzyme-producing ability. Effect

Inactive Publication Date: 2015-01-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu’er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of reeds, 5kg of peanut cakes, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, and cultivate them at 35°C After 48 hours, a leavening agent is formed;

[0028] 2) Fully mix 20kg of starter in step 1) with 600kg of reeds, 300kg of peanut cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C for 4 Day, get the compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 48.58IU / g; total cellulase activity reaches 4.87IU / g; beta-glucosidase activity reaches 4.87IU / g; reached 12.12IU / g; endoglucanase activity reached 39.85IU / g; xylanase activity reached 41.51IU / g; beta-xyl...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of reeds, 5kg of peanut cakes, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and cultivate them at 35°C After 48 hours, a leavening agent is formed;

[0032] 2) Fully mix 20kg of starter in step 1) with 600kg of reeds, 300kg of peanut cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C for 4 Day, get the compound enzyme and probiotic preparation for feed.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 48.19IU / g; total cellulase activity reaches 4.96IU / g; beta-glucosidase activity reaches 4.96IU / g; reached 12.08IU / g; endoglucanase activity reached 39.64IU / g; xylanase activity reached 41.48IU / g; beta-xy...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu-erh cooked tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of reeds, 5kg of peanut cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and cultivate them at 40°C After 36 hours, a leavening agent is formed;

[0036] 2) Mix 10kg of starter in step 1) with 600kg of reeds, 300kg of peanut cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and ferment at 35°C for 5 days , to obtain the compound enzyme and probiotic preparation for feed.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 47.70IU / g; total cellulase activity reaches 5.22IU / g; beta-glucosidase activity reaches 5.22IU / g; reached 11.84IU / g; endoglucanase activity reached 41.12IU / g; xylanase activity reache...

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PUM

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Abstract

The invention discloses a method for preparing a compound enzyme and probiotic preparation for feed by biotransformation of reed and peanut cake. The method comprises the following steps of: fully mixing Pu'er ripe tea, shiitake mushroom log, agrocybe aegerita mushroom log, first reed, first peanut cake, first wheat bran, first apple pomace and first water which are fermented by piling, and performing acclimation culture so as to form a fermentation agent; and fully mixing the fermentation agent, second reed, second peanut cake, second wheat bran, second apple pomace and second water, and fermenting so as to obtain the compound enzyme and probiotic preparation for feed. In the method disclosed by the invention, a food-grade safe bacterial group with stable microecology is utilized for fermenting the reed and the peanut cake so as to prepare the compound enzyme and probiotic preparation for feed, which has various better performance indexes, and the low-cost and high-efficient biotransformation of the reed and the peanut cake can be realized. Simultaneously, the method disclosed by the invention has the advantages of easiness in growth of microbes, high enzyme activity, extensive fermentation process and easiness in industrial large-scale production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for preparing compound enzyme and probiotic preparation for feed by adopting microecologically stable food-grade safe flora to ferment reed and peanut cake. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellulose, lign...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖徐娟李青青于骅陈功诸文颖杨璐杜姗姗吴渊纪晓燚
Owner ZHEJIANG UNIV
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